DATE: May 22, 2019
TIME: 8:00am PDT

Omics analysis offers the potential to obtain deep insight into biological processes as well as a rich source of potential biomarkers. While the consumers of such information are often unaware of the complexity of these analyses, “individuals skilled in the art” are fully aware of the multiple different levels at which improvements have driven the field, and which mistakes can mislead investigators. Here, I’ll use work from our laboratory to provide a broad view of these levels as they have played out for us in lipidomics. The talk will range from design (profiling vs targeted -- unexpected information from a profiling study), to sample acquisition and quality (lipids do degrade, the matrix makes a difference, and altering sample collection means altering the sample), to the effects of concentration and pressure on extraction, to the effects of ionization setting (the wrong settings increases false identifications) and running buffers (increasing sensitivity and improving identifications). The talk will close looking forward at some of the problems currently being worked on in the field.

Learning Objectives:

• Understanding the of stages of lipidomics

• Understanding the potential pitfalls and opportunities at these stages

For Research Use Only. Not for use in diagnostic procedures.

LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.