MAY 01, 2017 08:00 AM PDT
Improved differentiation of stem cell-derived neurons and downstream applications through the reduction of progenitor proliferation with CultureOne™ Supplement
Presented at the Gibco 24 Hours of Stem Cells virtual event Virtual Event
1 2 71

Speakers:
  • Associate Director and Group Leader, Thermo Fisher Scientific
    Biography
      Dr. Kuninger leads research, development and commercialization of next generation culture systems for pluripotent stem cell culture, differentiation, neurobiology and non-hepatic primary cell biology at Thermo Fisher Scientific in the Cell Biology business based in Frederick MD. His teams support numerous portfolios and have launched over 15 new products spanning stem cell culture & cryopreservation, differentiation (endo-, ecto- and meso-dermal lineages) and neurobiology, leading to more than 25M in revenue over the last 3 years. David is a seasoned scientist and manager, experienced in media formulation & optimization as well as with sophisticated experimental design and data modeling tools applied to cell imaging and functional analysis endpoints as well as 2D and 3D culture models. Expertise in GLP/GMP compliance, technical transfer and scale up, verification and validation processes. Prior to starting at Thermo Fisher Scientific (legacy Invitrogen) in 2007 as Staff Scientist, he joined Oregon Health Sciences University (OHSU) as a Postdoctoral Fellow investigating the actions of insulin-like growth factors (IGFs) in the lab of Dr. Peter Rotwein, subsequently joining the faculty in the Department of Biochemistry at OHSU as a Research Instructor. He completed is PhD in Biochemistry and Genetics University of Texas Medical Branch, Galveston, Texas in the laboratory of Dr. John Papaconstatinou and has a B.S. in Chemistry from the University of Oregon in Eugene OR.

    Abstract:

    Neurons derived from human pluripotent stem cells (hPSCs) and primary rodent neurons both are excellent resources for disease modeling and drug screening.  Human PSCs derived neural stem cells (NSCs) can be expanded in culture and further differentiated into mature neurons for various applications, however, these often contain mixed population of both differentiated neurons and undifferentiated NSCs. Due to the continuing proliferation of undifferentiated NSCs, very high cell densities and cell aggregation are usually observed during the differentiation of hPSC-derived NSCs which increase over time, posing challenges for long-term maintenance and downstream analysis.  Primary rodent neuronal cultures, while highly physiologically relevant, are often challenged by glial cell overgrowth, which may exacerbate assay and analysis issues.  Here we demonstrate the use of CultureOne(tm) - a new supplement which can reduce the proliferation of undifferentiated NSCs without negatively impacting the rate or extent of differentiation for hPSC-derived NSCs.  Further, we will demonstrate the ability to "tune" the glial cell population in primary rodent neuronal cultures.  The overall effect in both instances increases the relevant population of neurons in culture.  Experimental data presented in this webinar will  illustrate the functionality, morphology, and maturity of these neuronal cultures.


    Show Resources
    Loading Comments...