FEB 27, 2019 3:00 PM PST

Do You Have the Gut-on-a-Chip?

Presented at: Drug Discovery 2019
C.E. Credits: P.A.C.E. CE Florida CE
Speaker
  • Scientist of Application Development, Mimetas - The Organ on a Chip Company
    Biography
      Elena Naumovska works as a R&D Scientist at Mimetas focusing in development of organotypic intestinal tissue models that will aide the toxicity evaluation and drug screening processes.
      She finished her undergraduate studies at the Sofia University "St. Kliment Ohridski", Bulgaria with a Major in Molecular Biology and master studies in Molecular and Cellular Biology at the Uppsala University, Sweden. Her work has been in different fields but mostly on interdisciplinary projects raging from fusogenic liposomes and cancer therapy, directed differentiation of embryonic (ES) and induced pluripotent stem cells (iPSC), cancer metabolomics and venous thrombosis.

      In her current position she is also a part of initial training network ITN-MIMIC and working to finish an industrial doctorate in connection to the University of Sheffield, UK later this year.

    Abstract

    In-vitro epithelial barrier models that are more representative of in-vivo tissues are urgently needed. Here we present extracellular matrix-supported intestinal tubules in a perfused microfluidic platform. These intestinal tubules exhibiting tissue (polarization) markers ErbB1, ErBb2, Ezrin, Glut-2, MRP2 and ZO-1, and crypt-like morphology. Drug-induced toxicity was assessed by apical exposure of the intestinal barriers to model compounds. This microfluidic platform is readily implemented in routine laboratories and allows for recording of response to pharmacological stimuli in real-time using automated imaging techniques. The co-culture capabilities of the platform can be explored to create complex tissue configurations, for example by incorporating mesenchymal and immune cells in the ECM adjacent to the epithelial tubes. In conclusion, intestinal tubules cultured in the OrganoPlate® are suitable for high-throughput toxicity screening, trans-epithelial transport studies, and complex co-culture models to recreate an in vivo-like microenvironment.

    Learning Objectives: 

    1. Identify challenges in standard in-vitro models 
    2. Be able describe the method of seeding, growing and investigating intestinal tubular structures on the OrganoPlate® format 
    3. Be able to translate this knowledge in generating high-throughput studies for drug development and discovery


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