MENU

Examining the negative impacts of bioflavonoids on the DNA damage response and DNA repair mechanisms in mouse embryonic stem cells

Speaker

Abstract

Bioflavonoids are dietary agents found in everyday food items such as fruits, vegetables, and legumes. It has been suggested that bioflavonoids be used with chemotherapeutics as an anti-cancer agent due to their anti-inflammatory capabilities and their ability to inhibit the DNA damage response. However most drugs that inhibit the DNA damage response can have secondary effects on normal cells. For instance, chemotherapeutics, such as etoposide, can cause DNA translocations leading to malignant transformations of normal cells to cancer cells. Studies have shown that etoposides and other chemotherapeutics increase the risk of leukemia. Therefore, it is our hypothesis that bioflavonoids inhibit the DNA damage response in normal cells, leading to improper repair of DNA damage, such as chromosomal translocation that can lead to leukemia. To study this we will use mouse embryonic stem cells, to model both acute high-dose exposure and chronic low-dose exposure to bioflavonoids such as genistein, quercetin, luteolin, myricetin etc. After we expose cells to these bioflavonoids, we will be examining them for DNA damage using Immunohistochemistry (IHC) to identify phosphorylation histone variant 2AX, a well-studied marker of DNA damage, with confocal microscopy. We will also be using a translocation reporter construct, which causes cells to produce green fluorescent protein when a translocation has occurred. We have integrated this construct into a mouse embryonic stem cell line, to measure incidences of DNA translocation by flow cytometry. Finally, we will be collecting DNA from these cells for PCR analysis and DNA sequencing to verify any DNA translocations that have occured.


Show Resources
You May Also Like
MAY 11, 2021 10:00 AM PDT
C.E. CREDITS
MAY 11, 2021 10:00 AM PDT
Date: May 11, 2021 Time: 10:00zm PDT Your samples are some of the most valuable assets in the laboratory. After spending countless hours on extraction and preparation, your conclusions could...
SEP 14, 2021 7:00 AM PDT
C.E. CREDITS
SEP 14, 2021 7:00 AM PDT
Date: September 14, 2021 Time: 7am PDT, 10am EDT, 4pm CEST A conventional thermal cycler has long been a commodity product in the lab and end-point PCR techniques can be completed almost wit...
JUN 09, 2021 7:00 AM PDT
C.E. CREDITS
JUN 09, 2021 7:00 AM PDT
Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...
JUL 15, 2021 8:00 AM PDT
C.E. CREDITS
JUL 15, 2021 8:00 AM PDT
Date: July 15, 2021 Time: 8:00am (PDT), 11:00am (EDT) High dimensional full spectrum flow cytometry grants unprecedented access to previously unattainable parameters in cellular biology. Flu...
JUN 24, 2021 10:00 AM PDT
C.E. CREDITS
JUN 24, 2021 10:00 AM PDT
Date: June 24, 2021 Time: 10:00am PDT The Chan Zuckerberg Initiative (CZI) was founded to help solve some of society’s toughest challenges— from eradicating disease and improving...
JUL 15, 2021 9:00 AM PDT
JUL 15, 2021 9:00 AM PDT
Date: July 15, 2021 Time: 9:00am (PDT), 12:00pm (EDT) The Pisces workflow robust, easy-to-use, end-to-end multi-omics solution for highly multiplexed targeted Spatial RNA analysis. VeranomeB...

Examining the negative impacts of bioflavonoids on the DNA damage response and DNA repair mechanisms in mouse embryonic stem cells


No demographic data is available yet for this event.


Show Resources
Loading Comments...
Show Resources