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        High-throughput CRISPR editing using the Onyx™ platform identifies essential residues in proteins
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        The inhibitory role of specific macrophages in RCC
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        A novel automated and simultaneous protein/antibody high-throughput purification platform: AmMag and its reusable Magbeads
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        Uterine B cell dynamics during pregnancy - Immune regulation of the placenta
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        Utilization of Optical Genome Mapping in Detection and Characterization of Rare Genetic Markers in Pediatric Leukemias
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        An introduction to immuno-oncology at Miltenyi Biotec
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        Endpoint assays for the characterization of rare and therapy-resistant cells in cancer
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    Western Blot

    The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used in molecular biology, immunogenetics and other molecular biology disciplines to detect specific proteins in a sample of tissue homogenate or extract. In brief, the sample undergoes protein denaturation, followed by gel electrophoresis. Synthetic or animal-derived antibodies are created that recognise and bind to a specific target protein, known as the primary antibody. The electrophoresis membrane is washed in a solution containing the primary antibody, before excess antibody is washed off. A secondary antibody is added which recognises and binds to the primary antibody. The secondary antibody is visualised through various methods such as staining, immunofluorescence, and radioactivity, allowing indirect detection of the specific target protein.

    What's Popular in Western Blot

    Trending News

    • JAN 27, 2019
      Neuroscience
      Possible Biomarker Discovered for Schizophrenia
      JAN 27, 2019
      Possible Biomarker Discovered for Schizophrenia
      A study out of John's Hopkins University published by Dr. Faith Dickerson, MD, and colleagues late last year have fo ...
      WRITTEN BY: Amy Loriaux
      OCT 29, 2018
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      Gut: a second brain and novel therapeutic target
      OCT 29, 2018
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      Undersatnding the role of gut microbiome in disease pathologies and targetting them for potential treatment strategies.
      WRITTEN BY: Aswini Kanneganti
    JAN 27, 2019
    Neuroscience
    Possible Biomarker Discovered for Schizophrenia
    JAN 27, 2019
    Possible Biomarker Discovered for Schizophrenia
    A study out of John's Hopkins University published by Dr. Faith Dickerson, MD, and colleagues late last year have fo ...
    WRITTEN BY: Amy Loriaux
    OCT 29, 2018
    Neuroscience
    Gut: a second brain and novel therapeutic target
    OCT 29, 2018
    Gut: a second brain and novel therapeutic target
    Undersatnding the role of gut microbiome in disease pathologies and targetting them for potential treatment strategies.
    WRITTEN BY: Aswini Kanneganti

    Webinars

    • FEB 24, 2021 10:00 AM PST
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      C.E. CREDITS
      Biosafety Enclosures and the Automated Lab: Steps for Success
      FEB 24, 2021 10:00 AM PST
      Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
      Biosafety Enclosures and the Automated Lab: Steps for Success
      DATE: February 24, 2021 TIME: 10am PST Automated lab instruments such as liquid handlers and cell sorters are increasingly common in all types of laboratories, driving fast results for labor...
      Speaker: David Wasescha , Simon Fogarty
      Sponsored By: Labconco
      OCT 08, 2020 7:00 AM PDT
      C.E. CREDITS
      Protect your cells with proper pipetting - How liquid handling influences your cell culture work
      OCT 08, 2020 7:00 AM PDT
      Protect your cells with proper pipetting - How liquid handling...
      DATE: October 8, 2020 TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop thinking about ho...
      Speaker: Nadine Mellies, PhD , Jessica Wagener, PhD
      Sponsored By: Eppendorf
      SEP 22, 2020 11:00 AM PDT
      C.E. CREDITS
      A Novel Method for Quantitative  Western Blotting that Eliminates Errors from the Use of  Housekeeping Proteins
      SEP 22, 2020 11:00 AM PDT
      A Novel Method for Quantitative Western Blotting that Eliminates...
      DATE: August 22, 2020 TIME: 9:00am PDT The western blotting technique that began as a means of detecting the protein of interest in a complex sample that may contain numerous other proteins...
      Speaker: Alok Tomar, PhD
      Sponsored By: Thermo Fisher Scientific
      SEP 03, 2020 9:00 AM PDT
      C.E. CREDITS
      The Complexities of SV Analytical Ultracentrifugation: AAVs are not Simple, Binary Systems
      SEP 03, 2020 9:00 AM PDT
      The Complexities of SV Analytical Ultracentrifugation: AAVs are...
      DATE: September 3, 2020 TIME: 09:00am PT, 12:00pm ET xxx Learning Objectives: xxx Webinars will be available for unlimited on-demand viewing after live event. LabRoots is approved as a provi...
      Speaker: Dr. Lake Paul
      Sponsored By: Beckman Coulter Life Sciences
      JUL 08, 2020 8:00 AM PDT
      C.E. CREDITS
      Advancing Targeted Protein Degradation Research with Automated Western Blotting
      JUL 08, 2020 8:00 AM PDT
      Advancing Targeted Protein Degradation Research with Automated...
      DATE: July 8, 2020 TIME: 8:00am PDT In this webinar, discover how targeted protein degradation is being used to drive drug discovery. Hosted by Gary Allenby, Chief Executive Officer of Aurel...
      Speaker: Dr. Gary Allenby
      Sponsored By: Bio-Techne
    FEB 24, 2021 10:00 AM PST
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    Biosafety Enclosures and the Automated Lab: Steps for Success
    FEB 24, 2021 10:00 AM PST
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    Biosafety Enclosures and the Automated Lab: Steps for Success
    DATE: February 24, 2021 TIME: 10am PST Automated lab instruments such as liquid handlers and cell sorters are increasingly common in all types of laboratories, driving fast results for labor...
    Speaker: David Wasescha , Simon Fogarty
    Sponsored By: Labconco
    OCT 08, 2020 7:00 AM PDT
    C.E. CREDITS
    Protect your cells with proper pipetting - How liquid handling influences your cell culture work
    OCT 08, 2020 7:00 AM PDT
    Protect your cells with proper pipetting - How liquid handling...
    DATE: October 8, 2020 TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop thinking about ho...
    Speaker: Nadine Mellies, PhD , Jessica Wagener, PhD
    Sponsored By: Eppendorf
     
