FEB 09, 2021 9:00 AM PST

Development and analytical validation of prognostic biomarkers for metastasis

Sponsored by: Visiopharm®
C.E. Credits: P.A.C.E. CE Florida CE
Speaker
  • Assistant Professor, Department of Anatomy and Structural Biology, Albert Einstein College of Medicine
    Biography
      Dr. David Entenberg is an Assistant Professor in the Department of Anatomy and Structural Biology at Albert Einstein College of Medicine. He received his bachelor’s degree in physics at SUNY Stony Brook. After receiving a Masters’ degree in chemical physics from Weizmann Institute of Science in Israel, Dr. Entenberg worked at Tellabs as an optical test engineer where he supported a manufacturing production line for telecommunications equipment, and then as a research engineer at Memorial Sloan-Kettering Cancer Center where he transitioned into the biological sciences by developing novel instrumentation and engineering based assays for biological research. He obtained his Ph.D. in cell biology from the University of Kent where the title of his thesis was “Enabling research into the tumor microenvironment: Novel Photonic Assays for Cancer Research”. He brought his imaging expertise to Albert Einstein College of Medicine, where he’s been since 2006 and now serves as the Director of Technology Development for Einstein’s Gruss Lipper Biophotonics Center and its associated Integrated Imaging Program. Dr. Entenberg leads the development of novel and innovative imaging techniques that provide high-resolution visualization of the tumor microenvironment. He has collaborated closely with Dr. John Condeelis in the development of intravital imaging windows in which they were able to visualize the different stages of cancer progression and the interactions between tumor cells and macrophages in the tumor microenvironment, and with Drs. Maja Oktay and Joseph Sparano on the development and validation of clinical biomarkers for metastasis. Dr. Entenberg’s work has been published in such high impact journals as Nature Methods and Nature Cell Biology. In addition, his work has made the cover of Nature Cell Biology, Intravital, and Science Translational Medicine.

    Abstract
    Date:  February 9, 2021
    Time: 9:00am (PST),  12:00pm (EST),  6:00pm (CET)
     
    90% of breast cancer mortality is caused by distant metastasis, a process that involves both dissemination of cancer cells to distant sites as well as their proliferation after arrival. However, prognostic assays currently used in the clinic are based on proliferation and do not measure tumor cell dissemination potential. Based on intravital imaging of tumor cell dissemination in live animals, we previously reported two biomarkers for the development of distant metastasis: TMEM Score and MenaCalc. TMEM Score is based upon the density of Tumor Microenvironment of Metastasis (TMEM) doorways, portals for cancer cell intravasation and dissemination formed by the confluence of a Mena overexpressing tumor cell, a pro-angiogenic macrophage, and an endothelial cell. MenaCalc is a pattern of expression of the actin-regulatory protein Mena which leads tumor cells to undergo epithelial-to-mesenchymal transition (EMT) and become highly motile. Using digital pathology, we analytically validated an automated analysis of TMEM doorways that reduced pathologist time by an order of magnitude and enabled the rapid clinical validation of TMEM Score. While TMEM score has been validated for prognosticating metastatic outcome in HR+/HER2- patients, statistical significance was not observed in patients with triple negative or HER2+ breast cancers. Furthermore, MenaCalc has been shown to be prognostic in some cohorts of patients with triple negative disease but the prognostic value of MenaCalc in HR+ disease is still unclear. Since TMEM doorways and MenaCalc are mechanistically linked (but independent) biomarkers, we investigated if a combined TMEM-MenaCalc biomarker can improve the prognostication ability of either biomarker alone. Again, using digital pathology, we evaluated several different methods of combining TMEM and MenaCalc scores to create a multiparameter quantitative analysis with dramatically improved prognostic ability for distant metastasis in breast cancer patients.
     
    Learning Objectives
    • Discuss the process of tumor cell dissemination from breast tumors.
    • Summarize how live imaging studies are able to discover mechanisms of metastasis which can then be turned into prognostic biomarkers for metastasis.
    • Outline how digital whole slide scanning and digital pathology can be used to automate as well as analytically and clinically validate prognostic biomarkers.
     
     
     
    Webinars will be available for unlimited on-demand viewing after live event.
     
    LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.

    Show Resources
    You May Also Like
    MAY 11, 2021 10:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    MAY 11, 2021 10:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    Date: May 11, 2021 Time: 10:00zm PDT Your samples are some of the most valuable assets in the laboratory. After spending countless hours on extraction and preparation, your conclusions could...
    NOV 18, 2020 8:00 AM PST
    C.E. CREDITS
    NOV 18, 2020 8:00 AM PST
    DATE: November 18, 2020 TIME: 08:00am PDT We develop and implement technologies to solve some of the major bottlenecks in biomedical research. In particular, we establish new imaging approac...
    APR 01, 2021 8:00 AM PDT
    C.E. CREDITS
    APR 01, 2021 8:00 AM PDT
    Date: April 01, 2021 Time: 8:00am (PST), 11:00am (EST) Generating therapeutic antibodies is far more challenging than obtaining antibodies that merely recognize their targets. Engineering po...
    OCT 29, 2020 6:00 AM PDT
    C.E. CREDITS
    OCT 29, 2020 6:00 AM PDT
    Date: October 29, 2020 Time: 6:00am (PDT), 9:00am (EDT), Chronic inflammation can occur as a result of a combination of genetic predispositions and environmental factors. Epigenetic modifica...
    NOV 16, 2020 8:00 AM PST
    C.E. CREDITS
    NOV 16, 2020 8:00 AM PST
    Date: November 16, 2020 Time: 8:00am (PST), 11:00am (EST) CRISPR screening has become the prime discovery tool in modern biomedical research and drug discovery. At the same time, most screen...
    DEC 02, 2020 8:00 AM PST
    C.E. CREDITS
    DEC 02, 2020 8:00 AM PST
    DATE: December 2nd, 2020 TIME: 08:00am PDT, 11:00pm EDT Bioreactors and shakers are used to cultivate microorganisms, plant, insect, and mammalian cells in different volumes. Upscaling of pr...
    Loading Comments...
    Show Resources
    Attendees
    • See more