DATE: June 28, 2018
TIME: 11:00am EST/ 5:00pm CET
Enrichment of leukocytes from blood products like buffy coat, leukocyte reduction chambers (LRSC) or leukapheresis material can be time consuming and could influence the physiology of the target cells. Our new StraightFrom cell separation reagents were specifically developed to minimize time and handling steps, omitting the need to perform density gradient centrifugation methods or erythrocyte lysis prior to isolation thereby preserving cell integrity. Based on positive isolation, Th cells (CD4), cytotoxic T cells (CD8), Monocytes (CD14), B cells (CD19) or NK cells (CD56) can be isolated from complete buffy coats, LRSC or half pack LPs within less than 30 minutes with purities above 95%. The positive enrichment process enables thereby the consecutive enrichment of more than one cell population from a sample and is automatable. Using various assays, we show that functionality and viability of the target cells are preserved without cell activation. Ultimately, this optimized magnetic cells separation technology allows the isolation of physiologically relevant cells in a quick and easy manner for subsequent functional assay.
Isolated target cells have preserved functionality and viability