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Nov 12, 2020

Principles of Effective Method Development in Pathobiology Research

C.E. Credits: P.A.C.E. CE Florida CE
Speaker
  • Gilead Sciences, Foster City, CA Jan., 2011 - current Associate Scientist I (2011-2013), Biologics and Target Biology Associate Scientist II (2014-2015), Biomarker / Biology Core Support
    Biography

      Vivian Barry is a Senior Scientist in the Pathobiology group at Gilead Sciences.  She began her career in molecular biology and immunology research areas at DNAX and Genentech.  She eventually ventured into histopathology research and became the manager of the immunohistochemistry team at Arresto Biosciences in 2008.  Vivian joined Gilead in 2011, with the acquisition of Arresto, and has been managing the IHC/Pathology group at Gilead since that time.  Over the years, she has focused on building the histopathology lab and has introduced various machinery and techniques to enhance the delivery of high quality IHC and CISH data.  Her team focuses on target validation in both human tissues and animal models, assists in determining mechanism of action of therapeutics and small molecules, and is involved in IND-enabling studies.  She collaborates with investigators across Gilead's departments; these projects include virology, immunology, oncology, and fibrotic diseases.  Vivian received her Bachelor of Science and Master of Science degrees at the University of San Francisco, and has recently received her MBA from the Golden Gate University, San Francisco.


    Abstract

    Immunohistochemistry (IHC) is used to determine spatial relationships where we can identify the localization of target proteins in specific regions and cells of a tissue.  IHC has been the gold standard to analyze protein expression analysis in tissues; however, the IHC assay may not always work as not all antibodies are robust reagents for detection of target proteins.  For that reason, we have utilized several orthogonal assays to analyze localization of proteins and mRNA of targets within cells where robust antibody reagents are not available or specificity is in question. I will briefly describe several effective techniques that have helped my pathobiology team in the method development of assays that are used for target validation and characterization of human diseases and animal disease models.

    Learning Objectives:

    1. The presentation will provide the audience the many tools available for the method development of antibodies by utilizing orthogonal methods to ensure specificity of the IHC assay or equivalent histology-related assay

    2. The presentation will help you discern how to resolve common problems encountered in immunohistochemical staining


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