In the last years, progress has been made regarding the differentiation of human pluripotent stem cells into neural stem cells and astrocytes, growing into neurospheres and cerebral organoids. These models offer an exciting new range of opportunities to investigate developmental changes associated with zika virus (ZIKV) infection in human neural cells. Using immunocytochemistry and electron microscopy, we showed that ZIKV targets human brain cells, reducing their viability and growth as neurospheres and brain organoids. Combining transcriptomics and proteomics, we showed that ZIKV alters the molecular fingerprint of neural stem cells by activating responses to viral replication, DNA damage targets, cell cycle arrest, apoptosis, as well as the downregulation of neurogenic programs. Our recent results will be discussed during the presentation.