NOV 15, 2017 9:00 AM PST

WEBINAR: The essential role of miRNA and mRNA on cancer progression and biomarker discovery

Speaker

Abstract

DATE: November 15, 2017
TIME: 09:00am PST, 12:00pm EST, 6:00pm CET

MicroRNAs (miRNA) are a class of small non-coding RNAs (approximately 21 nt long) that bind complementary sequences in target mRNAs to specifically regulate gene expression. miRNAs play important roles in regulation of gene expression during cell development, differentiation, proliferation and apoptosis. Misregulation of miRNAs and their targets has been associated with several diseases including cancer. The interplay between miRNA and mRNA has been found to be important in cancer development and progression. Simultaneous expression studies of miRNA and mRNA can be valuable in understanding molecular mechanisms that may potentially have an underlying role in various diseases. In this webinar, we demonstrate the technical verification of a novel method to reverse transcribe and pre-amplify miRNA and mRNA from sample limiting serum research samples using TaqMan® Advanced miRNA cDNA synthesis Kit.

Based on results from previous studies and unpublished data, Dr. Buchholz’s research group identified a signature of 49 mRNA and 37 miRNA targets that may help to distinguish between benign and malignant pancreatic tissues in the future. In this study these previously identified miRNA and mRNA targets have been used to study expression pattern differences in serum from Normal and Test samples. These miRNA and mRNA targets were spotted on Custom TaqMan array cards to facilitate investigation of 4 samples on each card. Initial results indicate that miRNA and mRNAs can be reliably quantified from a single reverse transcription reaction.

Learning Objectives:

  • Study of miRNAs in Oncology Gene Expression
  • Understanding the interplay between miRNA and mRNA impacts cancer development and progression
  • Use of Data Analysis tools for Gene Expression studies involving miRNA and mRNA
  • Understanding molecular mechanisms that may potentially have an underlying role in various diseases

Show Resources
You May Also Like
MAY 17, 2022 9:00 AM PDT
MAY 17, 2022 9:00 AM PDT
Date: May 17, 2022 Time: 9:00am (PDT), 12:00pm (EDT), 8:00pm (CEST) Gene therapeutics have great potential to treat many severe diseases in an unprecedented, targeted manner. The biopharmace...
APR 26, 2022 7:00 AM PDT
C.E. CREDITS
APR 26, 2022 7:00 AM PDT
Date: April 19, 2022 Time: 7:00am (PDT), 10:00am (EDT), 4:00pm (CEST) High-content (HC) phenotypic profiling approaches are a powerful tool to study the effect of biological, genetic, and ch...
MAR 02, 2022 9:00 AM PST
C.E. CREDITS
MAR 02, 2022 9:00 AM PST
Date: March 02, 2022 Time: 9:00am (PST), 12:00pm (EST) Single cell RNA-seq is known to only capture a small fraction of the transcriptome of each cell. Often, this is due to inherent limitat...
APR 28, 2022 8:00 AM PDT
APR 28, 2022 8:00 AM PDT
Date: April 28, 2022 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) Human pluripotent stem cells (PSCs) and their derivatives hold great potentials in...
MAR 30, 2022 6:00 AM PDT
MAR 30, 2022 6:00 AM PDT
Targeted NGS has been instrumental in helping the healthcare community deliver on the promise of precision medicine. The Ion Torrent Genexus Integrated Sequencer has enabled targeted genomic...
NOV 30, 2021 10:00 AM PST
C.E. CREDITS
NOV 30, 2021 10:00 AM PST
Date: November 30, 2021 Time: 10:00am (PDT), 1:00pm (EDT) The prevalence of thyroid disease worldwide has served as a catalyst for healthcare providers to study various tools and methods to...
NOV 15, 2017 9:00 AM PST

WEBINAR: The essential role of miRNA and mRNA on cancer progression and biomarker discovery



Show Resources
Loading Comments...
Show Resources