There is significant epidemiological evidence to suggest that the consumption of a high-broccoli diet is associated with a reduced risk of cancer and cardiovascular disease. Human intervention studies have shown that a broccoli-rich diet can reduce cholesterol levels and rebalance central cell metabolism. Our studies also show that the effect of diet is also influenced by the allelic status of a gene known as PAPOLG, an RNA poly(A) polymerase not previously associated metabolic homeostasis. Through the use of in vitro cell models, high throughput metabolomics and live cell energy phenotyping, the nutrigenetic relationship between PAPOLG and the broccoli-rich diet can be explored. In this webinar I will expand upon the link between broccoli bioactives and metabolic homeostasis, highlight the potential role of RNA turnover in metabolic control, and offer insights into the potential implications for the dietary prevention of disease.