JAN 21, 2015 10:00 PM PST
NGS-Quality Sample Preparation with Covaris Adaptive Focused Acoustics (AFA™)
SPONSORED BY: Covaris, Inc.
8 42 4745

Speakers:
  • Principal Scientist, Covaris Inc.
    Biography
      Dr. Hamid Khoja brings years of experience in assay, protocol, and kit development in the field of molecular biology. Recently, Dr. Khoja has helped advance FFPE DNA and RNA extraction by utilizing Covaris' patented Adaptive Focused Acoustics Technology to develop a robust protocol and the truXTRAC™ purification kits. Previously, as a research scientist, Dr. Khoja developed several kits and high throughput assays for internal research initiatives, including branched DNA assays for oncogene quantification, Mass spectrometer based assays for CFTR and other disease mutation analysis, kits for ChIP-ChIP assays and the truChIP™ Chromatin Shearing reagent kits optimizing chromatin shearing for ChIP applications.

    Abstract:

     

    In this presentation we will illustrate the advantages of AFA technology in clinically significant applications (NGS, FFPE, ChIP) and will demonstrate the flexibility of Covaris AFA in delivering precise and highly-reproducible DNA and RNA fragmentation regardless of fragment size requirements


    NGS-quality DNA and RNA shearing

    Covaris AFA is considered to be the gold standard for DNA shearing for all NGS platforms in both R&D and clinical settings. Rapid advancements in genomic analysis technologies fuel the demand for advances in nucleic acid fragmentation technologies. In this presentation, we will discuss how Covaris AFA provides advanced sample preparation that out-performs the inherent limitations of ordinary sample prep technologies.

     

     NGS-quality FFPE nucleic acid extraction

    The novel truXTRAC™ method utilizes highly controlled focused acoustic energy for the effective removal of paraffin from FFPE cores, sections, and slides. truXTRAC enables rapid tissue rehydration, tissue digestion, crosslink reversal, and efficient nucleic acid release without the use of any dangerous organic solvents or messy mineral oils.  We will present data from a variety of tissue types and will compare the performance of different extraction methods on the same FFPE tissue section for the simultaneous extraction of NGS-quality DNA and total RNA. More importantly, for clinical medicine, we will also illustrate how the Covaris method translates into extraction and purification of high quality DNA and RNA for NGS library preparation


    Chromatin shearing for Chromatin Immunoprecipitation (ChIP)

    We will present data illustrating the use of isothermal and highly-controllable Covaris AFA technology for ChIP while maintaining a high degree of epitope integrity, and the universal AFA-optimized truChIP™ reagents developed to deliver exceptional fragmentation of chromatin from any mammalian cells or tissues.


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