MAR 15, 2017 1:30 PM PDT

New generation open source miniaturized microscopes for network dynamic imaging in freely moving mice

Presented at: Neuroscience 2017
Speaker
  • Assistant Professor, Neurology, Brain Research Institute Member, Neuroscience GPB Home Area
    Biography
      Dr. Golshani obtained his MD/PhD from UC Irvine and UC Davis where he trained under the mentorship of Dr. Edward G. Jones on the development of corticothalamic synapse. He then completed his Neurology residency at UCLA and obtained postdoctoral training with Dr. Felix Schweizer and Dr. Guoping Fan where he studied the role of DNA methylation in development of cortical circuits. He then became faculty and in collaboration with Dr. Portera-Cailliau and Dr. Stelios Smirnakis studied the developmental desynchronization of internally generated activity in the cerebral cortex. His independent laboratory now investigates how cortical microcircuits in the awake behaving animal encode sensory input and how disorders such as autism and developmental epilepsies disrupt functional cortical connectivity.

    Abstract

    Miniaturized microscopes in combination with genetically encoded calcium indicators now allow recordings of activity from hundreds of neurons simultaneously in freely moving animals. We are building new generation open source miniaturized microscopes with expanded capabilities.  First, we have built and wireless miniaturized microscopes allow unfettered recordings for over 15 minutes.  These microscopes will allow recordings from a number of behaviors that have been thus far been impossible. For example, the microscopes will allow recordings from mice behaving in very large naturalistic arenas or complex tunnel complexes.  Second, we are building microscopes with combined optogenetics and calcium imaging capabilities, so that the activity of neurons can be manipulated while recording from the same group of cells.  Third, we are developing microscopes with combined electrophysiology and imaging capabilities to correlate imaging findings with electrophysiological signatures.  Finally, our first generation microscopes have already been released into our open-source wiki (miniscope.org) and over 150 labs are currently building and using the microscopes.  As the new generation microscopes are tested and optimized they will also be released to the community.


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