Copy number variation, a major cause of structural variation in the genome, plays an important role in human disease. Determination of copy number variation requires both precision and accuracy. Real-time PCR is commonly used to validate copy number variation in populations. However, it may require many replicates to get the precision needed. Digital PCR allows you to go beyond the limits of real-time PCR. With the QuantStudio 3D Digital PCR System, a simple and affordable digital PCR platform, obtaining precise and accurate absolute quantification for your nucleic acid targets is attainable.