MENU

Gaining critical insights for drug discovery applications using high-content cell analysis platforms

Speaker
  • Senior Research Scientist, AstraZeneca
    Biography
      David Baker, Ph.D. has worked at AstraZeneca for three years following his Ph.D. in cellular neuroscience at the University of Sheffield. David works within the Cellular Assay Development group within Discovery Biology, where his main role is to develop assays for oncology and neuroscience drug discovery projects that enable screening of hundreds to millions of compounds. In addition to development of plate-based and high-content cellular assays, David has been heavily involved in setting up the immuno-oncology capability utilizing high-throughput flow cytometry, developing assays to look at T cell proliferation, activation and killing for use in AstraZenecaís immuno-oncology projects.

    Abstract

    Gain insights from an assay development expert as Dr. David Baker, of AstraZeneca, discusses how you can enable rapid measurement of cell proliferation, transfection efficiency, cell confluence and other cellular characteristics for assay optimization using live-cell imaging and high throughput flow cytometry.

    Establishing the characteristics of cell models is a key component of assay optimization processes performed at AstraZeneca. Learn how to use longitudinal live-cell imaging assays to characterize cells for assay development and assay endpoints. Examples will include how to measure cell confluence and assess the proliferation rate of different cell types to establish optimal assay conditions to screen for cytostatic or cytotoxic compounds in cancer cell lines.

    In addition to analysis of cell phenotype using imaging platforms, high-throughput flow cytometry is also being used to gain insights into cell behavior, especially in the immuno-oncology field. Harnessing the immune system to combat cancer is a key interest area for AstraZeneca, and characterizing treatment effects upon immune cell phenotype is a key piece of data in drug discovery workflows. Learn how high-throughput flow cytometry is being used to enable rapid analysis of cells and beads to measure cytokine levels, cell proliferation and cytolytic activity of primary human immune cells.

    In this webinar Dr. David Baker will discuss how to assess:

    • cell proliferation, confluence and transfection efficiency using longitudinal live-cell imaging.
    • cytokine release, proliferation and cytolytic activity of immune cells using high-throughput flow cytometry.

    Show Resources
    You May Also Like
    MAY 11, 2021 10:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    MAY 11, 2021 10:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    Date: May 11, 2021 Time: 10:00zm PDT Your samples are some of the most valuable assets in the laboratory. After spending countless hours on extraction and preparation, your conclusions could...
    JUN 09, 2021 7:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    JUN 09, 2021 7:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...
    NOV 16, 2020 8:00 AM PST
    C.E. CREDITS
    NOV 16, 2020 8:00 AM PST
    Date: November 16, 2020 Time: 8:00am (PST), 11:00am (EST) CRISPR screening has become the prime discovery tool in modern biomedical research and drug discovery. At the same time, most screen...
    DEC 02, 2020 8:00 AM PST
    C.E. CREDITS
    DEC 02, 2020 8:00 AM PST
    DATE: December 2nd, 2020 TIME: 08:00am PDT, 11:00pm EDT Bioreactors and shakers are used to cultivate microorganisms, plant, insect, and mammalian cells in different volumes. Upscaling of pr...
    JAN 21, 2021 8:00 AM PST
    JAN 21, 2021 8:00 AM PST
    Date: January 21, 2021 Time: 8:00am (PST), 11:00am (EST) Today, critical reagent characterization is a key component in the overall workflow to establish robust ligand binding assays (e.g.,...
    APR 01, 2021 8:00 AM PDT
    C.E. CREDITS
    APR 01, 2021 8:00 AM PDT
    Date: April 01, 2021 Time: 8:00am (PST), 11:00am (EST) Generating therapeutic antibodies is far more challenging than obtaining antibodies that merely recognize their targets. Engineering po...

    Gaining critical insights for drug discovery applications using high-content cell analysis platforms


    No demographic data is available yet for this event.


    Show Resources
    Loading Comments...
    Show Resources