Western Blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used in molecular biology, immunogenetics and other molecular biology disciplines to detect specific proteins in a sample of tissue homogenate or extract. In brief, the sample undergoes protein denaturation, followed by gel electrophoresis. Synthetic or animal-derived antibodies are created that recognise and bind to a specific target protein, known as the primary antibody. The electrophoresis membrane is washed in a solution containing the primary antibody, before excess antibody is washed off. A secondary antibody is added which recognises and binds to the primary antibody. The secondary antibody is visualised through various methods such as staining, immunofluorescence, and radioactivity, allowing indirect detection of the specific target protein.
What's Popular in Western Blot
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JAN 27, 2019Neuroscience
JAN 27, 2019A study out of John's Hopkins University published by Dr. Faith Dickerson, MD, and colleagues late last year have fo ...WRITTEN BY: Amy LoriauxOCT 29, 2018Neuroscience
OCT 29, 2018Undersatnding the role of gut microbiome in disease pathologies and targetting them for potential treatment strategies.WRITTEN BY: Aswini Kanneganti
JAN 27, 2019
Neuroscience
JAN 27, 2019
A study out of John's Hopkins University published by Dr. Faith Dickerson, MD, and colleagues late last year have fo
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WRITTEN BY:
Amy Loriaux
OCT 29, 2018
Neuroscience
OCT 29, 2018
Undersatnding the role of gut microbiome in disease pathologies and targetting them for potential treatment strategies.
WRITTEN BY:
Aswini Kanneganti
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OCT 08, 2020 7:00 AM PDTC.E. CREDITS
OCT 08, 2020 7:00 AM PDTDATE: October 8, 2020 TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop thinking about ho...Speaker: Nadine Mellies, PhD , Jessica Wagener, PhDSponsored By: EppendorfSEP 22, 2020 11:00 AM PDTC.E. CREDITS
SEP 22, 2020 11:00 AM PDTDATE: August 22, 2020 TIME: 9:00am PDT The western blotting technique that began as a means of detecting the protein of interest in a complex sample that may contain numerous other proteins...Speaker: Alok Tomar, PhDSponsored By: Thermo Fisher ScientificSEP 03, 2020 9:00 AM PDTC.E. CREDITS
SEP 03, 2020 9:00 AM PDTDATE: September 3, 2020 TIME: 09:00am PT, 12:00pm ET xxx Learning Objectives: xxx Webinars will be available for unlimited on-demand viewing after live event. LabRoots is approved as a provi...Speaker: Dr. Lake PaulSponsored By: Beckman Coulter Life SciencesJUL 08, 2020 8:00 AM PDTC.E. CREDITS
JUL 08, 2020 8:00 AM PDTDATE: July 8, 2020 TIME: 8:00am PDT In this webinar, discover how targeted protein degradation is being used to drive drug discovery. Hosted by Gary Allenby, Chief Executive Officer of Aurel...Speaker: Dr. Gary AllenbySponsored By: Bio-TechneJUN 18, 2020 9:00 AM PDTC.E. CREDITS
JUN 18, 2020 9:00 AM PDTDATE: June 18, 2020 TIME: 9:00am PDT, 12:00pm EDT Despite the success of immunotherapy against several malignancies including melanoma and lung adenocarcinoma, glioblastoma remains the excep...Speaker: Felipe Galvez Cancino, Ph.D.Sponsored By: Thermo Fisher Scientific/Gibco
OCT 08, 2020
7:00 AM PDT
C.E. CREDITS
OCT 08, 2020
7:00 AM PDT
DATE: October 8, 2020 TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop thinking about ho...
Speaker:
Nadine Mellies, PhD
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Jessica Wagener, PhD
Sponsored By: Eppendorf
SEP 22, 2020
11:00 AM PDT
C.E. CREDITS
SEP 22, 2020
11:00 AM PDT
DATE: August 22, 2020 TIME: 9:00am PDT The western blotting technique that began as a means of detecting the protein of interest in a complex sample that may contain numerous other proteins...
Speaker:
Alok Tomar, PhD
Sponsored By: Thermo Fisher Scientific
SEP 03, 2020
9:00 AM PDT
C.E. CREDITS
SEP 03, 2020
9:00 AM PDT
DATE: September 3, 2020 TIME: 09:00am PT, 12:00pm ET xxx Learning Objectives: xxx Webinars will be available for unlimited on-demand viewing after live event. LabRoots is approved as a provi...
Speaker:
Dr. Lake Paul
Sponsored By: Beckman Coulter Life Sciences
JUL 08, 2020
8:00 AM PDT
C.E. CREDITS
JUL 08, 2020
8:00 AM PDT
DATE: July 8, 2020 TIME: 8:00am PDT In this webinar, discover how targeted protein degradation is being used to drive drug discovery. Hosted by Gary Allenby, Chief Executive Officer of Aurel...
Speaker:
Dr. Gary Allenby
Sponsored By: Bio-Techne
JUN 18, 2020
9:00 AM PDT
C.E. CREDITS
JUN 18, 2020
9:00 AM PDT
DATE: June 18, 2020 TIME: 9:00am PDT, 12:00pm EDT Despite the success of immunotherapy against several malignancies including melanoma and lung adenocarcinoma, glioblastoma remains the excep...
