Western Blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used in molecular biology, immunogenetics and other molecular biology disciplines to detect specific proteins in a sample of tissue homogenate or extract. In brief, the sample undergoes protein denaturation, followed by gel electrophoresis. Synthetic or animal-derived antibodies are created that recognise and bind to a specific target protein, known as the primary antibody. The electrophoresis membrane is washed in a solution containing the primary antibody, before excess antibody is washed off. A secondary antibody is added which recognises and binds to the primary antibody. The secondary antibody is visualised through various methods such as staining, immunofluorescence, and radioactivity, allowing indirect detection of the specific target protein.
What's Popular in Western Blot
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JAN 27, 2019Neuroscience
JAN 27, 2019A study out of John's Hopkins University published by Dr. Faith Dickerson, MD, and colleagues late last year have fo ...WRITTEN BY: Amy LoriauxOCT 29, 2018Neuroscience
OCT 29, 2018Undersatnding the role of gut microbiome in disease pathologies and targetting them for potential treatment strategies.WRITTEN BY: Aswini Kanneganti
JAN 27, 2019
Neuroscience
JAN 27, 2019
A study out of John's Hopkins University published by Dr. Faith Dickerson, MD, and colleagues late last year have fo
...
WRITTEN BY:
Amy Loriaux
OCT 29, 2018
Neuroscience
OCT 29, 2018
Undersatnding the role of gut microbiome in disease pathologies and targetting them for potential treatment strategies.
WRITTEN BY:
Aswini Kanneganti
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JUN 18, 2020 9:00 AM PDTAdd to Calendar Select one of the following:
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JUN 18, 2020 9:00 AM PDTAdd to Calendar Select one of the following:
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DATE: June 18, 2020 TIME: 9:00am PDT, 12:00pm EDT Despite the success of immunotherapy against several malignancies including melanoma and lung adenocarcinoma, glioblastoma remains the excep...Speaker: Felipe Galvez Cancino, Ph.D.Sponsored By: Thermo Fisher Scientific/GibcoAPR 28, 2020 10:00 AM PDTC.E. CREDITS
APR 28, 2020 10:00 AM PDTDATE: April 28, 2020 TIME: 10:00am PT All cells release extracellular vesicles (EVs) that can carry molecular cargo to other cells to affect their function. EVs are promising as potential bi...Speaker: John Nolan, PhDSponsored By: Beckman Coulter Life SciencesAPR 14, 2020 9:00 AM PDT
APR 14, 2020 9:00 AM PDTDATE: April 14, 2020 TIME: 9:00am PDT, 12:00pm EDT Besides being Gibco Cell Culture Heroes, what do Vivek, Sandra, Daisy, Ameet and Kristine all have in common? They are all stuck at home fo...Speaker: Kristine Wadosky, PhD , Ameet Chimote, PhD , Elizabeth Delery, PhD , Daisy Shu, B.Optom, PhD , Vivek Kamat, PhD , Sandra Hammer, PhDSponsored By: Thermo Fisher Scientific/GibcoMAR 27, 2020 9:00 AM PDTC.E. CREDITS
MAR 27, 2020 9:00 AM PDTDATE: March 27, 2020 TIME: 9:00am PDT, 12:00pm EDT Osteoporosis causes a decrease in bone density, along with deterioration of the bone’s microarchitecture at a faster rate than normal...Speaker: Mayda ArshadSponsored By: Thermo Fisher Scientific/GibcoJAN 30, 2020 9:00 AM PSTC.E. CREDITS
JAN 30, 2020 9:00 AM PSTDATE: January 30, 2020 TIME: 9:00am PST, 12:00pm EST Recent advances in in vitro 3D cellular culture technologies, such as organoids, rapidly developed and established novel, more physiologi...Speaker: Brooke SchusterSponsored By: Thermo Fisher Scientific/Gibco
JUN 18, 2020
9:00 AM PDT
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JUN 18, 2020
9:00 AM PDT
Add to Calendar
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DATE: June 18, 2020 TIME: 9:00am PDT, 12:00pm EDT Despite the success of immunotherapy against several malignancies including melanoma and lung adenocarcinoma, glioblastoma remains the excep...
Speaker:
Felipe Galvez Cancino, Ph.D.
Sponsored By: Thermo Fisher Scientific/Gibco
APR 28, 2020
10:00 AM PDT
C.E. CREDITS
APR 28, 2020
10:00 AM PDT
DATE: April 28, 2020 TIME: 10:00am PT All cells release extracellular vesicles (EVs) that can carry molecular cargo to other cells to affect their function. EVs are promising as potential bi...
Speaker:
John Nolan, PhD
Sponsored By: Beckman Coulter Life Sciences
APR 14, 2020
9:00 AM PDT
APR 14, 2020
9:00 AM PDT
DATE: April 14, 2020 TIME: 9:00am PDT, 12:00pm EDT Besides being Gibco Cell Culture Heroes, what do Vivek, Sandra, Daisy, Ameet and Kristine all have in common? They are all stuck at home fo...
Speaker:
Kristine Wadosky, PhD
,
Ameet Chimote, PhD
,
Elizabeth Delery, PhD
,
Daisy Shu, B.Optom, PhD
,
Vivek Kamat, PhD
,
Sandra Hammer, PhD
Sponsored By: Thermo Fisher Scientific/Gibco
MAR 27, 2020
9:00 AM PDT
C.E. CREDITS
MAR 27, 2020
9:00 AM PDT
DATE: March 27, 2020 TIME: 9:00am PDT, 12:00pm EDT Osteoporosis causes a decrease in bone density, along with deterioration of the bone’s microarchitecture at a faster rate than normal...
