DATE: February 22, 2018
TIME: 09:00am PST, 12:00pm EST, 06:00pm CEST(Europe)
Colorectal cancer (CRC) develops during a multi-step process from small lesions of the intestinal epithelium. Genetic mutations in the canonical Wnt signalling pathway are considered to be the initial step of tumour formation. Using 3D organoid technology, tumour organoid cultures from CRC patients can be established. CRC organoids closely recapitulate key properties of the original tumour epithelium, including histological appearance, general gene and protein expression pattern and mutational load. Tumour organoids are amenable to radiation treatment, gene-drug association studies and high-throughput drug screens. Organoid cultures from adjacent healthy colon mucosa that retain the identity of the healthy intestinal epithelium in vitro can also be generated. Sequential introduction of the most commonly mutated CRC genes (APC, P53, KRAS and SMAD4) into healthy colon organoids using CRISPR/Cas9 genome editing technology allows for the modelling of tumorigenesis in vitro. Xenotransplantation of mutated organoids into mice recapitulates critical features of CRC progression and metastasis. In sum, (cancer) organoid technology can be used as experimental tool for basic research as well as diagnostic and therapeutic tool for (personalised) medicine.