Western Blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used in molecular biology, immunogenetics and other molecular biology disciplines to detect specific proteins in a sample of tissue homogenate or extract. In brief, the sample undergoes protein denaturation, followed by gel electrophoresis. Synthetic or animal-derived antibodies are created that recognise and bind to a specific target protein, known as the primary antibody. The electrophoresis membrane is washed in a solution containing the primary antibody, before excess antibody is washed off. A secondary antibody is added which recognises and binds to the primary antibody. The secondary antibody is visualised through various methods such as staining, immunofluorescence, and radioactivity, allowing indirect detection of the specific target protein.
What's Popular in Western Blot
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JAN 27, 2019Neuroscience
JAN 27, 2019A study out of John's Hopkins University published by Dr. Faith Dickerson, MD, and colleagues late last year have fo ...Written By: Amy LoriauxOCT 29, 2018Neuroscience
OCT 29, 2018Undersatnding the role of gut microbiome in disease pathologies and targetting them for potential treatment strategies.Written By: Aswini Kanneganti
JAN 27, 2019
Neuroscience
JAN 27, 2019
A study out of John's Hopkins University published by Dr. Faith Dickerson, MD, and colleagues late last year have fo
...
Written By:
Amy Loriaux
OCT 29, 2018
Neuroscience
OCT 29, 2018
Undersatnding the role of gut microbiome in disease pathologies and targetting them for potential treatment strategies.
Written By:
Aswini Kanneganti
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MAY 11, 2021 10:00 AM PDTAdd to Calendar Select one of the following:
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MAY 11, 2021 10:00 AM PDTAdd to Calendar Select one of the following:
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Date: May 11, 2021 Time: 10:00zm PDT Your samples are some of the most valuable assets in the laboratory. After spending countless hours on extraction and preparation, your conclusions could...Speaker: Paulina Kocjan , Kate Meola , Zareh ZurabyanPresented at: Eppendorf Planet of Knowledge - A New DimensionSponsored By: EppendorfMAY 13, 2021 11:00 AM PDTAdd to Calendar Select one of the following:
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Date: May 13, 2021 Time: 11:00am PST Dr. Dattwyler will share his extensive experience on the clinical diagnosis and treatment of Lyme disease including a brief history of Lyme Disease and h...Speaker: Raymond Dattwyler, M.D.Sponsored By: DiaSorin Inc.MAY 25, 2021 8:00 AM PDTAdd to Calendar Select one of the following:
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Date: May 25, 2021 Time: 8:00am (PDT) Reliable expression of recombinant proteins in human, CHO, or insect cells is essential for many aspects of biomedical research and drug development, bu...Speaker: Stephen Fath, PhDSponsored By: Thermo Fisher ScientificJUN 09, 2021 7:00 AM PDTAdd to Calendar Select one of the following:
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Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...Speaker: Gabriela Saldanha , Jessica Wagener, PhDSponsored By: EppendorfAPR 06, 2021 10:00 AM PDT
APR 06, 2021 10:00 AM PDTDATE: April 6, 2021 TIME: 10:00am PDT Accurate and efficient data analysis of protein targets is an integral part of the gel and western blot imaging workflow. The Invitrogen iBright Analysi...Speaker: Janaki Narahari, Ph.D.Sponsored By: Thermo Fisher Scientific
MAY 11, 2021
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Date: May 11, 2021 Time: 10:00zm PDT Your samples are some of the most valuable assets in the laboratory. After spending countless hours on extraction and preparation, your conclusions could...
Speaker:
Paulina Kocjan
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Kate Meola
,
Zareh Zurabyan
Presented at: Eppendorf Planet of Knowledge - A New DimensionSponsored By: Eppendorf
MAY 13, 2021
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MAY 13, 2021
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Date: May 13, 2021 Time: 11:00am PST Dr. Dattwyler will share his extensive experience on the clinical diagnosis and treatment of Lyme disease including a brief history of Lyme Disease and h...
Speaker:
Raymond Dattwyler, M.D.
Sponsored By: DiaSorin Inc.
MAY 25, 2021
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Date: May 25, 2021 Time: 8:00am (PDT) Reliable expression of recombinant proteins in human, CHO, or insect cells is essential for many aspects of biomedical research and drug development, bu...
Speaker:
Stephen Fath, PhD
Sponsored By: Thermo Fisher Scientific
JUN 09, 2021
7:00 AM PDT
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Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...
Speaker:
Gabriela Saldanha
,
Jessica Wagener, PhD
Sponsored By: Eppendorf
APR 06, 2021
10:00 AM PDT
APR 06, 2021
10:00 AM PDT
DATE: April 6, 2021 TIME: 10:00am PDT Accurate and efficient data analysis of protein targets is an integral part of the gel and western blot imaging workflow. The Invitrogen iBright Analysi...
