JUN 28, 2018 8:00 AM EDT

Isolation of immune cells Straight From whole blood, buffy coat, LRSC, and Leukopak without density gradients

Sponsored by: Miltenyi Biotec
Speakers

Abstract

DATE: June 28, 2018
TIME: 11:00am EDT/ 5:00pm CEST

Enrichment of leukocytes from blood products like buffy coat, leukocyte reduction chambers (LRSC) or leukapheresis material can be time consuming and could influence the physiology of the target cells. Our new StraightFrom cell separation reagents were specifically developed to minimize time and handling steps, omitting the need to perform density gradient centrifugation methods or erythrocyte lysis prior to isolation thereby preserving cell integrity. Based on positive isolation, Th cells (CD4), cytotoxic T cells (CD8), Monocytes (CD14), B cells (CD19) or NK cells (CD56) can be isolated from complete buffy coats, LRSC or half pack LPs within less than 30 minutes with purities above 95%. The positive enrichment process enables thereby the consecutive enrichment of more than one cell population from a sample and is automatable. Using various assays, we show that functionality and viability of the target cells are preserved without cell activation. Ultimately, this optimized magnetic cells separation technology allows the isolation of physiologically relevant cells in a quick and easy manner for subsequent functional assay.

Learning Objective:

  • The StraightFrom MicroBeads enables magnetic cell separation without density gradient centrifugation
  • Get immune cells faster and easier from blood  and blood products
  • Isolated target cells have preserved functionality and viability


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JUN 28, 2018 8:00 AM EDT

Isolation of immune cells Straight From whole blood, buffy coat, LRSC, and Leukopak without density gradients

Sponsored by: Miltenyi Biotec

Specialty

Immunology

Cancer Research

Cell Biology

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