Speaker:
Mayda Arshad
Sponsored By: Thermo Fisher Scientific/Gibco
JAN 30, 2020
9:00 AM PST
C.E. CREDITS
JAN 30, 2020
9:00 AM PST
DATE: January 30, 2020 TIME: 9:00am PST, 12:00pm EST Recent advances in in vitro 3D cellular culture technologies, such as organoids, rapidly developed and established novel, more physiologi...
Speaker:
Brooke Schuster
Sponsored By: Thermo Fisher Scientific/Gibco
DEC 17, 2019
9:00 AM PST
C.E. CREDITS
DEC 17, 2019
9:00 AM PST
DATE: December 17, 2019TIME: 9:00am PST, 12:00pm EST Purpose: Liver x receptors (LXRs) are hypothesized to serve as a link between lipid metabolism and inflammation by prom...
Speaker:
Sandra Hammer, PhD
Sponsored By: Thermo Fisher Scientific/Gibco
DEC 04, 2019
7:00 AM PST
C.E. CREDITS
DEC 04, 2019
7:00 AM PST
DATE: December 4, 2019TIME: 7:00am PST, 10:00am EST, 4:00pm CET Do you know how it feels when you just quickly want to redo an experiment that your colleague did, or an experim...
Speaker:
Katja Baesler, PhD
,
Ulrike Gast, PhD
,
Alisa Gruschka
Sponsored By: Eppendorf
NOV 18, 2019
7:00 AM PST
C.E. CREDITS
NOV 18, 2019
7:00 AM PST
DATE: November 18, 2019TIME: 7:00am PST, 11:00am EST, 4:00pm CEWT How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop th...
Speaker:
Nadine Mellies, PhD
,
Katja Baesler, PhD
Sponsored By: Eppendorf
NOV 05, 2019
7:00 AM PST
C.E. CREDITS
NOV 05, 2019
7:00 AM PST
DATE: November 5, 2019 TIME: 7:00am PST, 9:00am CSTDetection of low-abundance proteins and/or detection of proteins from precious samples can be a major challenge when performing wes...
Speaker:
Emily Halbrader
Sponsored By: Thermo Fisher Scientific
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APR 29, 2020 6:00 AM PDT
APR 29, 2020 6:00 AM PDTLuminex's xMAP® Technology enables the evaluation of up to 500 analytes simultaneously in a single well, known as multiplexing. xMAP Technology uses color-coded microspheres as the substrate...OCT 10, 2018 6:00 AM PDTC.E. CREDITS
OCT 10, 2018 6:00 AM PDTThe 6th Annual Cancer Research & Oncology Virtual Event will take place October 2018. The free, two-day online event will bring research professionals, scientists, and clinicians from aro...OCT 03, 2018 6:00 AM PDTC.E. CREDITS
OCT 03, 2018 6:00 AM PDTLabRoots invites you to the First Annual CRISPR Virtual Event! With continued adoption and integration in research labs, CRISPR gene editing has gained importance both as a research tool in d...SEP 17, 2018C.E. CREDITS
SEP 17, 2018Improve your protein research knowledge by joining our 7 Steps of Protein virtual eventThe 3rd annual 7 Steps of Protein virtual event is taking place around the world on Tuesday, Septemb...
Experience the latest advances in protein expression at our 3rd annual virtual event In 2017, the virtual event had 2,400 international participants in over 80 countries! Th...
APR 29, 2020
6:00 AM PDT
APR 29, 2020
6:00 AM PDT
Luminex's xMAP® Technology enables the evaluation of up to 500 analytes simultaneously in a single well, known as multiplexing. xMAP Technology uses color-coded microspheres as the substrate...
OCT 10, 2018
6:00 AM PDT
C.E. CREDITS
OCT 10, 2018
6:00 AM PDT
The 6th Annual Cancer Research & Oncology Virtual Event will take place October 2018. The free, two-day online event will bring research professionals, scientists, and clinicians from aro...
OCT 03, 2018
6:00 AM PDT
C.E. CREDITS
OCT 03, 2018
6:00 AM PDT
LabRoots invites you to the First Annual CRISPR Virtual Event! With continued adoption and integration in research labs, CRISPR gene editing has gained importance both as a research tool in d...
SEP 17, 2018
C.E. CREDITS
SEP 17, 2018
Improve your protein research knowledge by joining our 7 Steps of Protein virtual eventThe 3rd annual 7 Steps of Protein virtual event is taking place around the world on Tuesday, Septemb...
Experience the latest advances in protein expression at our 3rd annual virtual event In 2017, the virtual event had 2,400 international participants in over 80 countries! Th...
OCT 11, 2017
6:00 AM PDT
OCT 11, 2017
6:00 AM PDT
The 5th Annual LabRoots Cancer Research & Oncology Virtual Conference is now On-Demand! LabRoots invites research professionals, scientists, and clinicians to this premier online co...
APR 10, 2017
6:00 AM PDT
APR 10, 2017
6:00 AM PDT
The Protein and cell analysis education site is a free-access destination where you can learn more about applications and techniques related to protein gel chemistries, western detection, mas...
JAN 25, 2017
5:00 AM PST
JAN 25, 2017
5:00 AM PST
The Abcam virtual exhibition is temporarily closed. It will re-open shortly. Please check back October 25th. All webinars will be available for viewing at that time.Get the su...
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