Myeloid neoplasms are a group of heterogeneus disorders originating from the neoplastic transformation of a pluripotent hematopoietic progenitor cell. Following the acquisition of somatic mutations there is an abnormal proliferation of hematopoietic cells from one or more cell lineages with the predominance of the neoplastic clone over the normal cells. Myeloid disorders are characterized by a high molecular complexity and by the presence of subclonal mutations occurring in subclones of variable size. The “classic” molecular biology techniques present several limits in terms of sensibility, sensitivity and time consuming. Next generation sequencing (NGS) have become fundamental for the in-depth study of molecular complexity and translational research in these diseases. Moreover the access to semi-automated platform and dedicated myeloid gene panels, represent an efficient and reliable opportunity for researchers to improve the quality of molecular data analysis.
1. Evolution of technology in molecular biology
2. Next generation utility in myeloid neoplasm
3. Impact of NGS on translational research