Development Scientist, CCRM
BIOGRAPHY
FEB 21, 2017 11:00 AM PST
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Genome Engineering to Introduce a Fluorescent Reporter into Human Pluripotent Stem Cells to Study Cardiac Disease
Presented at:
4th Annual 24 Hours of Stem Cells™ virtual event
Speaker
Abstract
The use of pluripotent stem cell-derived cell types for disease modeling, drug screening and regenerative medicine is an exciting area of activity in health research. Prior to the availability of pluripotent stem cells and differentiation methods, relevant and affected primary cells were difficult to obtain and frequently only accessible post-mortem. Additionally, the recent development of CRISPR/Cas9 based genome editing methods allow the creation of isogenic (matched) disease and control lines that differ only at a specific, disease-relevant locus, or to insert reporter constructs into PSC lines such that the reporter may be used to study disease biology in relevant cell types after differentiation. Recently, CCRM has created a standard protocol for efficient genome engineering in human pluripotent stem cells (hPSC). This talk will describe our protocol, and its application to the creation of a cell line that contains a voltage-sensitive fluorescent reporter that will be used to study cardiac function and disease.
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FEB 21, 2017 11:00 AM PST
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Genome Engineering to Introduce a Fluorescent Reporter into Human Pluripotent Stem Cells to Study Cardiac Disease
Presented at:
4th Annual 24 Hours of Stem Cells™ virtual event
Specialty
Cancer Research
Cell Biology
Molecular Biology
Immunology
Cell Culture
Genetics
Biotechnology
Molecular Diagnostics
Biochemistry
Pcr/rt-Pcr/real-Time Pcr
Cancer Diagnostics
Gene Expression
Dna
Clinical Diagnostics
Cancer Therapeutics
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North America54%
Europe25%
Asia18%
South America2%
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Website Visitors100%
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Research Scientist17%
Scientist10%
Post Doc8%
Lab Management8%
Medical Laboratory Technician6%
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Other5%
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