FEB 27, 2019 03:00 PM PST

Do You Have the Gut-on-a-Chip?

C.E. CREDITS: P.A.C.E. CE | Florida CE
Speakers
  • Scientist of Application Development, Mimetas - The Organ on a Chip Company
    Biography
      Elena Naumovska works as a R&D Scientist at Mimetas focusing in development of organotypic intestinal tissue models that will aide the toxicity evaluation and drug screening processes.
      She finished her undergraduate studies at the Sofia University "St. Kliment Ohridski", Bulgaria with a Major in Molecular Biology and master studies in Molecular and Cellular Biology at the Uppsala University, Sweden. Her work has been in different fields but mostly on interdisciplinary projects raging from fusogenic liposomes and cancer therapy, directed differentiation of embryonic (ES) and induced pluripotent stem cells (iPSC), cancer metabolomics and venous thrombosis.

      In her current position she is also a part of initial training network ITN-MIMIC and working to finish an industrial doctorate in connection to the University of Sheffield, UK later this year.

    Abstract:

    In-vitro epithelial barrier models that are more representative of in-vivo tissues are urgently needed. Here we present extracellular matrix-supported intestinal tubules in a perfused microfluidic platform. These intestinal tubules exhibiting tissue (polarization) markers ErbB1, ErBb2, Ezrin, Glut-2, MRP2 and ZO-1, and crypt-like morphology. Drug-induced toxicity was assessed by apical exposure of the intestinal barriers to model compounds. This microfluidic platform is readily implemented in routine laboratories and allows for recording of response to pharmacological stimuli in real-time using automated imaging techniques. The co-culture capabilities of the platform can be explored to create complex tissue configurations, for example by incorporating mesenchymal and immune cells in the ECM adjacent to the epithelial tubes. In conclusion, intestinal tubules cultured in the OrganoPlate® are suitable for high-throughput toxicity screening, trans-epithelial transport studies, and complex co-culture models to recreate an in vivo-like microenvironment.

    Learning Objectives: 

    1. Identify challenges in standard in-vitro models 
    2. Be able describe the method of seeding, growing and investigating intestinal tubular structures on the OrganoPlate® format 
    3. Be able to translate this knowledge in generating high-throughput studies for drug development and discovery


    Show Resources
    You May Also Like
    AUG 27, 2019 09:00 AM PDT
    C.E. CREDITS
    AUG 27, 2019 09:00 AM PDT
    DATE: August 27, 2019 TIME: 9:00am PDT, 12:00pm EDT Immunotherapies targeting PD-1 or PD-L1 have proven remarkably effective for treating cancer in some patients, with considerabl...
    SEP 05, 2019 04:00 PM CEST
    C.E. CREDITS
    SEP 05, 2019 04:00 PM CEST
    DATE: September 5, 2019TIME: 7:00am PT, 10:00am ET, 4:00pm CEST PCR (Polymerase Chain Reaction) has gone through a massive evolution since its development in 1983. Besides it...
    OCT 02, 2019 11:00 AM PDT
    OCT 02, 2019 11:00 AM PDT
    DATE: October 2, 2019TIME: 11:00am PDT, 2:00pm EDT Ditch the Excel spreadsheets and manage your molecular workflows entirely in your LIMS Achieve configuration of molecular workf...
    JUN 19, 2019 10:00 AM PDT
    JUN 19, 2019 10:00 AM PDT
    DATE: June 19, 2019TIME: 10:00am PDT, 1:00pm EDT As we develop new methods to create more biologically relevant models for research in understanding disease etiology and in...
    NOV 18, 2019 07:00 AM PST
    C.E. CREDITS
    NOV 18, 2019 07:00 AM PST
    DATE: November 18, 2019TIME: 7:00am PST, 11:00am EST, 4:00pm CEWT How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop th...
    AUG 14, 2019 09:00 AM PDT
    C.E. CREDITS
    AUG 14, 2019 09:00 AM PDT
    DATE: August 14, 2019TIME: 9:00am PT. 12:00pm ET Implementation of Lean Methodology in Biobanking Operations to standardize sample management processes is effective. Each proce...
    Loading Comments...
    Show Resources