Western Blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used in molecular biology, immunogenetics and other molecular biology disciplines to detect specific proteins in a sample of tissue homogenate or extract. In brief, the sample undergoes protein denaturation, followed by gel electrophoresis. Synthetic or animal-derived antibodies are created that recognise and bind to a specific target protein, known as the primary antibody. The electrophoresis membrane is washed in a solution containing the primary antibody, before excess antibody is washed off. A secondary antibody is added which recognises and binds to the primary antibody. The secondary antibody is visualised through various methods such as staining, immunofluorescence, and radioactivity, allowing indirect detection of the specific target protein.
What's Popular in Western Blot
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JAN 27, 2019Neuroscience
JAN 27, 2019A study out of John's Hopkins University published by Dr. Faith Dickerson, MD, and colleagues late last year have found a possible biomarker. Biom...WRITTEN BY: Amy LoriauxOCT 29, 2018Neuroscience
OCT 29, 2018Undersatnding the role of gut microbiome in disease pathologies and targetting them for potential treatment strategies....WRITTEN BY: Aswini Kanneganti
JAN 27, 2019
Neuroscience
JAN 27, 2019
A study out of John's Hopkins University published by Dr. Faith Dickerson, MD, and colleagues late last year have found a possible biomarker. Biom...
WRITTEN BY:
Amy Loriaux
OCT 29, 2018
Neuroscience
OCT 29, 2018
Undersatnding the role of gut microbiome in disease pathologies and targetting them for potential treatment strategies....
WRITTEN BY:
Aswini Kanneganti
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NOV 05, 2019 07:00 AM PSTAdd to Calendar Select one of the following:
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NOV 05, 2019 07:00 AM PSTAdd to Calendar Select one of the following:
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DATE: November 5, 2019 TIME: 7:00am PDT, 9:00am CSTDetection of low-abundance proteins and/or detection of proteins from precious samples can be a major challenge when performing wes...Speaker: Emily HalbraderSponsored By: Thermo Fisher ScientificNOV 18, 2019 07:00 AM PSTAdd to Calendar Select one of the following:
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NOV 18, 2019 07:00 AM PSTAdd to Calendar Select one of the following:
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DATE: November 18, 2019TIME: 7:00am PST, 11:00am EST, 4:00pm CEWT How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop th...Speaker: Nadine Mellies, PhD , Katja Baesler, PhDSponsored By: EppendorfDEC 17, 2019 09:00 AM PSTAdd to Calendar Select one of the following:
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DEC 17, 2019 09:00 AM PSTAdd to Calendar Select one of the following:
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DATE: December 17, 2019TIME: 9:00am PST, 12:00pm EST Purpose: Liver x receptors (LXRs) are hypothesized to serve as a link between lipid metabolism and inflammation by prom...Speaker: Sandra HammerSponsored By: Thermo Fisher Scientific/GibcoSEP 24, 2019 09:00 AM PDTC.E. CREDITS
SEP 24, 2019 09:00 AM PDTDATE: September 24, 2019TIME: 9:00am PDTThis webinar will cover the basics of the Milo Single-Cell Western blot (SCW) and RNAScope single-molecule fluorescence RNA In situ hybridizati...Speaker: Brian P. Hermann, PhDSponsored By: Advanced Cell DiagnosticsSEP 05, 2019 04:00 PM CESTC.E. CREDITS
SEP 05, 2019 04:00 PM CESTDATE: September 5, 2019TIME: 7:00am PT, 10:00am ET, 4:00pm CEST PCR (Polymerase Chain Reaction) has gone through a massive evolution since its development in 1983. Besides it...Speaker: Tim Schommartz, PhDSponsored By: Eppendorf
NOV 05, 2019
07:00 AM PST
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NOV 05, 2019
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DATE: November 5, 2019 TIME: 7:00am PDT, 9:00am CSTDetection of low-abundance proteins and/or detection of proteins from precious samples can be a major challenge when performing wes...
Speaker:
Emily Halbrader
Sponsored By: Thermo Fisher Scientific
NOV 18, 2019
07:00 AM PST
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NOV 18, 2019
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DATE: November 18, 2019TIME: 7:00am PST, 11:00am EST, 4:00pm CEWT How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop th...
Speaker:
Nadine Mellies, PhD
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Katja Baesler, PhD
Sponsored By: Eppendorf
DEC 17, 2019
09:00 AM PST
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DEC 17, 2019
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DATE: December 17, 2019TIME: 9:00am PST, 12:00pm EST Purpose: Liver x receptors (LXRs) are hypothesized to serve as a link between lipid metabolism and inflammation by prom...
Speaker:
Sandra Hammer
Sponsored By: Thermo Fisher Scientific/Gibco
SEP 24, 2019
09:00 AM PDT
C.E. CREDITS
SEP 24, 2019
09:00 AM PDT
DATE: September 24, 2019TIME: 9:00am PDTThis webinar will cover the basics of the Milo Single-Cell Western blot (SCW) and RNAScope single-molecule fluorescence RNA In situ hybridizati...
