Western Blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used in molecular biology, immunogenetics and other molecular biology disciplines to detect specific proteins in a sample of tissue homogenate or extract. In brief, the sample undergoes protein denaturation, followed by gel electrophoresis. Synthetic or animal-derived antibodies are created that recognise and bind to a specific target protein, known as the primary antibody. The electrophoresis membrane is washed in a solution containing the primary antibody, before excess antibody is washed off. A secondary antibody is added which recognises and binds to the primary antibody. The secondary antibody is visualised through various methods such as staining, immunofluorescence, and radioactivity, allowing indirect detection of the specific target protein.
What's Popular in Western Blot
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JUL 14, 2022Clinical & Molecular DX
JUL 14, 2022Magnetic beads (or superparamagnetic particles) can be used for the isolation and purification of cells, nucleic acids, ...Sponsored By: CytivaFEB 11, 2022Cannabis Sciences
FEB 11, 2022According to a recent study published in Oncotarget, cannabis extracts may harbor the potential to eventually be tailore ...Written By: Nadine HusamiDEC 01, 2021Plants & Animals
DEC 01, 2021Along with milk and peanut allergies, egg allergies are among the most common food allergies in the world. It’s es ...Written By: Ryan VingumJAN 27, 2019Neuroscience
JAN 27, 2019A study out of John's Hopkins University published by Dr. Faith Dickerson, MD, and colleagues late last year have fo ...Written By: Amy LoriauxOCT 29, 2018Neuroscience
OCT 29, 2018Undersatnding the role of gut microbiome in disease pathologies and targetting them for potential treatment strategies.Written By: Aswini Kanneganti
JUL 14, 2022
Clinical & Molecular DX
JUL 14, 2022
Magnetic beads (or superparamagnetic particles) can be used for the isolation and purification of cells, nucleic acids,
...
Sponsored By:
Cytiva
FEB 11, 2022
Cannabis Sciences
FEB 11, 2022
According to a recent study published in Oncotarget, cannabis extracts may harbor the potential to eventually be tailore
...
Written By:
Nadine Husami
DEC 01, 2021
Plants & Animals
DEC 01, 2021
Along with milk and peanut allergies, egg allergies are among the most common food allergies in the world. It’s es
...
Written By:
Ryan Vingum
JAN 27, 2019
Neuroscience
JAN 27, 2019
A study out of John's Hopkins University published by Dr. Faith Dickerson, MD, and colleagues late last year have fo
...
Written By:
Amy Loriaux
OCT 29, 2018
Neuroscience
OCT 29, 2018
Undersatnding the role of gut microbiome in disease pathologies and targetting them for potential treatment strategies.
Written By:
Aswini Kanneganti
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OCT 14, 2021 9:00 AM PDTC.E. CREDITS
OCT 14, 2021 9:00 AM PDTDate: October 14, 2021 Time: 9:00am (PDT), 12:00pm (EDT), 5:00pm GMT Western blot analysis is a method widely used in the lab today because of its versatility in detecting and measuring spe...Speaker: Kelli Feather-HeniganPresented at: Webinar series: Simple steps to publication-quality western blotsSponsored By: Thermo Fisher ScientificOCT 13, 2021 3:00 PM PDTC.E. CREDITS
OCT 13, 2021 3:00 PM PDTSARS-CoV-2 testing is now facing increasing challenges in the face of an ongoing pandemic with the emergence of new variants and waning vaccine immunity. Increasing evidence supports the cor...Speaker: Sean Taylor, MBA, PhDPresented at: Coronavirus Virtual Event SeriesSponsored By: GenScript
OCT 13, 2021
7:00 AM PDT
C.E. CREDITS
OCT 13, 2021
7:00 AM PDT
Date: October 13, 2021 Time: 7:00am (PDT), 10:00am (EDT) Western blot analysis is a method widely used in the lab today because of its versatility in detecting and measuring specific target...
