NOV 15, 2017 9:00 AM PST

WEBINAR: The essential role of miRNA and mRNA on cancer progression and biomarker discovery

Speaker
  • Head of Basic Research, Clinic for Gastroenterology, University of Marburg
    Biography
      Malte Buchholz obtained his PhD in molecular biology at the University of Goettingen, Germany, in 1998. He is group leader and Head of Basic Research at the Department of Gastroenterology, Endocrinology and Metabolism, University Hospital Marburg, Philipps-University of Marburg, Germany (http://www.uni-marburg.de/fb20/gastroendokrinol/forschung?language_sync=1). His research interests focus on transcriptome analyses and molecular diagnostics of gastrointestinal tumors, functional genomic analyses of pancreatic cancer, and molecular determinants of tumor-host interactions. Among others, he has performed global gene expression analyses of stromal cell populations from different organs as well as microdissected tumor cells and pre-neoplastic lesions from pancreatic ductal adenocarcinoma (PDAC) patients. These data formed an important basis for the design of specialized cDNA arrays which are suitable to distinguish between different forms of pancreatico-biliary tumors with high diagnostic precision. In addition, his group has identified and characterized novel candidate genes with central roles in the physiology of pancreatic tumor cells.
      Dr. Buchholz has been intimately involved in the management and coordination of several national and international multidisciplinary research projects, including the BMBF-funded NGFNplus project "PaCa-Net" as well as the EU FP7 collaborative projects "MolDiag-PaCa" and "European Pancreatic Cancer-Tumor-Microenvironment Network (EPC-TM-Net)". He is currently co-coordinator of the EU FP7 large-scale integrative project "CAM-PaC" (Integrative Analysis of Gene Functions in Cellular and Animal Models of Pancreatic Cancer)( http://www.cam-pac.eu/) and "Bio-PaC" (Biomarkers of tumor recurrence in pancreatic cancer), the latter of which focuses on the development of focused mixed TaqMan arrays as a diagnostic tool for the early detection of tumor recurrence in pancreatic cancer.

    Abstract

    DATE: November 15, 2017
    TIME: 09:00am PST, 12:00pm EST, 6:00pm CET

    MicroRNAs (miRNA) are a class of small non-coding RNAs (approximately 21 nt long) that bind complementary sequences in target mRNAs to specifically regulate gene expression. miRNAs play important roles in regulation of gene expression during cell development, differentiation, proliferation and apoptosis. Misregulation of miRNAs and their targets has been associated with several diseases including cancer. The interplay between miRNA and mRNA has been found to be important in cancer development and progression. Simultaneous expression studies of miRNA and mRNA can be valuable in understanding molecular mechanisms that may potentially have an underlying role in various diseases. In this webinar, we demonstrate the technical verification of a novel method to reverse transcribe and pre-amplify miRNA and mRNA from sample limiting serum research samples using TaqMan® Advanced miRNA cDNA synthesis Kit.

    Based on results from previous studies and unpublished data, Dr. Buchholz’s research group identified a signature of 49 mRNA and 37 miRNA targets that may help to distinguish between benign and malignant pancreatic tissues in the future. In this study these previously identified miRNA and mRNA targets have been used to study expression pattern differences in serum from Normal and Test samples. These miRNA and mRNA targets were spotted on Custom TaqMan array cards to facilitate investigation of 4 samples on each card. Initial results indicate that miRNA and mRNAs can be reliably quantified from a single reverse transcription reaction.

    Learning Objectives:

    • Study of miRNAs in Oncology Gene Expression
    • Understanding the interplay between miRNA and mRNA impacts cancer development and progression
    • Use of Data Analysis tools for Gene Expression studies involving miRNA and mRNA
    • Understanding molecular mechanisms that may potentially have an underlying role in various diseases

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