MAY 10, 2017 7:30 AM PDT

Enrichment of cffDNA and ctDNA via size selection for reduced diagnostic assay costs

C.E. Credits: CEU
Speakers
  • President, Coastal Genomics
    Biography
      Mr. Nesbitt completed his Masters degree in Molecular Biology at Simon Fraser University where his work focused on the discovery of new genes in the C. elegans model organism. This experience impressed upon him both the value of next generation sequencing and its vulnerabilities to the extensively manual sample preparation steps. Mr. Nesbitt started his professional career in liquid handling automation as a product manager responsible for the development of NGS library construction solutions. He now works with Coastal Genomics to launch its automated gel size electrophoresis solution, Ranger Technology.
      <br />

    Abstract:

    Cell free DNA (cfDNA) analysis for the purpose of diagnostics is currently practiced for non-invasive prenatal screening of cell free fetal DNA (cffDNA), and is anticipated to be leveraged for early stage cancer detection via circulating tumour DNA (ctDNA) detection. The process can be expensive as cfDNA fragments originating from a source of interest (i.e. cffDNA, ctDNA) are greatly outnumbered by those from normal tissues. While different methodologies for enrichment are being devised to deal with this issue, recent evidence suggests that cffDNA and ctDNA may be largely degraded to a final size of a specific length range. Such a reality opens the door to enrichment via tight size selection. Coastal Genomics is working to validate its Ranger® Technology platform for automation of high resolution gel-based size selection. In this talk, we discuss progress on this front, and consider realistic implementation of size selection in the context of the tradeoff between assay specificity and sensitivity.


    Show Resources
    You May Also Like
    SEP 05, 2019 4:00 PM CEST
    C.E. CREDITS
    SEP 05, 2019 4:00 PM CEST
    DATE: September 5, 2019TIME: 7:00am PT, 10:00am ET, 4:00pm CEST PCR (Polymerase Chain Reaction) has gone through a massive evolution since its development in 1983. Besides it...
    JAN 23, 2020 9:00 AM PST
    C.E. CREDITS
    JAN 23, 2020 9:00 AM PST
    DATE: January 23, 2020 TIME: 9:00am PST, 12:00pm EST...
    NOV 18, 2019 7:00 AM PST
    C.E. CREDITS
    NOV 18, 2019 7:00 AM PST
    DATE: November 18, 2019TIME: 7:00am PST, 11:00am EST, 4:00pm CEWT How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop th...
    OCT 02, 2019 11:00 AM PDT
    OCT 02, 2019 11:00 AM PDT
    DATE: October 2, 2019TIME: 11:00am PDT, 2:00pm EDT Ditch the Excel spreadsheets and manage your molecular workflows entirely in your LIMS Achieve configuration of molecular workf...
    AUG 27, 2019 9:00 AM PDT
    C.E. CREDITS
    AUG 27, 2019 9:00 AM PDT
    DATE: August 27, 2019 TIME: 9:00am PDT, 12:00pm EDT Immunotherapies targeting PD-1 or PD-L1 have proven remarkably effective for treating cancer in some patients, with considerabl...
    FEB 25, 2020 9:00 AM PST
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    FEB 25, 2020 9:00 AM PST
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    Learn about how to generate a small scale CAR-T workflow using ThermoFisher products See detailed characterization tools that can be utilized and applied in a CAR-T workflow...
    Loading Comments...
    Show Resources