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SEP 30, 2015 10:30 AM PDT

High efficiency SPRI (Solid Phase Reverse Immobilization Paramagnetic Bead) technology for microRNA applications

Speaker
  • Staff Application Scientist, Beckman Coulter Life Sciences
    Biography
      Dr. Bee Na Lee has worked at Beckman Coulter Life Sciences for five years, where she is responsible for leading microRNA and RNA applications. She has 10 years of experience in genomic and genetics research. Prior to joining Beckman Coulter, she worked at the genomic institute at Syngenta, eGene (a start-up company), and Qiagen. She was the lead scientist in developing QIAxcel Genetic Analyzer and published more than 30 application methods, protocols and application notes. She has a PhD in Microbiology and Molecular Biology from Texas A&M University, and was a postdoctoral fellow at Harvard Medical School, sponsored by Leukemia Society of America. Her research experience was in the fields of fungal biology, cell signaling, small molecules and plan pathogenicity. She has published more than 15 peer reviewed scientific papers on cell signaling, development and pathogenicity

    Abstract
    Micro RNAs (miRNAs) are small, naturally-occurring non-coding ribonucleic acids with sizes between 18 and 40 nucleotides (nts) that have been demonstrated to play a significant role in the regulation of gene expression.  As a result, interest in smaller RNA species such as miRNA has increased. This webinar describes the method development for miRNA purification, enrichment and RNA fractionation using Beckman Coulter’s SPRI (Solid Phase Reverse Immobilization) magnetic bead based chemistry and the Biomek automated extraction method.  Total RNA and miRNA can be purified from very small amounts of highly degraded FFPE (Formalin Fixed, Paraffin Embedded) and LCM (Laser Captured Microdissection) samples. The SPRI chemistry produced superior results to a column purification method for miRNA purification, miRNA enrichment and RNA size selection.  The SPRI chemistry is an easy, high-yielding and automation-friendly nucleic acid purification process that does not require centrifugation and vacuum filtration steps. Purified nucleic acids are easily eluted from the magnetic beads under aqueous conditions, which provide maximum flexibility for downstream applications.

    Learning Objectives:
    • Understand the method development for miRNA purification, enrichment and RNA fractionation using Beckman Coulter’s SPRI (Solid Phase Reverse Immobilization) magnetic bead based chemistry and the Biomek automated extraction method
    • Learn how total RNA and miRNA can be purified from very small amounts of highly degraded FFPE (Formalin Fixed, Paraffin Embedded) and LCM (Laser Captured Microdissection) samples

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