Why did we change focus from the 3Rs to Systematic Reviews? Questionnaires on the 3Rs have revealed that the 3R search gets no high priority and the 3R search process is never included in funding proposals (Leenaars et al. ATLA 2009, Van Luijk et al. ATLA 2011). As there are more than 100 databases and websites dealing with the 3Rs, each having a different content and buildup and method of search (Leenaars et al. ATLA 2009), making the 3R search complicated and ineffective, we have shifted focus to Systematic Reviews (SR). This is a succesful and well-developed and accepted method originating from evidence-based medicine (Cochrane Collaboration). SR can contribute to better quality science, better patient safety ánd the implementation of the 3Rs (Hooijmans & Ritskes-Hoitinga 2013; Ritskes-Hoitinga et al. 2014). Therefore we have founded SYRCLE, Systematic Review Center for Laboratory animal Experimentation. SYRCLE develops tools and guidelines to make this method just as easily accessible and applicable in laboratory animal science as in the medical field (Hooijmans et al. 2010, 2011; De Vries et al. 2011; 2014 Leenaars et al. 2011.) SYRCLE develops and offers teaching, e-learning, workshops and coaching (see www.SYRCLE.nl). In my presentation I will discuss the methodology and value of SR of animal studies and present various practical examples on the evidence-based choice of animal models and on the translational value.