Western Blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used in molecular biology, immunogenetics and other molecular biology disciplines to detect specific proteins in a sample of tissue homogenate or extract. In brief, the sample undergoes protein denaturation, followed by gel electrophoresis. Synthetic or animal-derived antibodies are created that recognise and bind to a specific target protein, known as the primary antibody. The electrophoresis membrane is washed in a solution containing the primary antibody, before excess antibody is washed off. A secondary antibody is added which recognises and binds to the primary antibody. The secondary antibody is visualised through various methods such as staining, immunofluorescence, and radioactivity, allowing indirect detection of the specific target protein.
What's Popular in Western Blot
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OCT 29, 2018Neuroscience
OCT 29, 2018Undersatnding the role of gut microbiome in disease pathologies and targetting them for potential treatment strategies....Written By: Aswini Kanneganti
OCT 29, 2018
Neuroscience
OCT 29, 2018
Undersatnding the role of gut microbiome in disease pathologies and targetting them for potential treatment strategies....
Written By:
Aswini Kanneganti
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DEC 20, 2018 09:00 AM CSTC.E. CREDITS
DEC 20, 2018 09:00 AM CSTDATE: December 20, 2018TIME: 09:00am CST, 10:00am JST Host cell protein (HCP) is a significant class of process-related impurity that needs to be removed to adequatel...Speaker: Fengqiang Wang, PhDSponsored By: GE Healthcare Life SciencesDEC 18, 2018 07:00 AM PSTC.E. CREDITS
DEC 18, 2018 07:00 AM PSTDATE: December 18,2018TIME: 07:00am PST, 10:00am EST, 15:00pm GMT Host cell protein (HCP) is a significant class of process-related impurity that needs to be removed to...Speaker: Fengqiang Wang, PhDSponsored By: GE Healthcare Life SciencesDEC 13, 2018 09:00 AM PSTC.E. CREDITS
DEC 13, 2018 09:00 AM PSTDATE: December 13 15,2018TIME: 09:00am PST, 12:00pm EST Breast cancers are classified into three main subtypes according to their receptor status: estrogen receptor-positive...Speaker: Hillary Stires, PhDSponsored By: Thermo Fisher Scientific/GibcoOCT 11, 2018 01:30 PM PDTC.E. CREDITS
OCT 11, 2018 01:30 PM PDTAs the most common female malignancy, breast cancer is the most likely reason that a woman will die of cancer around the world. Breast cancer mortality has dropped in the U.S. by 35% since 19...Speaker: Benjamin Anderson, MDPresented At:Cancer Research & Oncology 2018OCT 11, 2018 12:00 PM PDTC.E. CREDITS
OCT 11, 2018 12:00 PM PDTLung cancer is the leading cause of cancer-related mortality worldwide. Large-scale sequencing studies have revealed the complex genomic landscape of NSCLC and genomic differences between lun...Speaker: Nicholas McGranahan, PhDPresented At:Cancer Research & Oncology 2018
DEC 20, 2018
09:00 AM CST
C.E. CREDITS
DEC 20, 2018
09:00 AM CST
DATE: December 20, 2018TIME: 09:00am CST, 10:00am JST Host cell protein (HCP) is a significant class of process-related impurity that needs to be removed to adequatel...
Speaker:
Fengqiang Wang, PhD
Sponsored By: GE Healthcare Life Sciences
DEC 18, 2018
07:00 AM PST
C.E. CREDITS
DEC 18, 2018
07:00 AM PST
DATE: December 18,2018TIME: 07:00am PST, 10:00am EST, 15:00pm GMT Host cell protein (HCP) is a significant class of process-related impurity that needs to be removed to...
Speaker:
Fengqiang Wang, PhD
Sponsored By: GE Healthcare Life Sciences
DEC 13, 2018
09:00 AM PST
C.E. CREDITS
DEC 13, 2018
09:00 AM PST
DATE: December 13 15,2018TIME: 09:00am PST, 12:00pm EST Breast cancers are classified into three main subtypes according to their receptor status: estrogen receptor-positive...
Speaker:
Hillary Stires, PhD
Sponsored By: Thermo Fisher Scientific/Gibco
OCT 11, 2018
01:30 PM PDT
C.E. CREDITS
OCT 11, 2018
01:30 PM PDT
As the most common female malignancy, breast cancer is the most likely reason that a woman will die of cancer around the world. Breast cancer mortality has dropped in the U.S. by 35% since 19...
Speaker:
Benjamin Anderson, MD
Presented At:Cancer Research & Oncology 2018
OCT 11, 2018
12:00 PM PDT
C.E. CREDITS
OCT 11, 2018
12:00 PM PDT
Lung cancer is the leading cause of cancer-related mortality worldwide. Large-scale sequencing studies have revealed the complex genomic landscape of NSCLC and genomic differences between lun...