Speaker:
Brian P. Hermann, PhD
Sponsored By: Advanced Cell Diagnostics
SEP 05, 2019
04:00 PM CEST
C.E. CREDITS
SEP 05, 2019
04:00 PM CEST
DATE: September 5, 2019TIME: 7:00am PT, 10:00am ET, 4:00pm CEST PCR (Polymerase Chain Reaction) has gone through a massive evolution since its development in 1983. Besides it...
Speaker:
Tim Schommartz, PhD
Sponsored By: Eppendorf
JUN 05, 2019
05:00 PM CEST
C.E. CREDITS
JUN 05, 2019
05:00 PM CEST
DATE: June 5, 2019TIME: 8:00am PDT, 11:00am EDT, 5:00pm CEST Eukaryotic cell cultures respond to the most subtle influence. Apart from the risk of contamination, minimal chan...
Speaker:
Jessica Wagener, PhD
Sponsored By: Eppendorf
MAY 23, 2019
09:00 AM PDT
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MAY 23, 2019
09:00 AM PDT
DATE: May 23, 2019TIME: 9:00am PDT, 12:00pm EDT Although mesenchymal stem/stromal cells (MSCs) chondrogenic differentiation has been thoroughly investigated...
Speaker:
Melika Sarem, PhD
Sponsored By: Thermo Fisher Scientific/Gibco
MAY 16, 2019
04:00 PM CEST
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MAY 16, 2019
04:00 PM CEST
DATE: May 16, 2019TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST The emergence of NGS is revolutionizing the microbiological sciences and transforming medicine. Deep sequencing has...
Speaker:
Rita Colwell, PhD
,
Christiane Honisch, PhD
Sponsored By: Eppendorf
APR 09, 2019
09:00 AM PDT
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APR 09, 2019
09:00 AM PDT
DATE: April 9, 2019TIME: 9:00 AM PDTWe will provide a general introduction to the Single-Cell Western Technology (SCW) and then present data from 2 ongoing projects from the Col...
Speaker:
Noemi Kedei, MD
,
Kelly Gardner, Ph.D.
Sponsored By: ProteinSimple
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OCT 10, 2018 06:00 AM PDTC.E. CREDITS
OCT 10, 2018 06:00 AM PDTThe 6th Annual Cancer Research & Oncology Virtual Event will take place October 2018. The free, two-day online event will bring research professionals, scientists, and clinicians from aro...OCT 03, 2018 06:00 AM PDTC.E. CREDITS
OCT 03, 2018 06:00 AM PDTLabRoots invites you to the First Annual CRISPR Virtual Event! With continued adoption and integration in research labs, CRISPR gene editing has gained importance both as a research tool in d...SEP 17, 2018C.E. CREDITS
SEP 17, 2018Improve your protein research knowledge by joining our 7 Steps of Protein virtual eventThe 3rd annual 7 Steps of Protein virtual event is taking place around the world on Tuesday, Septemb...
Experience the latest advances in protein expression at our 3rd annual virtual event In 2017, the virtual event had 2,400 international participants in over 80 countries! Th...OCT 11, 2017 06:00 AM PDTC.E. CREDITS
OCT 11, 2017 06:00 AM PDTThe 5th Annual LabRoots Cancer Research & Oncology Virtual Conference is now On-Demand! LabRoots invites research professionals, scientists, and clinicians to this premier online co...
OCT 10, 2018
06:00 AM PDT
C.E. CREDITS
OCT 10, 2018
06:00 AM PDT
The 6th Annual Cancer Research & Oncology Virtual Event will take place October 2018. The free, two-day online event will bring research professionals, scientists, and clinicians from aro...
OCT 03, 2018
06:00 AM PDT
C.E. CREDITS
OCT 03, 2018
06:00 AM PDT
LabRoots invites you to the First Annual CRISPR Virtual Event! With continued adoption and integration in research labs, CRISPR gene editing has gained importance both as a research tool in d...
SEP 17, 2018
C.E. CREDITS
SEP 17, 2018
Improve your protein research knowledge by joining our 7 Steps of Protein virtual eventThe 3rd annual 7 Steps of Protein virtual event is taking place around the world on Tuesday, Septemb...
Experience the latest advances in protein expression at our 3rd annual virtual event In 2017, the virtual event had 2,400 international participants in over 80 countries! Th...
OCT 11, 2017
06:00 AM PDT
C.E. CREDITS
OCT 11, 2017
06:00 AM PDT
The 5th Annual LabRoots Cancer Research & Oncology Virtual Conference is now On-Demand! LabRoots invites research professionals, scientists, and clinicians to this premier online co...
APR 10, 2017
06:00 AM PDT
C.E. CREDITS
APR 10, 2017
06:00 AM PDT
The Protein and cell analysis education site is a free-access destination where you can learn more about applications and techniques related to protein gel chemistries, western detection, mas...
JAN 25, 2017
05:00 AM PST
C.E. CREDITS
JAN 25, 2017
05:00 AM PST
The Abcam virtual exhibition is temporarily closed. It will re-open shortly. Please check back October 25th. All webinars will be available for viewing at that time.Get the su...
