MAY 20, 2020 7:30 AM PDT

Keynote Presentation: Bridging Multiscale Physiological Systems via Microfluidics- from single cell to tissue-scale to organ-scale

C.E. Credits: P.A.C.E. CE Florida CE
Speaker
  • Professor, Biomedical Engineering, University of California, Irvine Director, NSF I/UCRC Center for Advanced Design & Manufacturing of Integrated Microfluidics (CADMIM), Lab On A Chip Journal
    Biography
      Abraham (Abe) P. Lee is Professor of Biomedical Engineering (BME) and Mechanical and Aerospace Engineering (MAE) at the University of California, Irvine. He is Director of the NSF I/UCRC "Center for Advanced Design & Manufacturing of Integrated Microfluidics" (CADMIM). Currently Dr. Lee serves as Editor-in-Chief for the Lab on a Chip journal. Prior to UCI, he was a program manager in the Microsystems Technology Office at DARPA (1999-2001), Senior Technology Advisor at National Cancer Institute (NCI) and a group leader with Lawrence Livermore National Lab (LLNL). Over the years, Dr. Lee has pioneered research in applying microfluidics to biomedical applications, and currently focuses on integrated microfluidic systems for precision medicine. His research has contributed to the founding of several start-up companies. He owns 45 issued US patents and is author of over 100 journals articles. Professor Lee was awarded the 2009 Pioneers of Miniaturization Prize and is an elected fellow of the National Academy of Inventors (NAI), the American Institute of Medical and Biological Engineering (AIMBE), the Royal Society of Chemistry (RSC), the American Society of Mechanical Engineering (ASME), and the Biomedical Engineering Society (BMES).

    Abstract

    Precision medicine is the paradigm to develop treatments for patients based on molecular-targets that are effective in vivo when administered.  In addition to identifying the molecular and cellular targets that are the source of disease, it is also critical to understand how these targets behave in the body based on physiological principles.  Recent developments in microfluidics have contributed to burgeoning precision medicine fields such as liquid biopsy, immunotherapy, single cell analysis, genotyping and gene sequencing, and microphysiological systems.  The fact that microfluidics bridges the scales of molecular, cellular, tissue, and can even recapitulate organ and circulatory functions of the body enables a microprocessor for a plethora of health indicators. In liquid biopsy, microfluidics can analyze biological samples such as blood for the detection of biomolecules or cells that are indicative of disease or physiological state. Our microfluidic platform, the lateral cavity acoustic transducers (LCAT) can process a drop of blood to 1) sort undiluted donor whole blood into its cellular subsets (plasma, RBCs, and WBCs), 2) enrich and retrieve spiked breast cancer cells at rare cell relevant concentrations (10 per mL), and 3) on-chip immunofluorescent label specific target cellular populations by their known marker expression patterns.  The ultimate goal is to develop a comprehensive panel that can provide a holistic representation of the body’s health status, including molecular and cellular readout that indicate infections, immune status, metabolic status, gene expressions, and cytokine levels.  Furthermore, recent developments in cell therapies requires new approaches towards engineering cells without the complications and limitations of viral vectors. I will present a five-step protein array assay in which HIV, HPV and HSV reactive antibodies from both serum and saliva were rapidly detected by an integrated, rapid and simple-to-use multiplexed microfluidic device. Another example I will present is a 3-D vascularized micro organ (VMO) system connects microfluidic channels to vascularized tissues, forming the basis of the “human body on-chip”.  Applications for this platform include microphysiological systems for screening of drugs, studying vascular malformations, and understanding immune responses. Ultimately this on-chip microcirculation platform maintains ‘microfluidic homeostasis’ for studying bottom up treatment options.

    Learning Objectives:

    1. Identify the potential for microfluidic devices for multi-scale processing of blood components

    2. Describe the approaches for microfluidics to tap into the current cellular and immuno- therapies

    3. Explain the importance of vasculature for microphysiological systems


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