AUG 20, 2014 08:00 AM PDT

High-resolution genomic analysis reveals genetic impacts of human papillomavirus in human cancers

C.E. CREDITS: CE | CEU
Speakers
  • Assistant Professor Department of Molecular Virology, Immunology and Medical Genetics, Assistant Professor, Division of Hematology, Department of Internal Medicine, Human Cancer Genetics Prog
    Biography
      Dr. David Symer is an assistant professor in the Human Cancer Genetics Program, Department of Molecular Virology, Immunology and Medical Genetics and Department of Internal Medicine, Division of Hematology at the Ohio State University Comprehensive Cancer Center - James Cancer Hospital. Recently, Dr. Symer also was named interim director of the core laboratory offering shared resources in genomics at Ohio State, the Nucleic Acids / Microarray Shared Resource. Dr. Symer has ongoing research interests in molecular biology and in cancer genetics and genomics. His primary clinical interest is chronic lymphocytic leukemia. He graduated with an AB degree in mathematics from Dartmouth College, earned his MD and PhD degrees at Johns Hopkins University School of Medicine, did his residency at Brigham and Women's Hospital (Harvard Medical School), and completed his fellowship training at Brigham and Women's Hospital and Johns Hopkins Oncology Center. Dr. Symer was a principal investigator at the National Cancer Institute prior to moving to Ohio State in 2009.

    Abstract:

    One of the hallmarks of human cancers is genetic instability. My colleagues and I recently identified a remarkable association between human papillomavirus (HPV) and genomic structural variation in flanking host genomic DNA, both in human cancer cell lines and primary tumors. A combination of whole-genome sequencing and other molecular assays revealed HPV integrants frequently adjacent to and bridging extensively amplified and rearranged genomic sequences, including deletions, inversions, and chromosomal translocations. We developed a model of looping to explain these data. Our model suggests that HPV integrant-mediated DNA replication and recombination may result in viralhost DNA concatemers, frequently disrupting genes involved in oncogenesis and amplifying the HPV oncogenes E6 and E7. Using innovative new long-range deep sequencing methods, we currently are characterizing in detail these genomic structural variants, induced by HPV integration in primary human cancers. Use of such high-resolution genomics methods has shed new light on a catastrophic process, distinct from chromothripsis and other mutational processes, by which HPV can induce genomic instability and disrupt candidate cancer-causing genes.


    Show Resources
    You May Also Like
    MAY 24, 2018 09:30 AM PDT
    C.E. CREDITS
    MAY 24, 2018 09:30 AM PDT
    DATE: May 24, 2018 TIME: 9:30PM PDT The current gold standard in in vitro pre-clinical cancer treatment screening remain cell lines,...
    MAY 03, 2018 11:00 AM PDT
    MAY 03, 2018 11:00 AM PDT
    DATE: May 3, 2018TIME: 11:00AM PDT, 2:00PM EDTWhile stress is one of the leading causes of neuropsychiatric disorders, the molecular underpinnings of how stress induces alterations in b...
    MAY 22, 2018 08:00 AM PDT
    C.E. CREDITS
    MAY 22, 2018 08:00 AM PDT
    DATE: May 22, 2018TIME: 08:00AM PDT The nuclear receptors pregnane X receptor (PXR) and constitutive androstane receptor (CAR) are closely related transcription factors that...
    APR 27, 2018 10:00 AM PDT
    C.E. CREDITS
    APR 27, 2018 10:00 AM PDT
    DATE: April 27, 2018TIME: 10:00am PST, 1:00pm ESTGlioblastoma (GBM) and Medulloblastoma (MB) are the most common adult and paediatric brain tumours, both of which can have devastating c...
    AUG 15, 2018 08:00 AM PDT
    C.E. CREDITS
    AUG 15, 2018 08:00 AM PDT
    DATE: August 15, 2018TIME: 08:00AM PDT, 11:00AM EDTThe failure of current chemotherapeutic strategies in the fight against cancer can be largely attributed to the occurrence of drug res...
    JUN 29, 2018 09:00 AM PDT
    C.E. CREDITS
    JUN 29, 2018 09:00 AM PDT
    DATE: June 29, 2018TIME: 09:00AM PDT, 12:00PM EDT There is significant epidemiological evidence to suggest that the consumption of a high-broccoli diet is associated with a r...
    Loading Comments...