MAY 22, 2019 8:00 AM PDT

Improving lipidomics performance -- a view through a local lens

Sponsored by: Agilent
C.E. Credits: P.A.C.E. CE Florida CE
Speaker
  • Associate Professor at Brigham and Women's Hospital
    Biography
      Bruce Kristal received a BS in Life Sciences from MIT in 1986 and his PhD (Virology) from Harvard University in 1991. He rose from post-doc to Research Assistant Professor at the University of Texas Health Science Center at San Antonio (1991-1996), then moved to Burke Medical Research Institute (1996) and the Departments of Biochemistry (1997) and Neuroscience (1998) at Weill Medical College of Cornell University, becoming an Associate Professor in Neuroscience in 2004. He joined Brigham and Women's Hospital's Department of Neurosurgery in April 2007 and the Department of Surgery at Harvard Medical School in 2008 and transitioned to the Division of Sleep and Circadian Disorders in the Department of Medicine in 2016. Dr. Kristal was the founding secretary (2004-2008) and member of the Board of Directors of the Metabolomics Society (2004-2011). His laboratory currently has active projects in developing blood tests that predict future disease risk for preventable disorders (e.g., diabetes, breast and colon, heart disease), and the development of computer-based approaches to better deliver personalized medical care. His current projects focus on lipidomics biomarkers related to sleep, circadian biology, adiposity, and cardiometabolic dysfunction and disease.

    Abstract

    DATE: May 22, 2019
    TIME: 8:00am PDT

    Omics analysis offers the potential to obtain deep insight into biological processes as well as a rich source of potential biomarkers. While the consumers of such information are often unaware of the complexity of these analyses, “individuals skilled in the art” are fully aware of the multiple different levels at which improvements have driven the field, and which mistakes can mislead investigators. Here, I’ll use work from our laboratory to provide a broad view of these levels as they have played out for us in lipidomics. The talk will range from design (profiling vs targeted -- unexpected information from a profiling study), to sample acquisition and quality (lipids do degrade, the matrix makes a difference, and altering sample collection means altering the sample), to the effects of concentration and pressure on extraction, to the effects of ionization setting (the wrong settings increases false identifications) and running buffers (increasing sensitivity and improving identifications). The talk will close looking forward at some of the problems currently being worked on in the field.

    Learning Objectives:

    • Understanding the of stages of lipidomics

    • Understanding the potential pitfalls and opportunities at these stages

     

    For Research Use Only. Not for use in diagnostic procedures.

     

    LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.


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