OCT 24, 2018 09:00 AM PDT

Keynote Presentation: Solid Phase Microextraction: New Developments in Bioanalysis and Medical Applications

C.E. CREDITS: P.A.C.E. CE | Florida CE
Speakers
  • University Professor, Canada Research Chair, Department of Chemistry, University of Waterloo
    Biography
      Dr. Barbara Bojko is postdoctoral fellow in Prof. Pawliszyn's group at Department of Chemistry, University of Waterloo (Canada) and assistant professor at Department of Medicinal Chemistry, Collegium Medicum Nicolaus Copernicus (Poland). Previously she was an assistant professor at Medical University of Silesia, Poland. In 2001 she obtained her medical diagnostician diploma and master degree, and in 2005 she obtained the PhD (Hon) in pharmaceutical sciences, with specialization in pharmaceutical biochemistry. She is an author of over 40 publications in peer-reviewed journals and two book chapters. She was awarded for her scientific achievements by Minister of Health (2004, 2007, 2011) and for her academic achievements by President of Medical University of Silesia (2009, 2010, 2012).
    • University Professor, Canada Research Chair, Department of Chemistry, University of Waterloo
      Biography
        Janusz B. Pawliszyn received his early education in Poland. He earned his B.Sc. in Engineering at the Technical University of Gdansk in 1977, his M.S. in organic chemistry at the same university in 1978, and his Ph.D. in analytical chemistry at Southern Illinois University in Carbondale in 1982. Then he spent two years as a Postdoctoral Fellow at the University of Toronto, Canada, and joined the faculty at Utah State University as an Assistant Professor. In 1988 he moved to The University of Waterloo, Waterloo, Ontario where he moved up the ranks to Associate Professor in 1991 and Professor in 1997. Professor Pawliszyn is an author of over 150 scientific publications and has recently published a book on Solid Phase Microextraction. He has graduated numerous M.S. and Ph.D. students as well as hosting many visiting scholars. He has served as a member of the Editorial Boards of Analytical Chemistry, Analyst, Canadian Journal of Chemistry, Electrophoresis, Journal of Microcolumn Separations, and Field Analytical Chemistry and Technology. He received the 1995 McBryde Medal, the 1996 Tswett Medal, the 1996 Hyphenated Techniques in Chromatography Award, the 1996 Caledon Award, the 1998 Jubilee Medal from the Chromatographic Society, U.K., and the 2000 Maxxam Award (CSC). Professor Pawliszyn has held Visiting Professorships to the University of Campinas, Campinas, Brazil, to the Centre for environmental Research, Leipzig, Germany, and to Toyohashi University of Technology sponsored by the Research Foundation for the Electrotechnology of Chubu. Professor Janusz B. Pawliszyn is the recipient of the Southern Illinois University College of Science Alumni Achievement Award for 2000.

      Abstract:

      Solid phase microextraction (SPME) is a versatile, non-exhaustive sample preparation tool that has been demonstrated to be well-suited for facile and effective analysis of a broad range of compounds in a plethora of studies. A growing number of reports describing diverse SPME workflows for novel investigations in a variety of fields including diverse bioanalytical applications, among others, corroborate the applicability of this microextraction tool in the bioanalytical sciences. Important bioanalytical applications of SPME include high throughput, in vivo and on-site analysis, in vitro and in vivo metabolomics studies, pharmaceutical and biomedical research. Patient monitoring, metabolomics and biomarkers discovery are an integral part of medical diagnosis and biomedical research. However, tissue analysis remains the bottleneck of such studies due to the invasiveness of presently used sample collection based on biopsy, as well as the laborious and time-consuming sample preparation protocols. In the proposed technology, we have integrated in vivo sampling, sample preparation including global extraction of metabolites together with convenient coupling to analytical instrument. This approach based on a small sorbent coated flexible metal fiber format of solid phase microextraction was developed and evaluated during organ treatment and transplantation in pig model as well as applied to human organs.  In this chemical biopsy approach, only molecules of interests are extracted onto biocompatible sorbent from the investigated system, however tissue is not removed from the organ, just metabolites of interest.  When using extraction phase with appropriate chemical property and morphology, the analytes are enriched onto the sorbent with minimum of interferences collected.  Therefore, the direct coupling of the chemical biopsy probe to mass spectrometry is feasible.  Such hyphenation facilitates rapid on-site quantification of the compounds of interests resulting in possibility of close to real time monitoring.  This approach has potential in biological and clinical on-site investigations including bedside medical diagnosis.

      Learning Objectives:

      1. Provide understanding about microextraction approaches and their advantages compared to exhaustive extraction techniques.
      2. To demonstrate potential of the SPME technology in biomedical research.


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