Speaker:
Caleb Shearrow
Presented at: Webinar series: Simple steps to publication-quality western blotsSponsored By: Thermo Fisher Scientific
OCT 12, 2021
5:00 PM PDT
C.E. CREDITS
OCT 12, 2021
5:00 PM PDT
Date: October 12, 2021 Time: 5:00pm (PDT), 8:00pm (EDT) 222 Learning Objectives 222 222 222 Webinars will be available for unlimited on-demand viewing after live event. LabRoots is approved...
Speaker:
Jordan Thompson, PhD
Presented at: Webinar series: Simple steps to publication-quality western blotsSponsored By: Thermo Fisher Scientific
OCT 05, 2021
7:00 AM PDT
C.E. CREDITS
OCT 05, 2021
7:00 AM PDT
Date: October 05, 2021 Time: 7:00am (PDT), 10:00am (EDT) After new users become familiar with flow cytometry instrumentation and learn how to set up the instrument and acquire data, the next...
Speaker:
Michael Kapinsky, PhD
,
Matthew Goff
Sponsored By: Beckman Coulter Life Sciences
OCT 14, 2021
9:00 AM PDT
C.E. CREDITS
OCT 14, 2021
9:00 AM PDT
Date: October 14, 2021 Time: 9:00am (PDT), 12:00pm (EDT), 5:00pm GMT Western blot analysis is a method widely used in the lab today because of its versatility in detecting and measuring spe...
Speaker:
Kelli Feather-Henigan
Presented at: Webinar series: Simple steps to publication-quality western blotsSponsored By: Thermo Fisher Scientific
OCT 13, 2021
3:00 PM PDT
C.E. CREDITS
OCT 13, 2021
3:00 PM PDT
SARS-CoV-2 testing is now facing increasing challenges in the face of an ongoing pandemic with the emergence of new variants and waning vaccine immunity. Increasing evidence supports the cor...
Speaker:
Sean Taylor, MBA, PhD
Presented at: Coronavirus Virtual Event SeriesSponsored By: GenScript
OCT 13, 2021
7:00 AM PDT
C.E. CREDITS
OCT 13, 2021
7:00 AM PDT
Date: October 13, 2021 Time: 7:00am (PDT), 10:00am (EDT) Western blot analysis is a method widely used in the lab today because of its versatility in detecting and measuring specific target...
Speaker:
Caleb Shearrow
Presented at: Webinar series: Simple steps to publication-quality western blotsSponsored By: Thermo Fisher Scientific
OCT 12, 2021
5:00 PM PDT
C.E. CREDITS
OCT 12, 2021
5:00 PM PDT
Date: October 12, 2021 Time: 5:00pm (PDT), 8:00pm (EDT) 222 Learning Objectives 222 222 222 Webinars will be available for unlimited on-demand viewing after live event. LabRoots is approved...
Speaker:
Jordan Thompson, PhD
Presented at: Webinar series: Simple steps to publication-quality western blotsSponsored By: Thermo Fisher Scientific
OCT 05, 2021
7:00 AM PDT
C.E. CREDITS
OCT 05, 2021
7:00 AM PDT
Date: October 05, 2021 Time: 7:00am (PDT), 10:00am (EDT) After new users become familiar with flow cytometry instrumentation and learn how to set up the instrument and acquire data, the next...
Speaker:
Michael Kapinsky, PhD
,
Matthew Goff
Sponsored By: Beckman Coulter Life Sciences
SEP 23, 2021
9:00 AM PDT
C.E. CREDITS
SEP 23, 2021
9:00 AM PDT
Date: September 23, 2021 Time: 9:00am (PDT), 12:00pm (EDT) Western blotting and other immunological protein-analytical methods are widely used for detecting and (semi-)quantifying specific p...
Speaker:
Christian Moritz, PhD
Sponsored By: ProteinSimple
SEP 22, 2021
12:00 PM PDT
C.E. CREDITS
SEP 22, 2021
12:00 PM PDT
While providing indispensable insight into immune processes, the live cell imaging of immune cells poses unique challenges due to their non-adherent nature. Suspension cells such as immune c...
