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MAY 09, 2017 7:00 PM PDT

Gibco™ ExpiCHO哺乳动物瞬时蛋白表达系统的最新研发进展

Speaker
  • Sr. Staff Scientist, Cell Biology, Thermo Fisher Scientific
    Biography
      Chao Yan Liu, is a Senior Staff Scientist, at Cell Biology of the Life Sciences Solutions Group - now part of Thermo Fisher Scientific - at the Frederick (MD - USA) location. She received the M.D. degree from the Hebei Medical University (Hebei, China) and the Master degree in Pathology from the Academy of Military Medical Sciences in Beijing, China. After being very active for more than ten years in academic research in the field of cellular biology and immunology, in 2005 she joined the Research and Development department of the former Life Technologies. She has made great contributions to more than ten of the Gibco brand Cell Culture Essential products including AlgiMatrix 3D Culture System, OpTmizer CTS T-Cell Expansion SFM, TrypLE Select Enzyme, ES Cell Qualified FBS, GlutaMAX media supplement, FreeStyle F17 Expression Medium and CD FortiCHO Medium. She has extensive experiences in mammalian cell culture, serum and protein free, chemical defined medium formation as well as protein expression. In the past five years, she served as leading scientist to focus on the development of two novel transient transfection systems, Expi293™ and ExpiCHO Expression Systems.

      公司细胞生物学部门的高级研究员,在美国马里兰州弗雷德里克工作。Chao Yan Liu女士拥有河北医科大学的医学学士学位和军事医学科学院的病理学硕士学位,先后在威斯康辛州血液中心的血液研究所以及纽约州立大学水牛城分校从事细胞生物学和免疫学领域的学术研究工作。Chao Yan Liu女士于2005年加入原Life Technologies公司研发部,对Gibco™ 品牌下十余款细胞培养产品的研发做出了卓越贡献,包括AlgiMatrix™ 3D培养系统、OpTmizer™ CTS™ T细胞扩增无血清培养基、TrypLE™ Select酶、适用于胚胎干细胞的FBS、MesenPRO RS™ 细胞培养基、GlutaMAX™ 培养基添加剂、FreeStyle™ F17表达培养基和CD FortiCHO™ 培养基,在哺乳动物细胞培养,尤其是化学成分确定的无血清和无蛋白培养以及重组蛋白表达方面积累了丰富的经验。最近五年,她作为瞬时蛋白表达课题研发的技术负责人致力于两种新型哺乳动物瞬时蛋白表达系统--Expi293™和ExpiCHO™的研究开发并取得了突破性进展。

    Abstract

    Gibco™ ExpiCHO是一款全新优化的瞬时重组蛋白表达系统,可以在瞬转中获得极高的蛋白产量,为临床新药和诊断试剂的研发及快速筛选提供了高效便捷的最佳手段。一些难以在传统HEK293细胞中表达的蛋白亦可在ExpiCHO系统中获得高水平表达。正是基于ExpiCHO系统在CHO细胞重组蛋白瞬时表达方面的突破性进展,该系统作为不可或缺的革命性工具,目前己成为世界上众多生物制药公司创制和生产最具天然结构及活性蛋白的首选表达平台。

     

    在今天的报告中我们将与同道们共同分享ExpiCHO系统应用的最新进展,包括系统扩展性,操作技巧及常见问题指南,以期帮助广大用户全面掌握蛋白瞬时表达最优条件,满足不同规模高效表达的生产需求。


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