JUL 22, 2021 3:30 PM EDT

Improved chemistry by combining enzyme engineering, enzyme immobilization, and flow chemistry

C.E. Credits: P.A.C.E. CE Florida CE
Speaker

Abstract

Biocatalysis, protein engineering, and flow chemistry are key enabling technologies that can yield drastically shorter and greener chemical processes. We sought to leverage these technologies to realize an improved synthesis. A cheap and green chemical commodity was identified as a starting material that allowed the generation of a key intermediate in the synthesis in a stepwise reaction. As an initial proof of concept, an enzyme-catalyzed reaction was demonstrated with modest yields and selectivity. However, the initial enzyme and process would not be sufficient to support large scale synthesis. To improve the synthesis, we engaged in enzyme evolution, enzyme immobilization, and flow chemistry. Through several rounds of evolution the enzyme was evolved to reach high conversion, selectivity and stability in the presence of organic solvents.

Learning Objectives:

1. Know protein engineering is often necessary to improve desired properties of an enzyme to be used in commercial manufacturing routes.

2. Recognize enzyme immobilization along with protein engineering can be effectively used to drive high product conversion in organic solvents without loosing enzymatic activity and without the need of enzymatic removal during reaction work up.  


Show Resources
You May Also Like
FEB 24, 2021 10:00 AM PST
C.E. CREDITS
FEB 24, 2021 10:00 AM PST
DATE: February 24, 2021 TIME: 10am PST Automated lab instruments such as liquid handlers and cell sorters are increasingly common in all types of laboratories, driving fast results for labor...
SEP 14, 2021 7:00 AM PDT
C.E. CREDITS
SEP 14, 2021 7:00 AM PDT
Date: September 14, 2021 Time: 7am PDT, 10am EDT, 4pm CEST A conventional thermal cycler has long been a commodity product in the lab and end-point PCR techniques can be completed almost wit...
MAR 30, 2021 8:00 AM PDT
MAR 30, 2021 8:00 AM PDT
DATE: March 30, 2021 TIME: 08:00am PST In this talk, we will discuss the value of moving towards modular and automated, closed-system technologies designed to enable scalable and cost-effect...
APR 21, 2021 10:00 AM PDT
C.E. CREDITS
APR 21, 2021 10:00 AM PDT
Date: April 21, 2021 Time: 10:00am (PDT), 1:00pm (EDT) Serological testing for SARS-CoV-2 has been steadily adopted into clinical practice over the course of this pandemic. In this webinar,...
APR 07, 2021 9:00 AM PDT
C.E. CREDITS
APR 07, 2021 9:00 AM PDT
One challenge pharmaceutical companies face, is moving promising biotherapeutic products into the clinic as fast as safely possible. Devoting the correct amount of time and resources to earl...
C.E. CREDITS
Parkinson’s disease (PD) is the second most common neurodegenerative disorder with multiple motor and non-motor symptoms. PD is characterized by the presence on proteinaceous neuronal...
Loading Comments...
Show Resources