Webinars

FEB 21, 2017 11:00 AM PST

Genome Engineering to Introduce a Fluorescent Reporter into Human Pluripotent Stem Cells to Study Cardiac Disease

Presented at: 4th Annual 24 Hours of Stem Cells™ virtual event

Speaker

Lise Munsie, PhD
Development Scientist, CCRM
Biography
Dr. Lise Munsie currently holds the position of Development Scientist at CCRM. She obtained her PhD in the department of Biochemistry and Biomedical Science at McMaster University and held a 3-year postdoctoral fellowship at the University of British Columbia (UBC), jointly between the department of Medical Genetics and the department of Neuroscience. At CCRM, a leader in developing and commercializing regenerative medicine technologies, and cell and gene therapies, Lise oversees a variety of projects related to iPSC derivation, directed differentiation and genome engineering.

Abstract

The use of pluripotent stem cell-derived cell types for disease modeling, drug screening and regenerative medicine is an exciting area of activity in health research. Prior to the availability of pluripotent stem cells and differentiation methods, relevant and affected primary cells were difficult to obtain and frequently only accessible post-mortem. Additionally, the recent development of CRISPR/Cas9 based genome editing methods allow the creation of isogenic (matched) disease and control lines that differ only at a specific, disease-relevant locus, or to insert reporter constructs into PSC lines such that the reporter may be used to study disease biology in relevant cell types after differentiation. Recently, CCRM has created a standard protocol for efficient genome engineering in human pluripotent stem cells (hPSC). This talk will describe our protocol, and its application to the creation of a cell line that contains a voltage-sensitive fluorescent reporter that will be used to study cardiac function and disease.

Show Resources

You May Also Like

OCT 08, 2020 7:00 AM PDT

C.E. CREDITS

OCT 08, 2020 7:00 AM PDT

Protect your cells with proper pipetting - How liquid handling...

DATE: October 8, 2020 TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop thinking about ho...

DEC 02, 2020 8:00 AM PST

C.E. CREDITS

DEC 02, 2020 8:00 AM PST

Optimize your bioprocess - From shaker to bioreactor

DATE: December 2nd, 2020 TIME: 08:00am PDT, 11:00pm EDT Bioreactors and shakers are used to cultivate microorganisms, plant, insect, and mammalian cells in different volumes. Upscaling of pr...

NOV 16, 2020 8:00 AM PST

C.E. CREDITS

NOV 16, 2020 8:00 AM PST

Targeted single-cell RNA sequencing enables genome-scale CRISPR...

Date: November 16, 2020 Time: 8:00am (PST), 11:00am (EST) CRISPR screening has become the prime discovery tool in modern biomedical research and drug discovery. At the same time, most screen...

Speaker: Wieland Keilholz, Ph.D. , Pawel Zajac, Ph.D. , Dr. Tilmann Buerckstuemmer

Sponsored By: Illumina

DEC 16, 2020 8:00 AM PST

C.E. CREDITS

DEC 16, 2020 8:00 AM PST

Adding Dimensions to Multiplex Molecular Imaging

Date: December 16, 2020 Time: 8:00am (PST), 11:00am (EST) Molecular imaging of living specimens offers a means to draw upon the growing body of high-throughput molecular data to better under...

DEC 09, 2020 9:00 AM PST

C.E. CREDITS

DEC 09, 2020 9:00 AM PST

Human hematopoietic stem cell research made smart and easy

DATE: December 09, 2020 TIME: 09:00am PST Hematopoietic stem cells (HSCs) ensure homeostasis of blood throughout life. In this webinar, we share insights into the smart and easy isolation, c...

SEP 10, 2020 9:00 AM PDT

C.E. CREDITS

SEP 10, 2020 9:00 AM PDT

An Insider's Look: How One Parameter Can Optimize Key Phases of...

Date: September 10, 2020 Time: 9:00am (PDT), 12:00pm (EDT) Osmolality testing is relevant throughout the entire bioprocessing workflow. As customers look to refine mAb and gene therapy workf...