JUN 10, 2021 10:00 AM PDT
Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar

GABA-receptive microglia selectively sculpt developing inhibitory circuits

Sponsored by: Vizgen
C.E. Credits: P.A.C.E. CE Florida CE
Speakers
  • Klarman Cell Observatory, Broad Institute of Harvard and MIT
    Biography
      After completing an engineering degree at Ecole Polytechnique, Paris, in 2012, Loic completed a master’s degree working on tissue engineering at Ecole Polytechnique of Montreal, then a phD In biomedical engineering at University of Montreal in 2019. His graduate work focused on developing optical approaches to target live cells chosen on visible criteria to both tag them without knowledge of their surface biochemistry and isolate them. Loic joined the broad institute in November 2019 and his work primarily focuses on applying optical approaches such as MERFISH to large-scale genomic screens.
    • Harvard Medical School and Broad Institute of Harvard and MIT
      Biography
        Emilia started her studies in 2007 at the Sapienza University of Rome and was awarded a B.Sc. in Biological Sciences and a M.Sc. in Neurobiology. In 2011, she received a predoctoral fellowship from the Spanish National Research Council and joined the group of Beatriz Rico at the Institute of Neuroscience in Alicante (Spain). She moved with that group to the Centre for Developmental Neurobiology at King’s College London in 2014 where she terminated her doctoral training. Her graduate research work focused on the cellular and molecular mechanisms of inhibitory circuit development and plasticity in the cerebral cortex. She then received an EMBO postdoctoral fellowship to work on the cellular and molecular mechanism of microglia-inhibitory synapse interactions during development. Since 2017, she is a postdoctoral associate in Gord Fishell’s laboratory at Harvard Medical School and the Broad Institute. Over the years, she was awarded numerous prizes such as the Beddington Medal from the British Society for Developmental Biology and the Krieg Cortical Kudos Scholar Award from the Cajal Club.

      Abstract
      Date:  June 10, 2021
      Time: 10:00am (PDT),  1:00pm (EDT)
       
      Microglia, the primary brain macrophages, regulate a plethora of processes that impact the organization of neural circuits, including synapse pruning. Whether microglia are generic effectors of synapse pruning or if specialized microglia are able to discriminate between distinct synapse types is unknown. We found that GABA-receptive microglia selectively interact with inhibitory cortical synapses during a critical window of mouse postnatal development. Single cell RNA-seq and MERFISH profiling showed that ablation of microglial GABAB receptors cell-autonomously alters a transcriptional synapse remodeling program. As a result, loss of GABABRs within microglia alters inhibitory connectivity without impacting excitatory synapses and leads to behavioral abnormalities. These findings demonstrate that distinct microglia differentially engage with specific synapse types during development.
       
      Learning Objectives
      • Explain how MERFISH profiling can complement scRNA-seq data
      • Demonstrate how the selective communication between matching cell types regulates brain wiring
      • Reveal how early developmental events influence adult behavior
       
       
      Webinars will be available for unlimited on-demand viewing after live event.
       
      LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.

      Show Resources
      You May Also Like
      MAY 11, 2021 10:00 AM PDT
      C.E. CREDITS
      MAY 11, 2021 10:00 AM PDT
      Date: May 11, 2021 Time: 10:00zm PDT Your samples are some of the most valuable assets in the laboratory. After spending countless hours on extraction and preparation, your conclusions could...
      JUN 09, 2021 7:00 AM PDT
      Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
      C.E. CREDITS
      JUN 09, 2021 7:00 AM PDT
      Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
      Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...
      DEC 02, 2020 8:00 AM PST
      C.E. CREDITS
      DEC 02, 2020 8:00 AM PST
      DATE: December 2nd, 2020 TIME: 08:00am PDT, 11:00pm EDT Bioreactors and shakers are used to cultivate microorganisms, plant, insect, and mammalian cells in different volumes. Upscaling of pr...
      DEC 16, 2020 8:00 AM PST
      C.E. CREDITS
      DEC 16, 2020 8:00 AM PST
      Date: December 16, 2020 Time: 8:00am (PST), 11:00am (EST) Molecular imaging of living specimens offers a means to draw upon the growing body of high-throughput molecular data to better under...
      MAR 16, 2021 10:00 AM PDT
      C.E. CREDITS
      MAR 16, 2021 10:00 AM PDT
      Date: March 16, 2021 Time: 10:00am (PST) Scientific progress and breakthroughs today are often too expensive for most institutions to acquire. Each year, the National Institutes of Health (N...
      APR 21, 2021 5:00 PM CEST
      APR 21, 2021 5:00 PM CEST
      Date: April 21, 2021 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) Spatial Answers Trilogy - Spatial Answers in Immunology Immunology Researchers share their Spatial Discoveries in SARS-C...
      Loading Comments...
      Show Resources
      Event Countdown
      • 0 Days
      • 0 Hours
      • 0 Minutes
      • 0 Seconds
      Attendees
      • See more