Speaker:
Nicholas McGranahan, PhD
Presented At:Cancer Research & Oncology 2018
OCT 11, 2018
10:30 AM PDT
C.E. CREDITS
OCT 11, 2018
10:30 AM PDT
Two projects looking at novel approaches to targeting inflammatory breast cancer will be presented. Inflammatory breast cancer (IBC) is a unique, understudied, and most lethal subtype account...
Speaker:
Kevin Williams, PhD
Presented At:Cancer Research & Oncology 2018
OCT 11, 2018
09:00 AM PDT
C.E. CREDITS
OCT 11, 2018
09:00 AM PDT
The oncogenic transcription factor c-MYC (MYC) is deregulated, and often overexpressed, in more than 50% of cancers. MYC deregulation is associated with poor prognosis and aggressive disease,...
Speaker:
Jason De Melo, PhD
Presented At:Cancer Research & Oncology 2018
OCT 11, 2018
07:30 AM PDT
C.E. CREDITS
OCT 11, 2018
07:30 AM PDT
In the past two decades a small number of infrequently dividing cells have been proposed as the source of multi-drug resistance during cancer treatment. These cells identified by their expres...
Speaker:
Krastan Blagoev, PhD
Presented At:Cancer Research & Oncology 2018
OCT 11, 2018
06:00 AM PDT
C.E. CREDITS
OCT 11, 2018
06:00 AM PDT
PacBio Sequencing simultaneously provides long sequence reads, high consensus accuracy, minimal sequence bias, and methylation detection. I will highlight new advances and updates on applying...
Speaker:
Jonas Korlach, PhD
Presented At:Cancer Research & Oncology 2018
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OCT 10, 2018 06:00 AM PDTC.E. CREDITS
OCT 10, 2018 06:00 AM PDTThe 6th Annual Cancer Research & Oncology Virtual Event will take place October 2018. The free, two-day online event will bring research professionals, scientists, and clinicians from aro...OCT 03, 2018 06:00 AM PDTC.E. CREDITS
OCT 03, 2018 06:00 AM PDTLabRoots invites you to the First Annual CRISPR Virtual Event! With continued adoption and integration in research labs, CRISPR gene editing has gained importance both as a research tool in d...SEP 17, 2018C.E. CREDITS
SEP 17, 2018Improve your protein research knowledge by joining our 7 Steps of Protein virtual eventThe 3rd annual 7 Steps of Protein virtual event is taking place around the world on Tuesday, Septemb...
Experience the latest advances in protein expression at our 3rd annual virtual event In 2017, the virtual event had 2,400 international participants in over 80 countries! Th...OCT 11, 2017 06:00 AM PDTC.E. CREDITS
OCT 11, 2017 06:00 AM PDTThe 5th Annual LabRoots Cancer Research & Oncology Virtual Conference is now On-Demand! LabRoots invites research professionals, scientists, and clinicians to this premier online co...
OCT 10, 2018
06:00 AM PDT
C.E. CREDITS
OCT 10, 2018
06:00 AM PDT
The 6th Annual Cancer Research & Oncology Virtual Event will take place October 2018. The free, two-day online event will bring research professionals, scientists, and clinicians from aro...
OCT 03, 2018
06:00 AM PDT
C.E. CREDITS
OCT 03, 2018
06:00 AM PDT
LabRoots invites you to the First Annual CRISPR Virtual Event! With continued adoption and integration in research labs, CRISPR gene editing has gained importance both as a research tool in d...
SEP 17, 2018
C.E. CREDITS
SEP 17, 2018
Improve your protein research knowledge by joining our 7 Steps of Protein virtual eventThe 3rd annual 7 Steps of Protein virtual event is taking place around the world on Tuesday, Septemb...
Experience the latest advances in protein expression at our 3rd annual virtual event In 2017, the virtual event had 2,400 international participants in over 80 countries! Th...
OCT 11, 2017
06:00 AM PDT
C.E. CREDITS
OCT 11, 2017
06:00 AM PDT
The 5th Annual LabRoots Cancer Research & Oncology Virtual Conference is now On-Demand! LabRoots invites research professionals, scientists, and clinicians to this premier online co...
APR 10, 2017
06:00 AM PDT
C.E. CREDITS
APR 10, 2017
06:00 AM PDT
The Protein and cell analysis education site is a free-access destination where you can learn more about applications and techniques related to protein gel chemistries, western detection, mas...
JAN 25, 2017
05:00 AM PST
C.E. CREDITS
JAN 25, 2017
05:00 AM PST
The Abcam virtual exhibition is temporarily closed. It will re-open shortly. Please check back October 25th. All webinars will be available for viewing at that time.Get the su...
