Western Blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used in molecular biology, immunogenetics and other molecular biology disciplines to detect specific proteins in a sample of tissue homogenate or extract. In brief, the sample undergoes protein denaturation, followed by gel electrophoresis. Synthetic or animal-derived antibodies are created that recognise and bind to a specific target protein, known as the primary antibody. The electrophoresis membrane is washed in a solution containing the primary antibody, before excess antibody is washed off. A secondary antibody is added which recognises and binds to the primary antibody. The secondary antibody is visualised through various methods such as staining, immunofluorescence, and radioactivity, allowing indirect detection of the specific target protein.
What's Popular in Western Blot
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JAN 27, 2019Neuroscience
JAN 27, 2019A study out of John's Hopkins University published by Dr. Faith Dickerson, MD, and colleagues late last year have fo ...Written By: Amy LoriauxOCT 29, 2018Neuroscience
OCT 29, 2018Undersatnding the role of gut microbiome in disease pathologies and targetting them for potential treatment strategies.Written By: Aswini Kanneganti
JAN 27, 2019
Neuroscience
JAN 27, 2019
A study out of John's Hopkins University published by Dr. Faith Dickerson, MD, and colleagues late last year have fo
...
Written By:
Amy Loriaux
OCT 29, 2018
Neuroscience
OCT 29, 2018
Undersatnding the role of gut microbiome in disease pathologies and targetting them for potential treatment strategies.
Written By:
Aswini Kanneganti
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Date: September 23, 2021 Time: 9:00am (PDT), 12:00pm (EDT) Western blotting and other immunological protein-analytical methods are widely used for detecting and (semi-)quantifying specific p...Speaker: Christian Moritz, PhDSponsored By: ProteinSimple
Date: October 05, 2021 Time: 7:00am (PDT), 10:00am (EDT) After new users become familiar with flow cytometry instrumentation and learn how to set up the instrument and acquire data, the next...Speaker: Michael Kapinsky, PhD , Matthew GoffSponsored By: Beckman Coulter Life Sciences
Date: October 12, 2021 Time: 8:00am (PDT), 11:00am (EDT) 222 Learning Objectives 222 222 222 Webinars will be available for unlimited on-demand viewing after live event. LabRoots is approved...Speaker: Jordan Thompson, PhDPresented at: Webinar series: Simple steps to publication-quality western blotsSponsored By: Thermo Fisher Scientific
Date: October 13, 2021 Time: 7:00am (PDT), 10:00am (EDT) Western blot analysis is a method widely used in the lab today because of its versatility in detecting and measuring specific target...Speaker: Caleb ShearrowPresented at: Webinar series: Simple steps to publication-quality western blotsSponsored By: Thermo Fisher Scientific
Date: October 14, 2021 Time: 10:00am (PDT), 12:00pm (EDT), 5:00pm GMT Western blot analysis is a method widely used in the lab today because of its versatility in detecting and measuring spe...Speaker: Kelli Feather-HeniganPresented at: Webinar series: Simple steps to publication-quality western blotsSponsored By: Thermo Fisher Scientific
Date: September 23, 2021 Time: 9:00am (PDT), 12:00pm (EDT) Western blotting and other immunological protein-analytical methods are widely used for detecting and (semi-)quantifying specific p...
Speaker:
Christian Moritz, PhD
Sponsored By: ProteinSimple
Date: October 05, 2021 Time: 7:00am (PDT), 10:00am (EDT) After new users become familiar with flow cytometry instrumentation and learn how to set up the instrument and acquire data, the next...
Speaker:
Michael Kapinsky, PhD
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Matthew Goff
Sponsored By: Beckman Coulter Life Sciences
Date: October 12, 2021 Time: 8:00am (PDT), 11:00am (EDT) 222 Learning Objectives 222 222 222 Webinars will be available for unlimited on-demand viewing after live event. LabRoots is approved...
Speaker:
Jordan Thompson, PhD
Presented at: Webinar series: Simple steps to publication-quality western blotsSponsored By: Thermo Fisher Scientific
Date: October 13, 2021 Time: 7:00am (PDT), 10:00am (EDT) Western blot analysis is a method widely used in the lab today because of its versatility in detecting and measuring specific target...
Speaker:
Caleb Shearrow
Presented at: Webinar series: Simple steps to publication-quality western blotsSponsored By: Thermo Fisher Scientific
Date: October 14, 2021 Time: 10:00am (PDT), 12:00pm (EDT), 5:00pm GMT Western blot analysis is a method widely used in the lab today because of its versatility in detecting and measuring spe...
