MENU
AUG 30, 2016 8:00 AM PDT

iPSC Generation and Validation: Ensuring the best materials for downstream applications iPSC Generation and Validation: Ensuring the best materials for downstream applications

Speaker
  • R&D Scientist, Cell Biology, Thermo Fisher Scientific
    Biography
      Rene has been a Research and Development scientist for the last 8 years at Thermo Fisher Scientific where his expertise extends to gene delivery methods, iPSC reprogramming, and cellular/molecular characterizations of pluripotent stem cells. He helped develop the Gibco PSC Training Course and has authored many peer reviewed articles and book chapters on Pluripotent Stem Cell characterization. Prior to joining Thermo Fisher Scientific, he was involved in over 6 years of professional basic research in academia in the fields of enzymology, stem cell biochemistry and neurogenesis, molecular genomics, and kinase biochemistry.

    Abstract
    The reprogramming of somatic cells into induced Pluripotent Stem Cells (iPSC) has great potential of applications, not only in basic research, but also in drug screening and cell therapy. With advances in reprogramming technologies, use of diverse media systems and somatic cell sources, there is a compelling need for comprehensive characterizations methods that facilitate the generation of high quality standardized iPSC cell banks

    The combined approach described in this presentation utilizes cellular and molecular techniques for qualifying the putative iPSC clones from the master somatic cell reprogramming population and through the subsequent maturation process. The resulting iPSC clones are further vetted for functional pluripotency, via expression of markers of self renewal and the pottential to differentiate into the 3 germ layers.  The resulting validated cell banks can help ensure  high quality of the cells prior to use in downstream applications such as gene editing and directed differentiation to specific lineages. A streamlined method of iPSC qualification and banking is critical for traceability to ensure the identity and quality of cells chosen for drug screening and cell therapy applications.

    Show Resources
    You May Also Like
    DEC 02, 2020 8:00 AM PST
    C.E. CREDITS
    DEC 02, 2020 8:00 AM PST
    DATE: December 2nd, 2020 TIME: 08:00am PDT, 11:00pm EDT Bioreactors and shakers are used to cultivate microorganisms, plant, insect, and mammalian cells in different volumes. Upscaling of pr...
    NOV 10, 2020 7:00 AM PST
    C.E. CREDITS
    NOV 10, 2020 7:00 AM PST
    DATE: November 10, 2020 TIME: 7:00am PDT, 10:00am EDT Automation can provide tremendous benefits such as increased pipetting precision and accuracy, productivity, and throughput. Numerous wo...
    NOV 16, 2020 8:00 AM PST
    C.E. CREDITS
    NOV 16, 2020 8:00 AM PST
    Date: November 16, 2020 Time: 8:00am (PST), 11:00am (EST) CRISPR screening has become the prime discovery tool in modern biomedical research and drug discovery. At the same time, most screen...
    AUG 18, 2020 10:00 AM PDT
    C.E. CREDITS
    AUG 18, 2020 10:00 AM PDT
    DATE: August 18, 2020 TIME: 10:00am PT Get deeper understanding of gene expression patterns by using assays that retain spatial organization at single cell resolution! Come learn about the n...
    SEP 10, 2020 9:00 AM PDT
    C.E. CREDITS
    SEP 10, 2020 9:00 AM PDT
    Date: September 10, 2020 Time: 9:00am (PDT), 12:00pm (EDT) Osmolality testing is relevant throughout the entire bioprocessing workflow. As customers look to refine mAb and gene therapy workf...
    OCT 08, 2020 7:00 AM PDT
    C.E. CREDITS
    OCT 08, 2020 7:00 AM PDT
    DATE: October 8, 2020 TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop thinking about ho...
    Loading Comments...
    Show Resources
    Attendees
    • See more