OCT 28, 2014 8:00 AM PDT

The identification of single nucleotide and structural variants in gene sequencing

Speaker
  • Max van Min, CEO, CergentisLaurel Provencher, Manager of Assay Development, PerkinElmer
    Biography
      Max van Min is CEO of Cergentis and co-inventor of its Targeted Locus Amplification (TLA) Technology. Max received a Master of Science degree in Biotechnology from Wageningen University & Research Centre in the Netherlands and has a career in commercial management positions in the Life Sciences.
       
      Laurel is a Manager of Assay Development in the Microfluidics and Automation business unit of the Life Sciences and Technology division of PerkinElmer, Inc.  In the past ten years, Laurel has led various assay development projects including standardized automated NGS sample prep workflows, microfluidic analysis of DNA and protein samples, and microfluidic mobility-shift assays for drug discovery.  Her experience has included numerous collaborative projects in genomics, biotherapeutics and  small molecule drug development projects.

    Abstract
    The TLA Technology constitutes a paradigm shift in targeted next generation sequencing (NGS). The TLA technology uses the physical proximity of nucleotides within a locus of interest as the basis of selection. DNA is cross-linked, fragmented and ligated. Only one to a few primer pairs specific for a genetic locus of interest are in-turn required for the amplification of an entire locus. TLA  enrichment in combination with NGS is a very flexible and easy approach to sequence regions of interest and identify all single nucleotide variants (SNV's) and structural variants including comprehensive gene-fusion sequencing and the characterization of transgenes and their integration sites. Implementation of automated sample prep and QC for TLA technology will be discussed with an overview of liquid handling and microfluidic solutions for increasing productivity in NGS sample prep workflows.

    Show Resources
    You May Also Like
    JAN 23, 2020 9:00 AM PST
    C.E. CREDITS
    JAN 23, 2020 9:00 AM PST
    DATE: January 23, 2020 TIME: 9:00am PST, 12:00pm EST...
    APR 07, 2020 8:00 AM PDT
    C.E. CREDITS
    APR 07, 2020 8:00 AM PDT
    DATE: April 7, 2020 TIME: 8:00am PT, 11:00am ET This webinar sets out to establish why quality control is key to robust, reliable, reproducible science. We will look at best practice criteri...
    MAY 08, 2020 10:00 AM PDT
    C.E. CREDITS
    MAY 08, 2020 10:00 AM PDT
    DATE: May 8, 2020 TIME: 10:00am PT, 11:00am MT, 1:00pm ET The application of next generation sequencing to interrogate immune repertoires and methods in which these highly complex dataset...
    APR 27, 2020 9:00 AM PDT
    C.E. CREDITS
    APR 27, 2020 9:00 AM PDT
    DATE: April 27, 2020 TIME: 9:00am PT, 12:00pm ET Recombinant adeno-associated virus (rAAV) is a small (25-nm) virus that is composed of a protein shell called a capsid and a single-stranded...
    APR 29, 2020 8:00 AM PDT
    C.E. CREDITS
    APR 29, 2020 8:00 AM PDT
    Date: April 29, 2020 Time: 8:00AM PDT, 11:00AM EDT Single cell genomics and other next generation sequencing applications depend strongly on adequate upstream sample preparation. Results can...
    MAY 04, 2020 9:00 AM PDT
    C.E. CREDITS
    MAY 04, 2020 9:00 AM PDT
    DATE: May 4, 2020 TIME: 9:00am PT, 12:00pm ET...
    Loading Comments...
    Show Resources
    Attendees
    • See more