Speaker:
Kelli Feather-Henigan
Presented at: Webinar series: Simple steps to publication-quality western blotsSponsored By: Thermo Fisher Scientific
JUN 09, 2021
7:00 AM PDT
C.E. CREDITS
JUN 09, 2021
7:00 AM PDT
Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...
Speaker:
Gabriela Saldanha
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Jessica Wagener, PhD
Sponsored By: Eppendorf
JUN 08, 2021
9:00 AM PDT
JUN 08, 2021
9:00 AM PDT
Date: June 8, 2021 Time: 9:00am PDT Reducing the spread of highly infectious and deadly diseases within the population, vaccine development is crucial in saving millions of lives each year....
Speaker:
Laura Chapman
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Ketil Pedersen
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Alexander "Sasha" Vlassov
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Matt Mckenna
Sponsored By: Thermo Fisher Scientific
MAY 27, 2021
8:00 AM PDT
MAY 27, 2021
8:00 AM PDT
Date: May 27, 2021 Time: 8:00am (PDT), 11:00am (EDT) With social distancing measures still enforced in large parts of the world, many researchers must minimize time in the lab where they may...
Speaker:
Chris Heger, Ph.D
Sponsored By: Bio-Techne
MAY 25, 2021
8:00 AM PDT
C.E. CREDITS
MAY 25, 2021
8:00 AM PDT
Date: May 25, 2021 Time: 8:00am (PDT) Reliable expression of recombinant proteins in human, CHO, or insect cells is essential for many aspects of biomedical research and drug development, bu...
Speaker:
Stephan Fath, PhD
Sponsored By: Thermo Fisher Scientific
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SEP 08, 2021 6:00 AM PDT
SEP 08, 2021 6:00 AM PDTLuminex's xMAP® Technology enables the evaluation of up to 500 analytes simultaneously in a single well, known as multiplexing. xMAP Technology uses color-coded microspheres as the substrate...OCT 10, 2018 6:00 AM PDT
OCT 10, 2018 6:00 AM PDTThe 6th Annual Cancer Research & Oncology Virtual Event will take place October 2018. The free, two-day online event will bring research professionals, scientists, and clinicians from aro...OCT 03, 2018 6:00 AM PDT
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Experience the latest advances in protein expression at our 3rd annual virtual event In 2017, the virtual event had 2,400 international participants in over 80 countries! Th...
SEP 08, 2021
6:00 AM PDT
SEP 08, 2021
6:00 AM PDT
Luminex's xMAP® Technology enables the evaluation of up to 500 analytes simultaneously in a single well, known as multiplexing. xMAP Technology uses color-coded microspheres as the substrate...
OCT 10, 2018
6:00 AM PDT
OCT 10, 2018
6:00 AM PDT
The 6th Annual Cancer Research & Oncology Virtual Event will take place October 2018. The free, two-day online event will bring research professionals, scientists, and clinicians from aro...
OCT 03, 2018
6:00 AM PDT
OCT 03, 2018
6:00 AM PDT
LabRoots invites you to the First Annual CRISPR Virtual Event! With continued adoption and integration in research labs, CRISPR gene editing has gained importance both as a research tool in d...
SEP 17, 2018
SEP 17, 2018
Improve your protein research knowledge by joining our 7 Steps of Protein virtual eventThe 3rd annual 7 Steps of Protein virtual event is taking place around the world on Tuesday, Septemb...
Experience the latest advances in protein expression at our 3rd annual virtual event In 2017, the virtual event had 2,400 international participants in over 80 countries! Th...
OCT 11, 2017
6:00 AM PDT
OCT 11, 2017
6:00 AM PDT
The 5th Annual LabRoots Cancer Research & Oncology Virtual Conference is now On-Demand! LabRoots invites research professionals, scientists, and clinicians to this premier online co...
APR 10, 2017
6:00 AM PDT
APR 10, 2017
6:00 AM PDT
The Protein and cell analysis education site is a free-access destination where you can learn more about applications and techniques related to protein gel chemistries, western detection, mas...
JAN 25, 2017
5:00 AM PST
JAN 25, 2017
5:00 AM PST
The Abcam virtual exhibition is temporarily closed. It will re-open shortly. Please check back October 25th. All webinars will be available for viewing at that time.Get the su...
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