Speaker:
Felipe Galvez Cancino, Ph.D.
Sponsored By: Thermo Fisher Scientific/Gibco
APR 28, 2020
10:00 AM PDT
C.E. CREDITS
APR 28, 2020
10:00 AM PDT
DATE: April 28, 2020 TIME: 10:00am PT All cells release extracellular vesicles (EVs) that can carry molecular cargo to other cells to affect their function. EVs are promising as potential bi...
Speaker:
John Nolan, PhD
Sponsored By: Beckman Coulter Life Sciences
APR 14, 2020
9:00 AM PDT
APR 14, 2020
9:00 AM PDT
DATE: April 14, 2020 TIME: 9:00am PDT, 12:00pm EDT Besides being Gibco Cell Culture Heroes, what do Vivek, Sandra, Daisy, Ameet and Kristine all have in common? They are all stuck at home fo...
Speaker:
Kristine Wadosky, PhD
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Ameet Chimote, PhD
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Elizabeth Delery, PhD
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Daisy Shu, B.Optom, PhD
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Vivek Kamat, PhD
,
Sandra Hammer, PhD
Sponsored By: Thermo Fisher Scientific/Gibco
MAR 27, 2020
9:00 AM PDT
C.E. CREDITS
MAR 27, 2020
9:00 AM PDT
DATE: March 27, 2020 TIME: 9:00am PDT, 12:00pm EDT Osteoporosis causes a decrease in bone density, along with deterioration of the bone’s microarchitecture at a faster rate than normal...
Speaker:
Mayda Arshad
Sponsored By: Thermo Fisher Scientific/Gibco
JAN 30, 2020
9:00 AM PST
C.E. CREDITS
JAN 30, 2020
9:00 AM PST
DATE: January 30, 2020 TIME: 9:00am PST, 12:00pm EST Recent advances in in vitro 3D cellular culture technologies, such as organoids, rapidly developed and established novel, more physiologi...
Speaker:
Brooke Schuster
Sponsored By: Thermo Fisher Scientific/Gibco
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APR 29, 2020 6:00 AM PDT
APR 29, 2020 6:00 AM PDTLuminex's xMAP® Technology enables the evaluation of up to 500 analytes simultaneously in a single well, known as multiplexing. xMAP Technology uses color-coded microspheres as the substrate...OCT 10, 2018 6:00 AM PDT
OCT 10, 2018 6:00 AM PDTThe 6th Annual Cancer Research & Oncology Virtual Event will take place October 2018. The free, two-day online event will bring research professionals, scientists, and clinicians from aro...OCT 03, 2018 6:00 AM PDT
OCT 03, 2018 6:00 AM PDTLabRoots invites you to the First Annual CRISPR Virtual Event! With continued adoption and integration in research labs, CRISPR gene editing has gained importance both as a research tool in d...SEP 17, 2018
SEP 17, 2018Improve your protein research knowledge by joining our 7 Steps of Protein virtual eventThe 3rd annual 7 Steps of Protein virtual event is taking place around the world on Tuesday, Septemb...
Experience the latest advances in protein expression at our 3rd annual virtual event In 2017, the virtual event had 2,400 international participants in over 80 countries! Th...
APR 29, 2020
6:00 AM PDT
APR 29, 2020
6:00 AM PDT
Luminex's xMAP® Technology enables the evaluation of up to 500 analytes simultaneously in a single well, known as multiplexing. xMAP Technology uses color-coded microspheres as the substrate...
OCT 10, 2018
6:00 AM PDT
OCT 10, 2018
6:00 AM PDT
The 6th Annual Cancer Research & Oncology Virtual Event will take place October 2018. The free, two-day online event will bring research professionals, scientists, and clinicians from aro...
OCT 03, 2018
6:00 AM PDT
OCT 03, 2018
6:00 AM PDT
LabRoots invites you to the First Annual CRISPR Virtual Event! With continued adoption and integration in research labs, CRISPR gene editing has gained importance both as a research tool in d...
SEP 17, 2018
SEP 17, 2018
Improve your protein research knowledge by joining our 7 Steps of Protein virtual eventThe 3rd annual 7 Steps of Protein virtual event is taking place around the world on Tuesday, Septemb...
Experience the latest advances in protein expression at our 3rd annual virtual event In 2017, the virtual event had 2,400 international participants in over 80 countries! Th...
OCT 11, 2017
6:00 AM PDT
OCT 11, 2017
6:00 AM PDT
The 5th Annual LabRoots Cancer Research & Oncology Virtual Conference is now On-Demand! LabRoots invites research professionals, scientists, and clinicians to this premier online co...
APR 10, 2017
6:00 AM PDT
APR 10, 2017
6:00 AM PDT
The Protein and cell analysis education site is a free-access destination where you can learn more about applications and techniques related to protein gel chemistries, western detection, mas...
JAN 25, 2017
5:00 AM PST
JAN 25, 2017
5:00 AM PST
The Abcam virtual exhibition is temporarily closed. It will re-open shortly. Please check back October 25th. All webinars will be available for viewing at that time.Get the su...
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