Speaker:
Janaki Narahari, Ph.D.
Sponsored By: Thermo Fisher Scientific
MAR 31, 2021
8:00 AM PDT
MAR 31, 2021
8:00 AM PDT
Date: March 31, 2021 Time: 08:00am PST CytoFLEX SRT has enabled researcher lead self-service sorting. The need for a cell sorting expert to setup and maintain the sorter status is now necess...
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Peter O'Toole, PhD
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Dr. Karen Hogg
Sponsored By: Beckman Coulter Life Sciences
FEB 24, 2021
10:00 AM PST
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FEB 24, 2021
10:00 AM PST
DATE: February 24, 2021 TIME: 10am PST Automated lab instruments such as liquid handlers and cell sorters are increasingly common in all types of laboratories, driving fast results for labor...
Speaker:
David Wasescha
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Simon Fogarty
Sponsored By: Labconco
OCT 08, 2020
7:00 AM PDT
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OCT 08, 2020
7:00 AM PDT
DATE: October 8, 2020 TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop thinking about ho...
Speaker:
Nadine Mellies, PhD
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Jessica Wagener, PhD
Sponsored By: Eppendorf
OCT 08, 2020
6:00 AM PDT
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OCT 08, 2020
6:00 AM PDT
Every patient is different and so is every tumor! The term “personalized oncology” today is largely associated with sequencing approaches. Current publications proof more and mor...
Speaker:
Christian Regenbrecht, PhD
Presented at: Cancer Research & Oncology 2020Sponsored By: LUMINEX
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APR 29, 2020 6:00 AM PDT
APR 29, 2020 6:00 AM PDTLuminex's xMAP® Technology enables the evaluation of up to 500 analytes simultaneously in a single well, known as multiplexing. xMAP Technology uses color-coded microspheres as the substrate...OCT 10, 2018 6:00 AM PDT
OCT 10, 2018 6:00 AM PDTThe 6th Annual Cancer Research & Oncology Virtual Event will take place October 2018. The free, two-day online event will bring research professionals, scientists, and clinicians from aro...OCT 03, 2018 6:00 AM PDT
OCT 03, 2018 6:00 AM PDTLabRoots invites you to the First Annual CRISPR Virtual Event! With continued adoption and integration in research labs, CRISPR gene editing has gained importance both as a research tool in d...SEP 17, 2018
SEP 17, 2018Improve your protein research knowledge by joining our 7 Steps of Protein virtual eventThe 3rd annual 7 Steps of Protein virtual event is taking place around the world on Tuesday, Septemb...
Experience the latest advances in protein expression at our 3rd annual virtual event In 2017, the virtual event had 2,400 international participants in over 80 countries! Th...
APR 29, 2020
6:00 AM PDT
APR 29, 2020
6:00 AM PDT
Luminex's xMAP® Technology enables the evaluation of up to 500 analytes simultaneously in a single well, known as multiplexing. xMAP Technology uses color-coded microspheres as the substrate...
OCT 10, 2018
6:00 AM PDT
OCT 10, 2018
6:00 AM PDT
The 6th Annual Cancer Research & Oncology Virtual Event will take place October 2018. The free, two-day online event will bring research professionals, scientists, and clinicians from aro...
OCT 03, 2018
6:00 AM PDT
OCT 03, 2018
6:00 AM PDT
LabRoots invites you to the First Annual CRISPR Virtual Event! With continued adoption and integration in research labs, CRISPR gene editing has gained importance both as a research tool in d...
SEP 17, 2018
SEP 17, 2018
Improve your protein research knowledge by joining our 7 Steps of Protein virtual eventThe 3rd annual 7 Steps of Protein virtual event is taking place around the world on Tuesday, Septemb...
Experience the latest advances in protein expression at our 3rd annual virtual event In 2017, the virtual event had 2,400 international participants in over 80 countries! Th...
OCT 11, 2017
6:00 AM PDT
OCT 11, 2017
6:00 AM PDT
The 5th Annual LabRoots Cancer Research & Oncology Virtual Conference is now On-Demand! LabRoots invites research professionals, scientists, and clinicians to this premier online co...
APR 10, 2017
6:00 AM PDT
APR 10, 2017
6:00 AM PDT
The Protein and cell analysis education site is a free-access destination where you can learn more about applications and techniques related to protein gel chemistries, western detection, mas...
JAN 25, 2017
5:00 AM PST
JAN 25, 2017
5:00 AM PST
The Abcam virtual exhibition is temporarily closed. It will re-open shortly. Please check back October 25th. All webinars will be available for viewing at that time.Get the su...
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