The use of pluripotent stem cell-derived cell types for disease modeling, drug screening and regenerative medicine is an exciting area of activity in health research. Prior to the availability of pluripotent stem cells and differentiation methods, relevant and affected primary cells were difficult to obtain and frequently only accessible post-mortem. Additionally, the recent development of CRISPR/Cas9 based genome editing methods allow the creation of isogenic (matched) disease and control lines that differ only at a specific, disease-relevant locus, or to insert reporter constructs into PSC lines such that the reporter may be used to study disease biology in relevant cell types after differentiation. Recently, CCRM has created a standard protocol for efficient genome engineering in human pluripotent stem cells (hPSC). This talk will describe our protocol, and its application to the creation of a cell line that contains a voltage-sensitive fluorescent reporter that will be used to study cardiac function and disease.
Cancer Research
Cell Biology
Molecular Biology
Immunology
Cell Culture
Biotechnology
Genetics
Molecular Diagnostics
Clinical Diagnostics
Cancer Therapeutics
Biochemistry
Cancer Diagnostics
Biomarkers
Dna
Biomedical Engineering
North America54%
Europe25%
Asia18%
South America2%
Website Visitors100%
Student25%
Research Scientist17%
Scientist10%
Post Doc8%
Lab Management8%
Medical Laboratory Technician6%
Medical Doctor/Specialist6%
Executive6%
Educator/Faculty6%
Facility/Department Manager4%
Engineer2%
Hospital11%
Academic Institution11%
Medical School7%
Research Institute5%
Clinical Laboratory4%
Life Science Company4%
Pharmaceutical Company4%
Biotech Company4%
Non-Profit Organization2%
Industrial Company2%
Media2%
Ambulatory Care2%
Medical Center2%
Other5%