     
    SEP 22, 2020 11:00 AM PDT
    C.E. CREDITS
    A Novel Method for Quantitative  Western Blotting that Eliminates Errors from the Use of  Housekeeping Proteins
    SEP 22, 2020 11:00 AM PDT
    A Novel Method for Quantitative Western Blotting that Eliminates...
    DATE: August 22, 2020 TIME: 9:00am PDT The western blotting technique that began as a means of detecting the protein of interest in a complex sample that may contain numerous other proteins...
    Speaker: Alok Tomar, PhD
    Sponsored By: Thermo Fisher Scientific
    SEP 03, 2020 9:00 AM PDT
    C.E. CREDITS
    The Complexities of SV Analytical Ultracentrifugation: AAVs are not Simple, Binary Systems
    SEP 03, 2020 9:00 AM PDT
    The Complexities of SV Analytical Ultracentrifugation: AAVs are...
    DATE: September 3, 2020 TIME: 09:00am PT, 12:00pm ET xxx Learning Objectives: xxx Webinars will be available for unlimited on-demand viewing after live event. LabRoots is approved as a provi...
    Speaker: Dr. Lake Paul
    Sponsored By: Beckman Coulter Life Sciences
    JUL 08, 2020 8:00 AM PDT
    C.E. CREDITS
    Advancing Targeted Protein Degradation Research with Automated Western Blotting
    JUL 08, 2020 8:00 AM PDT
    Advancing Targeted Protein Degradation Research with Automated...
    DATE: July 8, 2020 TIME: 8:00am PDT In this webinar, discover how targeted protein degradation is being used to drive drug discovery. Hosted by Gary Allenby, Chief Executive Officer of Aurel...
    Speaker: Dr. Gary Allenby
    Sponsored By: Bio-Techne
    JUN 18, 2020 9:00 AM PDT
    C.E. CREDITS
    Unveiling the role of T cell dysfunction in the development and progression of glioblastoma
    JUN 18, 2020 9:00 AM PDT
    Unveiling the role of T cell dysfunction in the development and...
    DATE: June 18, 2020 TIME: 9:00am PDT, 12:00pm EDT Despite the success of immunotherapy against several malignancies including melanoma and lung adenocarcinoma, glioblastoma remains the excep...
    Speaker: Felipe Galvez Cancino, Ph.D.
    Sponsored By: Thermo Fisher Scientific/Gibco
    JUN 03, 2020 2:00 PM PDT
    C.E. CREDITS
    Dynamic Live Cell Imaging of Adherent and Immune Cells using CellASIC® ONIX2 Microfluidic Platform
    JUN 03, 2020 2:00 PM PDT
    Dynamic Live Cell Imaging of Adherent and Immune Cells using CellASIC®...
    While providing indispensable insight into immune processes, the live cell imaging of immune cells poses unique challenges due to their non-adherent nature. Suspension cells such as immune c...
    Speaker: Jun Park, PhD
    Presented at: Immuno-Oncology 2020Sponsored By: MilliporeSigma
    APR 28, 2020 10:00 AM PDT
    C.E. CREDITS
    Measuring Single EVs and their Cargo: Sensitive and Specific Vesicle Flow Cytometry (vFCTM)
    APR 28, 2020 10:00 AM PDT
    Measuring Single EVs and their Cargo: Sensitive and Specific Vesicle...
    DATE: April 28, 2020 TIME: 10:00am PT All cells release extracellular vesicles (EVs) that can carry molecular cargo to other cells to affect their function. EVs are promising as potential bi...
    Speaker: John Nolan, PhD
    Sponsored By: Beckman Coulter Life Sciences
    APR 14, 2020 9:00 AM PDT
    Gibco Cell Culture Heroes: Work From Home Edition
    APR 14, 2020 9:00 AM PDT
    Gibco Cell Culture Heroes: Work From Home Edition
    DATE: April 14, 2020 TIME: 9:00am PDT, 12:00pm EDT Besides being Gibco Cell Culture Heroes, what do Vivek, Sandra, Daisy, Ameet and Kristine all have in common? They are all stuck at home fo...
    Speaker: Kristine Wadosky, PhD , Ameet Chimote, PhD , Elizabeth Delery, PhD , Daisy Shu, B.Optom, PhD , Vivek Kamat, PhD , Sandra Hammer, PhD
    Sponsored By: Thermo Fisher Scientific/Gibco
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    Western Blot
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    Virtual Events

