MAY 14, 2020 7:05 PM EDT

Haemato-Oncology Applications of BCR Immune Repertoire Sequencing

Speaker

Abstract

B-cell lymphomas and leukemias originate from the malignant transformation and proliferation of one or a small number of B cells. Since each B-cell expresses distinct B-cell receptors (BCRs) on its surface, potential malignant clones of interest can be identified and measured by the unique BCR sequences from the original cell. These B-cell transformations are an important focus area for ongoing translational and clinical research.

Today, next-generation sequencing (NGS) of the BCR repertoire is being increasingly adopted for hemato-oncology research due to the significant advantages it offers over traditional methods like Sanger Sequencing, PCR and flow cytometry. Comparatively, NGS provides ultra-high sensitivity, lower limits of detection (LOD), and greater flexibility to multiplex. In this webinar, we’ll explore a range of hemato-oncology applications for sequencing of the BCR repertoire. NGS-based clonality testing is becoming an essential method of analyzing hematolymphoid proliferations because it allows researchers to simultaneously sequence the malignant clone and evaluate the evolving clonal landscape. The sensitivity of NGS is also well suited for minimal residual disease research, enabling rare clone detection from low frequencies within the cell population. Moreover, NGS can be used to asses somatic hypermutation within the rearranged IGH/BCR gene, an important biomarker associated with Chronic Lymphocytic Leukemia samples.

 

Learning Objectives:

1. Understand the practical & technical advantages of NGS compared to traditional methodologies for BCR IGH analysis 

2. Evaluate NGS hemato-oncology research applications, including clonality assessment, somatic hypermutaion analysis, and minimal residual disease research 

3. Define how NGS can aid in resolution of challenging polyclonal samples


Show Resources
You May Also Like
OCT 11, 2022 8:00 AM PDT
C.E. CREDITS
OCT 11, 2022 8:00 AM PDT
Date: October 11, 2022 Time: 8:00am (PDT), 11:00pm (EDT), 5:00pm (CEST) Multiomic profiling of cell populations at single-cell resolution is revolutionizing scientists’ understanding o...
JUN 21, 2022 6:00 AM PDT
JUN 21, 2022 6:00 AM PDT
Date: June 21, 2022 Time: 6:00am (PDT), 9:00am (EDT), 3:00pm (CEST) The global understanding and practice of medicine is currently undergoing a revolutionary change. This shift to precision...
JUN 28, 2022 7:00 AM PDT
JUN 28, 2022 7:00 AM PDT
Date: June 28, 2022 Time: 3:00pm (BST), 4:00pm (CET), 9:00am (CST), 7am (PST) Light-sheet microscopy is an extremely versatile imaging technique with a vast range of implementations that are...
AUG 16, 2022 11:00 AM PDT
AUG 16, 2022 11:00 AM PDT
Date: August 16, 2022 Time: 11:00am (PDT), 2:00pm (EDT), 8:00pm (CEST) Understanding genomic variation in the context of cancer is paramount to identifying disease drivers and informing pers...
AUG 24, 2022 7:00 AM PDT
AUG 24, 2022 7:00 AM PDT
Date: August 24, 2022 Time: 7:00am (PDT), 10:00pm (EDT), 4:00pm (CEST) Light field microscopy was first introduced in 2006, and allows users to capture the 4D light field within the microsco...
OCT 13, 2022 9:00 AM +08
OCT 13, 2022 9:00 AM +08
First Broadcast: Date: October 12, 2022 Time: 8:00am PDT, 11:00am EDT Second Broadcast: Date: October 12, 2022 Time: 9:00am SGT The new Embedded CryoSPARC Live, now fully integrated with t...
Loading Comments...
Show Resources
Attendees