SEP 27, 2018 6:00 AM PDT

Fighting Against Air: Physiologic Oxygen and Reproducibility

Presented at: Cell Biology 2018
C.E. Credits: P.A.C.E. CE Florida CE
Speaker
  • Chief Scientific Officer, BioSpherix
    Biography
      Alicia D. Henn PhD MBA is Chief Scientific Officer for BioSpherix, Ltd. Previously, Dr. Henn was a researcher at University of Rochester in the Center for Biodefense Immune Modeling working on influenza vaccine biology. Before that, she was an industry immuno-oncology researcher discovering novel breast tumor antigens at Vaccinex, LLC. As CSO at BioSpherix, she established the company's first comprehensive scientific program, collaborating with researchers in both academia and industry to advance cellular therapies and stem cell research. Dr. Henn works quite closely with sales and marketing teams to align internal company research with customer interests. She provides advanced training and scientific support to customers transitioning to Cytocentric Systems and is active in building the company's first applications support team. Dr. Henn owns the In Vitro Reproducibility group on LinkedIn. She also writes the Cytocentric Blog on the company website, promoting physiologically relevant environments for better scientific reproducibility and cell performance.

    Abstract

    Room air conditions are hypothermic, hypocapnic and hyperphysioxic from the cells’ point of view. Yet we stress cells with these uncontrolled conditions routinely. It is no wonder that we see problems with the reproducibility and translatability of in vitro cell data. The nascent field of immunometabolism is revealing that metabolic processes, responsive to oxygen, do more than just support critical cell processes like proliferation and differentiation, but actually control them. In this talk we will dive into basic environmental conditions for cells in research, particularly physiologic oxygen. We will talk about the physiology behind different cellular oxygen levels in vivo and how to match those conditions in vitro for both cell incubation and cell handling.  We will also present data showing that constant, physiologically relevant conditions for both incubation and cell handling can improve cell growth in vitro. It is all part of establishing basic cell conditions that are Cytocentric, putting the cells’ needs first. 
     

    Learning Objectives: 

    1. Participants will be able to discuss physiologic oxygen knowledgably, both in vivo and in vitro.
    2. Participants will be able to distinguish between Cytocentric and Peoplecentric cell culture conditions.


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