OCT 03, 2019 7:30 AM PDT

Keynote Presentation: CRISPR/Cas9 genome editing in mouse and rat: The PHENOMIN-ICS experience

Presented at: CRISPR 2019
C.E. Credits: P.A.C.E. CE Florida CE
Speaker

Abstract

CRISPR/Cas9 technology has become very popular in the last 5 years and has profoundly changed our way to do genetically engineered animals. I will present the latest development in genome editing at PHENOMIN-ICS.  From  the easy high throughput KO production for the International Mouse Phenotyping consortium  (https://www.mousephenotype.org/) to the generation of complex models, different examples (from simple point mutations to conditional knock-outs) will be shown and discussed.  The benefits of oocyte electroporation, which is much more efficient than microinjection and has allowed to reduce the number of animal needed for all experiments, will be presented. The pertinence of the use of CRISPR base editing (cytidine deaminase) for specific mutations was assessed and the results of our pilot experiment will be shown. And finally, the advantages of the use of embryonic stem cells (with the help of CRISPR) for genetic modifications larger than small knock-ins will be presented.


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OCT 03, 2019 7:30 AM PDT

Keynote Presentation: CRISPR/Cas9 genome editing in mouse and rat: The PHENOMIN-ICS experience

Presented at: CRISPR 2019
C.E. Credits: P.A.C.E. CE Florida CE

Specialty

Bioinformatics

Cell Culture

Immunology

Molecular Biology

Biotechnology

Animal Research

Immunity

Gene

Biomarkers

Animals

Bacteria

Dna Sequencing

Staphylococcus (Staph Infection)

Clinical Diagnostics

Mass Cytometry

Geography

North America30%

Africa30%

Asia20%

Europe20%

Registration Source

Website Visitors100%

Job Title

Research Scientist43%

Student29%

Educator/Faculty14%

Scientist14%

Organization

Academic Institution60%

Research Institute20%

Industrial Company10%

Biotech Company10%


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