AUG 20, 2015 08:00 AM PDT

WEBINAR: Light Sheet Microscopy for Tissue Clearing Applications & 3D Live Fluorescent Imaging

SPONSORED BY: Andor, Andor
  • Postdoctoral fellow, California Institute of Technology
      Alon holds his BS and MS degrees in Electrical Engineering from Tel Aviv University, and his Ph.D in Electrical Engineering from University of California, Los Angeles. In his doctorate degree, Alon conducted biophotonics research under Prof. Aydogan Ozcan, focusing on high-resolution and high-throughput microscopy for biomedical applications. In Prof. Viviana Gradinaru's lab (California Institute of Technology), he is exploring innovative imaging techniques for various neuroscience applications.
    • Assistant Research Fellow, Research Center for Applied Science, Academia Sinica, Taiwan
        Bi-Chang Chen received the B.S. and M.S. degrees from Chemistry Department of National Taiwan University, Taiwan in 2001 and 2003, separately. For the M.S. work, he worked on the mesoporous material synthesis under the supervision of Prof. Chung-Yuan Mou. After two-year military service, he moved to States in 2006 to complete his Ph.D. degree in chemistry at the University of Texas at Austin, where he studied about the Coherent anti-Stokes Raman Imaging technique in Dr. Sang-Hyun Lim group. After five-year training, he joined Dr. Eric Betzig's group at 2011 summer to continue his postdoctoral work to develop the cutting edge imaging tools about 3D live fluorescent microscopy, lattice light sheet microscopy. In 2014 spring, he became an Assistant Research Fellow at Research Center for Applied Science, Academia Sinica in Taiwan. He is interested in developing fast, low phototoxicity, multi-color, and 3D detection for fluorescent living specimens with subcellular resolution imaging tools.
      • Life Science Application Specialist, Andor Technology
          Dr. Hanrahan has worked with Andor Technology as an Application Specialist in Life Science for the past five years. Her role brings her in touch with all the latest developments and innovations in both camera technology and microscopy applications.
          Previous to this Dr. Hanrahan managed a microscopy facility in the Biochemistry Department at Trinity College, Dublin where she worked with a variety of microscopy set-ups including wide-field fluorescent microscopy, Point Scanning Confocal Microscopy, and Spinning Disk Confocal Microscopy. Her interest in microscopy stemmed from her Ph.D. in Biochemistry, during which she gained experience with numerous microscopy techniques used in her graduate research.

        DATE: August 20, 2015
        TIME: 8:00am PDT, 11:00am EDT, 4:00pm BST

        Light Sheet Microscopy for 3D live fluorescent imaging at high spatiotemporal resolution
        Dr Bi-Chang Chen will discuss his research in developing cutting edge imaging tools for 3D live fluorescent microscopy, lattice light sheet microscopy.

        This will include an in-depth look at how Light Sheet microscopy has been used to generate a series of 2D images on a camera, which can then be assembled into a 3D image. The process is then repeated to build a 4D data set of cellular dynamics.

        The technology collects high-resolution images rapidly and minimizes damage to cells, meaning it can image the three-dimensional activity of molecules, cells, and embryos in fine detail over longer periods than was previously possible.

        Light-Sheet Microscopy for Tissue Clearing Applications
        In the process of learning about complex organs such as the brain and the gut, it is essential to map their 3D structure with microscopic resolution, and ideally, study them as a whole in order to maintain their structural properties.

        In this talk, Dr Alon Greenbaum will present some of the unique properties that light-sheet microscopy has to offer for tissue clearing, as well as discuss the technical challenges encountered while building a custom made light-sheet microscope and their solutions.

        Zyla 4.2 sCMOS -The Ideal Detector for Light Sheet Microscopy
        Dr Orla Hanrahan from Andor Technology will discuss the latest developments in sCMOS technology with an in-depth look at the Zyla 4.2 - the ideal detector for light sheet microscopy.

        Offering the highest QE, extremely low read noise and fast frame rate, Zyla 4.2 is ideal for a wide range of applications. LightScan PLUS, a new feature on Zyla 4.2. benefits the researcher by increasing contrast and degree of confocality. Extremely high frame rates can be achieved and easy synchronization of the rolling shutter with the scanned illumination beam results in confocal line detection.

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