SEP 10, 2020 2:00 PM PDT

Dynamic live yeast and bacterial cell imaging using CellASIC ONIX2 microfluidic platform

Sponsored by: MilliporeSigma
C.E. Credits: P.A.C.E. CE Florida CE
Speaker
  • Applications Lead
    Biography

      Cindy Chen originally comes from Taiwan. After completing her Bachelor Degree in Clinical Laboratory at Taipei Medical University, she continued her studies in Biochemistry and Molecular Biology at University of California, Davis, where her Ph.D. thesis was focused on thyroid hormone-regulated gene expression cascades during Xenopus laevis metamorphosis. She joined CellASIC as a scientist in 2009 which was later acquired by EMD Millipore in 2014. She has continued working with the microfluidic R&D team to create a portfolio of the microfluidic cell culture plates that can accommodate researchers from microbial to mammalian studies to monitor cellular responses to environmental perturbations under the microscopes for both short and long terms.


    Abstract

    While live cell imaging offers advantages over traditional static imaging, this approach has been challenging for studying microbes due to the difficulty in tracking very small cells in a single focal plane during long term culture. Together with the CellASIC® ONIX2 Microfluidic System for live cell imaging, the CellASIC® Trap Plates offer unique architecture that positions nonadherent live cells in a focal plane to enable continuous imaging and simpler generational analysis of progeny over time.  In addition, these plates offer special features for monitoring biofilm growth and its response to environmental perturbation. Here, we report additional capability enabled by our next generation trap plate with a proprietary design that alleviates drawbacks previously imposed by cellular overgrowth. When combined with the ONIX2 system, the new plate offers an unprecedented turnkey solution to the mechanistic investigation of the impact of environmental conditions on microbes.

    Learning Objectives:

     


    Show Resources
    You May Also Like
    MAY 11, 2021 10:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    MAY 11, 2021 10:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    Date: May 11, 2021 Time: 10:00zm PDT Your samples are some of the most valuable assets in the laboratory. After spending countless hours on extraction and preparation, your conclusions could...
    JUN 09, 2021 7:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    JUN 09, 2021 7:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...
    DEC 02, 2020 8:00 AM PST
    C.E. CREDITS
    DEC 02, 2020 8:00 AM PST
    DATE: December 2nd, 2020 TIME: 08:00am PDT, 11:00pm EDT Bioreactors and shakers are used to cultivate microorganisms, plant, insect, and mammalian cells in different volumes. Upscaling of pr...
    MAR 16, 2021 10:00 AM PDT
    C.E. CREDITS
    MAR 16, 2021 10:00 AM PDT
    Date: March 16, 2021 Time: 10:00am (PST) Scientific progress and breakthroughs today are often too expensive for most institutions to acquire. Each year, the National Institutes of Health (N...
    NOV 16, 2020 8:00 AM PST
    C.E. CREDITS
    NOV 16, 2020 8:00 AM PST
    Date: November 16, 2020 Time: 8:00am (PST), 11:00am (EST) CRISPR screening has become the prime discovery tool in modern biomedical research and drug discovery. At the same time, most screen...
    NOV 18, 2020 8:00 AM PST
    C.E. CREDITS
    NOV 18, 2020 8:00 AM PST
    DATE: November 18, 2020 TIME: 08:00am PDT We develop and implement technologies to solve some of the major bottlenecks in biomedical research. In particular, we establish new imaging approac...
    Loading Comments...
    Show Resources