JAN 27, 2017 4:00 AM PST

WEBINAR: Highly Multiplexed Single Cell Analysis Of Tumor Heterogeneity Through Time and Space by Mass Cytometry

Speakers

Abstract

DATE: January 27, 2017
TIME: 4:00am PT, 7:00am ET, 1:00pm CET

The study of the tumor ecosystem and its cell-to-cell communications is essential to enable an understanding of tumor biology, to define new biomarkers to improve patient care, and ultimately to identify new therapeutic routes and targets.

To study and understand the workings of the tumor ecosystem (TME), highly multiplexed image information of tumor tissues is essential. Such multiplexed images will reveal which cell types are present in a tumor, their functional state, and which cell-cell interactions are present. To enable multiplexed tissue imaging, we developed imaging mass cytometry (IMC). IMC is a novel imaging modality that uses metal isotopes of defined mass as reporters on antibodies and currently allows the visualization of over 50 proteins simultaneously on tissues with subcellular resolution. In the near future, we expect to be able to visualize over 100 proteins. Thus highly specific, reproducible and deeply validated antibodies are essential for IMC and any multiplexed antibody based method.
 
We applied IMC for the analysis of hundreds of breast cancer samples in a quantitative manner. Our analysis with a novel computational pipeline reveals a surprising level of inter and intra-tumor heterogeneity and identified new diversity within known human breast cancer subtypes, as well as a variety of stromal cell types that interact with them. Furthermore, we identified cell-cell interaction motifs in the tumor microenvironment correlating with clinical outcomes of the analyzed patients.
 
In summary, our results show that IMC provides targeted, high-dimensional analysis of cell type, cell state and cell-to-cell interactions within the TME at subcellular resolution. Spatial relationships of complex cell states of cellular assemblies can be used as biomarkers. We envision that IMC will enable a systems biology approach to understand and diagnose disease and to guide treatment.

Learning Objectives:

  • Learn about the novel imaging technique imaging mass cytometry (IMC)
  • Understand how antibody quality impacts highly multiplexed techniques

Show Resources
You May Also Like
MAY 11, 2021 10:00 AM PDT
C.E. CREDITS
MAY 11, 2021 10:00 AM PDT
Date: May 11, 2021 Time: 10:00zm PDT Your samples are some of the most valuable assets in the laboratory. After spending countless hours on extraction and preparation, your conclusions could...
JUN 09, 2021 7:00 AM PDT
C.E. CREDITS
JUN 09, 2021 7:00 AM PDT
Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...
APR 01, 2021 8:00 AM PDT
C.E. CREDITS
APR 01, 2021 8:00 AM PDT
Date: April 01, 2021 Time: 8:00am (PST), 11:00am (EST) Generating therapeutic antibodies is far more challenging than obtaining antibodies that merely recognize their targets. Engineering po...
JUL 15, 2021 9:00 AM PDT
Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
JUL 15, 2021 9:00 AM PDT
Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
Date: July 15, 2021 Time: 9:00am (PDT), 12:00pm (EDT) The Pisces workflow robust, easy-to-use, end-to-end multi-omics solution for highly multiplexed targeted Spatial RNA analysis. VeranomeB...
MAR 16, 2021 10:00 AM PDT
C.E. CREDITS
MAR 16, 2021 10:00 AM PDT
Date: March 16, 2021 Time: 10:00am (PST) Scientific progress and breakthroughs today are often too expensive for most institutions to acquire. Each year, the National Institutes of Health (N...
JUN 24, 2021 10:00 AM PDT
Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
C.E. CREDITS
JUN 24, 2021 10:00 AM PDT
Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
Date: June 24, 2021 Time: 10:00am PDT The Chan Zuckerberg Initiative (CZI) was founded to help solve some of society’s toughest challenges— from eradicating disease and improving...
Loading Comments...
Show Resources