NOV 04, 2021 8:00 AM PDT

Label 3Differently - how 3D-IF-validated antibodies provide clear signals from cleared tissues in large-scale 3D imaging

Sponsored by: Miltenyi Biotec
Speaker

Abstract
Date:  November 4, 2021
Time: 8:00am (PDT),  11:00am (EDT)
 
The promise of tissue clearing techniques in combination with 3D-immunofluorescence (IF) microscopy is revolutionary. Complex biological systems could suddenly become available in detail, without the need for the tissue sectioning necessitated by today’s “gold standard” immunohistochemistry. You can analyze tumor-infiltrating immune cells, the architecture of rodent nervous systems, and much more, in full spatial detail without laborious processing of individual sections and the potential loss of critical information this inevitably entails. If only reliable tissue clearing and immunolabeling protocols, not to mention the “small” detail of validating antibodies for 3D-IF applications, wasn’t prohibitively time-consuming and costly. To support researchers in their endeavors, Miltenyi Biotec has developed solutions for the main steps of the cleared tissue imaging workflow.
 
In this talk, Markus Habich will give insight into the basics of tissue clearing and immunolabeling techniques as well as on the different challenges of tissue clearing and immunolabeling. Also, Markus will present how the use of 3D-IF-validated recombinant antibodies, easy-to-use tissue clearing reagents and protocols, and the newly developed UltraMicroscope Blaze™ Light Sheet System help to overcome these challenges.
 
 
 
 
Webinars will be available for unlimited on-demand viewing after live event.
 
 

Show Resources
You May Also Like
APR 26, 2022 7:00 AM PDT
C.E. CREDITS
APR 26, 2022 7:00 AM PDT
Date: April 19, 2022 Time: 7:00am (PDT), 10:00am (EDT), 4:00pm (CEST) High-content (HC) phenotypic profiling approaches are a powerful tool to study the effect of biological, genetic, and ch...
MAY 17, 2022 9:00 AM PDT
MAY 17, 2022 9:00 AM PDT
Date: May 17, 2022 Time: 9:00am (PDT), 12:00pm (EDT), 8:00pm (CEST) Gene therapeutics have great potential to treat many severe diseases in an unprecedented, targeted manner. The biopharmace...
JUN 21, 2022 6:00 AM PDT
JUN 21, 2022 6:00 AM PDT
Date: June 21, 2022 Time: 6:00am (PDT), 9:00am (EDT), 3:00pm (CEST) The global understanding and practice of medicine is currently undergoing a revolutionary change. This shift to precision...
APR 28, 2022 8:00 AM PDT
APR 28, 2022 8:00 AM PDT
Date: April 28, 2022 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) Human pluripotent stem cells (PSCs) and their derivatives hold great potentials in...
MAR 02, 2022 9:00 AM PST
C.E. CREDITS
MAR 02, 2022 9:00 AM PST
Date: March 02, 2022 Time: 9:00am (PST), 12:00pm (EST) Single cell RNA-seq is known to only capture a small fraction of the transcriptome of each cell. Often, this is due to inherent limitat...
MAR 16, 2022 8:00 AM PDT
C.E. CREDITS
MAR 16, 2022 8:00 AM PDT
Date: March 16, 2022 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CET) Handling of potent and/or hazardous substances is commonplace in sev.....
Loading Comments...
Show Resources