DATE: November 12, 2019
TIME: 10:00am PST
Direct measurement of copy number by droplet-based shallow sequencing of genomic DNA has the potential to provide new insights into tumor heterogeneity and tumor evolution. We examine its utility in a well-studied cancer cell line within a series of tumor specimens. We will demonstrate how clustering based on single cell copy number can help identify subclones that, in turn, make it possible to resolve point mutations, structural variants, and loss of heterozygosity, leading to a clearer picture of overall sample heterogeneity. We will present how we were able to gain significant novel insights—even in well-studied systems—by integrating these clone-specific variants and building models from single cell sequencing of DNA. Finally, we will discuss the design considerations, such as sequencing depth and number of cells, that were important in these studies.
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