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FEB 21, 2017 11:00 AM PST

Genome Engineering to Introduce a Fluorescent Reporter into Human Pluripotent Stem Cells to Study Cardiac Disease

Speaker

Abstract

The use of pluripotent stem cell-derived cell types for disease modeling, drug screening and regenerative medicine is an exciting area of activity in health research. Prior to the availability of pluripotent stem cells and differentiation methods, relevant and affected primary cells were difficult to obtain and frequently only accessible post-mortem. Additionally, the recent development of CRISPR/Cas9 based genome editing methods allow the creation of isogenic (matched) disease and control lines that differ only at a specific, disease-relevant locus, or to insert reporter constructs into PSC lines such that the reporter may be used to study disease biology in relevant cell types after differentiation. Recently, CCRM has created a standard protocol for efficient genome engineering in human pluripotent stem cells (hPSC). This talk will describe our protocol, and its application to the creation of a cell line that contains a voltage-sensitive fluorescent reporter that will be used to study cardiac function and disease.


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FEB 21, 2017 11:00 AM PST

Genome Engineering to Introduce a Fluorescent Reporter into Human Pluripotent Stem Cells to Study Cardiac Disease


Specialty

Cancer Research

Cell Biology

Molecular Biology

Immunology

Cell Culture

Genetics

Biotechnology

Molecular Diagnostics

Biochemistry

Pcr/rt-Pcr/real-Time Pcr

Cancer Diagnostics

Gene Expression

Dna

Clinical Diagnostics

Cancer Therapeutics

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North America54%

Europe25%

Asia18%

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Medical Laboratory Technician6%

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Facility/Department Manager4%

Engineer2%

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Academic Institution11%

Medical School7%

Research Institute5%

Clinical Laboratory4%

Life Science Company4%

Pharmaceutical Company4%

Biotech Company4%

Non-Profit Organization2%

Industrial Company2%

Media2%

Ambulatory Care2%

Medical Center2%

Other5%


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