Speaker:
Jun Park, PhD
Presented at: Cell Biology 2021Sponsored By: MilliporeSigma
JUN 09, 2021
7:00 AM PDT
C.E. CREDITS
JUN 09, 2021
7:00 AM PDT
Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...
Speaker:
Gabriela Saldanha
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Jessica Wagener, PhD
Sponsored By: Eppendorf
JUN 08, 2021
9:00 AM PDT
JUN 08, 2021
9:00 AM PDT
Date: June 8, 2021 Time: 9:00am PDT Reducing the spread of highly infectious and deadly diseases within the population, vaccine development is crucial in saving millions of lives each year....
Speaker:
Laura Chapman
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Ketil Pedersen, Ph.D
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Alexander "Sasha" Vlassov
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Matt Mckenna
Sponsored By: Thermo Fisher Scientific
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SEP 08, 2021 6:00 AM PDT
SEP 08, 2021 6:00 AM PDTLuminex's xMAP® Technology enables the evaluation of up to 500 analytes simultaneously in a single well, known as multiplexing. xMAP Technology uses color-coded microspheres as the substrate...C.E. CREDITS
Western blot analysis is a method widely used in the lab today because of its versatility in detecting and measuring specific target proteins in a sample. However, in recent years the reprod...OCT 10, 2018 6:00 AM PDT
OCT 10, 2018 6:00 AM PDTThe 6th Annual Cancer Research & Oncology Virtual Event will take place October 2018. The free, two-day online event will bring research professionals, scientists, and clinicians from aro...OCT 03, 2018 6:00 AM PDT
OCT 03, 2018 6:00 AM PDTLabRoots invites you to the First Annual CRISPR Virtual Event! With continued adoption and integration in research labs, CRISPR gene editing has gained importance both as a research tool in d...SEP 17, 2018
SEP 17, 2018Improve your protein research knowledge by joining our 7 Steps of Protein virtual eventThe 3rd annual 7 Steps of Protein virtual event is taking place around the world on Tuesday, Septemb...
SEP 08, 2021
6:00 AM PDT
SEP 08, 2021
6:00 AM PDT
Luminex's xMAP® Technology enables the evaluation of up to 500 analytes simultaneously in a single well, known as multiplexing. xMAP Technology uses color-coded microspheres as the substrate...
C.E. CREDITS
Western blot analysis is a method widely used in the lab today because of its versatility in detecting and measuring specific target proteins in a sample. However, in recent years the reprod...
OCT 10, 2018
6:00 AM PDT
OCT 10, 2018
6:00 AM PDT
The 6th Annual Cancer Research & Oncology Virtual Event will take place October 2018. The free, two-day online event will bring research professionals, scientists, and clinicians from aro...
OCT 03, 2018
6:00 AM PDT
OCT 03, 2018
6:00 AM PDT
LabRoots invites you to the First Annual CRISPR Virtual Event! With continued adoption and integration in research labs, CRISPR gene editing has gained importance both as a research tool in d...
SEP 17, 2018
SEP 17, 2018
Improve your protein research knowledge by joining our 7 Steps of Protein virtual eventThe 3rd annual 7 Steps of Protein virtual event is taking place around the world on Tuesday, Septemb...
Experience the latest advances in protein expression at our 3rd annual virtual event In 2017, the virtual event had 2,400 international participants in over 80 countries! Th...
OCT 11, 2017
6:00 AM PDT
OCT 11, 2017
6:00 AM PDT
The 5th Annual LabRoots Cancer Research & Oncology Virtual Conference is now On-Demand! LabRoots invites research professionals, scientists, and clinicians to this premier online co...
APR 10, 2017
6:00 AM PDT
APR 10, 2017
6:00 AM PDT
The Protein and cell analysis education site is a free-access destination where you can learn more about applications and techniques related to protein gel chemistries, western detection, mas...
JAN 25, 2017
5:00 AM PST
JAN 25, 2017
5:00 AM PST
The Abcam virtual exhibition is temporarily closed. It will re-open shortly. Please check back October 25th. All webinars will be available for viewing at that time.Get the su...