MAY 17, 2017 10:30 AM PDT

WEBINAR: Improved differentiation of stem cell-derived neurons and downstream applications through the reduction of progenitor proliferation with CultureOne™ Supplement

Speaker

Abstract

DATE: May 17, 2017
TIME: 10:30am PT, 1:30pm ET

Neurons derived from human pluripotent stem cells (hPSCs) and primary rodent neurons both are excellent resources for disease modeling and drug screening.  Human PSCs derived neural stem cells (NSCs) can be expanded in culture and further differentiated into mature neurons for various applications, however, these often contain mixed population of both differentiated neurons and undifferentiated NSCs. Due to the continuing proliferation of undifferentiated NSCs, very high cell densities and cell aggregation are usually observed during the differentiation of hPSC-derived NSCs which increase over time, posing challenges for long-term maintenance and downstream analysis.  Primary rodent neuronal cultures, while highly physiologically relevant, are often challenged by glial cell overgrowth, which may exacerbate assay and analysis issues.  Here we demonstrate the use of CultureOne(tm) - a new supplement which can reduce the proliferation of undifferentiated NSCs without negatively impacting the rate or extent of differentiation for hPSC-derived NSCs.  Further, we will demonstrate the ability to "tune" the glial cell population in primary rodent neuronal cultures.  The overall effect in both instances increases the relevant population of neurons in culture.  Experimental data presented in this webinar will  illustrate the functionality, morphology, and maturity of these neuronal cultures.


Show Resources
You May Also Like
MAY 11, 2021 10:00 AM PDT
C.E. CREDITS
MAY 11, 2021 10:00 AM PDT
Date: May 11, 2021 Time: 10:00zm PDT Your samples are some of the most valuable assets in the laboratory. After spending countless hours on extraction and preparation, your conclusions could...
JUN 09, 2021 7:00 AM PDT
C.E. CREDITS
JUN 09, 2021 7:00 AM PDT
Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...
SEP 14, 2021 7:00 AM PDT
C.E. CREDITS
SEP 14, 2021 7:00 AM PDT
Date: September 14, 2021 Time: 7am PDT, 10am EDT, 4pm CEST A conventional thermal cycler has long been a commodity product in the lab and end-point PCR techniques can be completed almost wit...
JUL 15, 2021 9:00 AM PDT
JUL 15, 2021 9:00 AM PDT
Date: July 15, 2021 Time: 9:00am (PDT), 12:00pm (EDT) The Pisces workflow robust, easy-to-use, end-to-end multi-omics solution for highly multiplexed targeted Spatial RNA analysis. VeranomeB...
APR 21, 2021 5:00 PM CEST
APR 21, 2021 5:00 PM CEST
Date: April 21, 2021 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) Spatial Answers Trilogy - Spatial Answers in Immunology Immunology Researchers share their Spatial Discoveries in SARS-C...
NOV 17, 2021 8:00 AM PST
C.E. CREDITS
NOV 17, 2021 8:00 AM PST
Date: November 17, 2021 Time: 8:00am (PDT), 11:00am (EDT) From waste disposal to promising biomarkers and therapeutic agents, exosomes and other extracellular vesicles are valuable in resear...
MAY 17, 2017 10:30 AM PDT

WEBINAR: Improved differentiation of stem cell-derived neurons and downstream applications through the reduction of progenitor proliferation with CultureOne™ Supplement


Specialty

Cell Biology

Cell Culture

Molecular Biology

Biotechnology

Neuroscience

Cancer Research

Clinical Research

Gene Expression

Cell Signaling / Transduction

Drug Discovery

Biomarkers

Stem Cell Technologies

Genetics

Immunology

Biochemistry

Geography

North America65%

Europe21%

Asia9%

South America2%

Oceania1%

Africa1%

Registration Source

Website Visitors100%

Job Title

Student17%

Research Scientist16%

Scientist15%

Post Doc9%

Executive9%

Facility/Department Manager8%

Medical Doctor/Specialist7%

Educator/Faculty6%

Medical Laboratory Technician4%

Clinical Laboratory Scientist4%

Biologist3%

Lab Management2%

Marketing/Sales1%

Organization

Academic Institution16%

Biotech Company8%

Hospital6%

Life Science Company6%

Research Institute5%

Pharmaceutical Company4%

Medical Center3%

Consultant3%

Clinical Laboratory3%

Non-Profit Organization2%

Medical School2%

Contract Research Organization (Cro)1%

Government1%

Ambulatory Care1%

Other7%


Show Resources
Loading Comments...
Show Resources