    • APR 29, 2020 6:00 AM PDT
      xMAP® Connect Virtual Conference
      APR 29, 2020 6:00 AM PDT
      xMAP® Connect Virtual Conference
      Luminex's xMAP® Technology enables the evaluation of up to 500 analytes simultaneously in a single well, known as multiplexing. xMAP Technology uses color-coded microspheres as the substrate...
      OCT 10, 2018 6:00 AM PDT
      Cancer Research & Oncology 2018
      OCT 10, 2018 6:00 AM PDT
      Cancer Research & Oncology 2018
      The 6th Annual Cancer Research & Oncology Virtual Event will take place October 2018. The free, two-day online event will bring research professionals, scientists, and clinicians from aro...
      OCT 03, 2018 6:00 AM PDT
      CRISPR 2018
      OCT 03, 2018 6:00 AM PDT
      CRISPR 2018
      LabRoots invites you to the First Annual CRISPR Virtual Event! With continued adoption and integration in research labs, CRISPR gene editing has gained importance both as a research tool in d...
      SEP 17, 2018
      3rd Annual 7 Steps of Protein Virtual Event
      SEP 17, 2018
      3rd Annual 7 Steps of Protein Virtual Event
      Improve your protein research knowledge by joining our 7 Steps of Protein virtual eventThe 3rd annual 7 Steps of Protein virtual event is taking place around the world on Tuesday, Septemb...
      Gibco ExpressionWorld 2018
      Gibco ExpressionWorld 2018
      Experience the latest advances in protein expression at our 3rd annual virtual event In 2017, the virtual event had 2,400 international participants in over 80 countries! Th...
    APR 29, 2020 6:00 AM PDT
    xMAP® Connect Virtual Conference
    APR 29, 2020 6:00 AM PDT
    xMAP® Connect Virtual Conference
    Luminex's xMAP® Technology enables the evaluation of up to 500 analytes simultaneously in a single well, known as multiplexing. xMAP Technology uses color-coded microspheres as the substrate...
     
     
    OCT 10, 2018 6:00 AM PDT
    Cancer Research & Oncology 2018
    OCT 10, 2018 6:00 AM PDT
    Cancer Research & Oncology 2018
    The 6th Annual Cancer Research & Oncology Virtual Event will take place October 2018. The free, two-day online event will bring research professionals, scientists, and clinicians from aro...
    OCT 03, 2018 6:00 AM PDT
    CRISPR 2018
    OCT 03, 2018 6:00 AM PDT
    CRISPR 2018
    LabRoots invites you to the First Annual CRISPR Virtual Event! With continued adoption and integration in research labs, CRISPR gene editing has gained importance both as a research tool in d...
    SEP 17, 2018
    3rd Annual 7 Steps of Protein Virtual Event
    SEP 17, 2018
    3rd Annual 7 Steps of Protein Virtual Event
    Improve your protein research knowledge by joining our 7 Steps of Protein virtual eventThe 3rd annual 7 Steps of Protein virtual event is taking place around the world on Tuesday, Septemb...
    Gibco ExpressionWorld 2018
    Gibco ExpressionWorld 2018
    Experience the latest advances in protein expression at our 3rd annual virtual event In 2017, the virtual event had 2,400 international participants in over 80 countries! Th...
    OCT 11, 2017 6:00 AM PDT
    Cancer Research & Oncology 2017
    OCT 11, 2017 6:00 AM PDT
    Cancer Research & Oncology 2017
    The 5th Annual LabRoots Cancer Research & Oncology Virtual Conference is now On-Demand!  LabRoots invites research professionals, scientists, and clinicians to this premier online co...
    APR 10, 2017 6:00 AM PDT
    Protein and Cell Analysis Education Series
    APR 10, 2017 6:00 AM PDT
    Protein and Cell Analysis Education Series
    The Protein and cell analysis education site is a free-access destination where you can learn more about applications and techniques related to protein gel chemistries, western detection, mas...
    JAN 25, 2017 5:00 AM PST
    Abcam virtual exhibition: support and products to advance your research
    JAN 25, 2017 5:00 AM PST
    Abcam virtual exhibition: support and products to advance your...
    The Abcam virtual exhibition is temporarily closed.  It will re-open shortly.  Please check back October 25th. All webinars will be available for viewing at that time.Get the su...
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    Western Blot
    Virtual Events
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