The Expanding Role of Next-Generation DNA Sequencing (NGS) in PJI Diagnosis and Clinical Microbiology - Part II

Date:  November 2, 2020

Time: 8:00am PDT

Timely and accurate detection of an infective organism(s) impacts the treatment course and potentially patient outcomes. Although traditional culture has served as the “gold standard” for the recovery and isolation of potential pathogens, NGS, a culture independent genomic technology, has become attractive to undertake coincidently for the genotypic detection of potential pathogens.  The limitations inherent in traditional microbiological culture techniques—including how biofilm, viable but non-culturable bacteria, and persister cells hinder diagnosis and treatment of these infections—were discussed in detail in Part 1 of this webinar series. Watch On-Demand

NGS has played an important role in the detection of potential microbial pathogens in patients with periprosthetic joint infection (PJI), when infection exists in the form a biofilm and traditional culture fails to isolate the etiological agent in up to 50% of cases. Recent studies have shown that NGS can determine the type of pathogen(s) causing PJI in 90% of the cases when culture was negative. Further, studies have shown that NGS is also extremely useful in urosepsis, neurological infections, and many other fields. 

NGS provides more comprehensive information about the presence of microbial population, results are usually available in <24 hours in most cases and is cost effective. There is ample evidence to support the use of NGS in management of patients with infection.

In this webinar, we will cover:

•    Surgeon’s perspective: Dr Javad Parvizi, a recognized expert in the field of orthopedic infections, will provide his perspective on the use of NGS in orthopedic infections. He will present the data generated over the last four years in using NGS in orthopedics at the Rothman Orthopaedic Institute (Jefferson University Hospitals) and findings of the landmark multi-center study that includes centers such as Cleveland Clinic, Hospital for Special Surgery and so on. 
•    Microbiologist’s perspective: Dr James Snyder, Professor of Pathology and Medical Director of the Clinical Microbiology Laboratory of the University of Louisville Hospital, will talk about their experience of incorporating NGS testing into their microbiology laboratory workflow and lessons learned.  
 

Webinars will be available for unlimited on-demand viewing after live event.

LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.