SEP 30, 2015 10:30 AM PDT

High efficiency SPRI (Solid Phase Reverse Immobilization Paramagnetic Bead) technology for microRNA applications

SPONSORED BY: Beckman Coulter Life Sciences
C.E. CREDITS: P.A.C.E. CE
Speakers
  • Staff Application Scientist, Beckman Coulter Life Sciences
    Biography
      Dr. Bee Na Lee has worked at Beckman Coulter Life Sciences for five years, where she is responsible for leading microRNA and RNA applications. She has 10 years of experience in genomic and genetics research. Prior to joining Beckman Coulter, she worked at the genomic institute at Syngenta, eGene (a start-up company), and Qiagen. She was the lead scientist in developing QIAxcel Genetic Analyzer and published more than 30 application methods, protocols and application notes. She has a PhD in Microbiology and Molecular Biology from Texas A&M University, and was a postdoctoral fellow at Harvard Medical School, sponsored by Leukemia Society of America. Her research experience was in the fields of fungal biology, cell signaling, small molecules and plan pathogenicity. She has published more than 15 peer reviewed scientific papers on cell signaling, development and pathogenicity

    Abstract:
    Micro RNAs (miRNAs) are small, naturally-occurring non-coding ribonucleic acids with sizes between 18 and 40 nucleotides (nts) that have been demonstrated to play a significant role in the regulation of gene expression.  As a result, interest in smaller RNA species such as miRNA has increased. This webinar describes the method development for miRNA purification, enrichment and RNA fractionation using Beckman Coulter’s SPRI (Solid Phase Reverse Immobilization) magnetic bead based chemistry and the Biomek automated extraction method.  Total RNA and miRNA can be purified from very small amounts of highly degraded FFPE (Formalin Fixed, Paraffin Embedded) and LCM (Laser Captured Microdissection) samples. The SPRI chemistry produced superior results to a column purification method for miRNA purification, miRNA enrichment and RNA size selection.  The SPRI chemistry is an easy, high-yielding and automation-friendly nucleic acid purification process that does not require centrifugation and vacuum filtration steps. Purified nucleic acids are easily eluted from the magnetic beads under aqueous conditions, which provide maximum flexibility for downstream applications.

    Learning Objectives:
    • Understand the method development for miRNA purification, enrichment and RNA fractionation using Beckman Coulter’s SPRI (Solid Phase Reverse Immobilization) magnetic bead based chemistry and the Biomek automated extraction method
    • Learn how total RNA and miRNA can be purified from very small amounts of highly degraded FFPE (Formalin Fixed, Paraffin Embedded) and LCM (Laser Captured Microdissection) samples

    Show Resources
    You May Also Like
    MAY 22, 2018 08:00 AM PDT
    C.E. CREDITS
    MAY 22, 2018 08:00 AM PDT
    DATE: May 22, 2018TIME: 08:00AM PDT The nuclear receptors pregnane X receptor (PXR) and constitutive androstane receptor (CAR) are closely related transcription factors that...
    MAY 03, 2018 11:00 AM PDT
    MAY 03, 2018 11:00 AM PDT
    DATE: May 3, 2018TIME: 11:00AM PDT, 2:00PM EDTWhile stress is one of the leading causes of neuropsychiatric disorders, the molecular underpinnings of how stress induces alterations in b...
    JUN 26, 2018 06:00 AM PDT
    C.E. CREDITS
    JUN 26, 2018 06:00 AM PDT
    Date: June 26, 2018Time: 6:00 a.m. PDT, 9:00 a.m. EDT, 1500 CEST Today’s hematology analyzers employ various methods for enumerating platelets. These methods include: e...
    MAY 24, 2018 09:30 AM PDT
    C.E. CREDITS
    MAY 24, 2018 09:30 AM PDT
    DATE: May 24, 2018 TIME: 9:30PM PDT The current gold standard in in vitro pre-clinical cancer treatment screening remain cell lines,...
    APR 27, 2018 10:00 AM PDT
    C.E. CREDITS
    APR 27, 2018 10:00 AM PDT
    DATE: April 27, 2018TIME: 10:00am PST, 1:00pm ESTGlioblastoma (GBM) and Medulloblastoma (MB) are the most common adult and paediatric brain tumours, both of which can have devastating c...
    JUN 20, 2018 10:00 AM EDT
    C.E. CREDITS
    JUN 20, 2018 10:00 AM EDT
    DATE: June 20, 2018TIME: 07:00AM PDT, 10:00AM EDTIntroducing GE’s New Lyo-StableTM service. Sepsis is one of the top challenges facing hospitals in terms of clinical outcomes...
    Loading Comments...