SEP 10, 2020 2:00 PM PDT

Dynamic live yeast and bacterial cell imaging using CellASIC ONIX2 microfluidic platform

Sponsored by: MilliporeSigma
C.E. Credits: P.A.C.E. CE Florida CE
Speaker
  • Applications Lead
    Biography

      Cindy Chen originally comes from Taiwan. After completing her Bachelor Degree in Clinical Laboratory at Taipei Medical University, she continued her studies in Biochemistry and Molecular Biology at University of California, Davis, where her Ph.D. thesis was focused on thyroid hormone-regulated gene expression cascades during Xenopus laevis metamorphosis. She joined CellASIC as a scientist in 2009 which was later acquired by EMD Millipore in 2014. She has continued working with the microfluidic R&D team to create a portfolio of the microfluidic cell culture plates that can accommodate researchers from microbial to mammalian studies to monitor cellular responses to environmental perturbations under the microscopes for both short and long terms.


    Abstract

    While live cell imaging offers advantages over traditional static imaging, this approach has been challenging for studying microbes due to the difficulty in tracking very small cells in a single focal plane during long term culture. Together with the CellASIC® ONIX2 Microfluidic System for live cell imaging, the CellASIC® Trap Plates offer unique architecture that positions nonadherent live cells in a focal plane to enable continuous imaging and simpler generational analysis of progeny over time.  In addition, these plates offer special features for monitoring biofilm growth and its response to environmental perturbation. Here, we report additional capability enabled by our next generation trap plate with a proprietary design that alleviates drawbacks previously imposed by cellular overgrowth. When combined with the ONIX2 system, the new plate offers an unprecedented turnkey solution to the mechanistic investigation of the impact of environmental conditions on microbes.

    Learning Objectives:

     


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