Light Sheet Microscopy for 3D live fluorescent imaging at high spatiotemporal resolution
Dr Bi-Chang Chen will discuss his research in developing cutting edge imaging tools for 3D live fluorescent microscopy, lattice light sheet microscopy.
This will include an in-depth look at how Light Sheet microscopy has been used to generate a series of 2D images on a camera, which can then be assembled into a 3D image. The process is then repeated to build a 4D data set of cellular dynamics.
The technology collects high-resolution images rapidly and minimizes damage to cells, meaning it can image the three-dimensional activity of molecules, cells, and embryos in fine detail over longer periods than was previously possible.
Light-Sheet Microscopy for Tissue Clearing Applications
In the process of learning about complex organs such as the brain and the gut, it is essential to map their 3D structure with microscopic resolution, and ideally, study them as a whole in order to maintain their structural properties.
In this talk, Dr Alon Greenbaum will present some of the unique properties that light-sheet microscopy has to offer for tissue clearing, as well as discuss the technical challenges encountered while building a custom made light-sheet microscope and their solutions.
Zyla 4.2 sCMOS -The Ideal Detector for Light Sheet Microscopy
Dr Orla Hanrahan from Andor Technology will discuss the latest developments in sCMOS technology with an in-depth look at the Zyla 4.2 - the ideal detector for light sheet microscopy.
Offering the highest QE, extremely low read noise and fast frame rate, Zyla 4.2 is ideal for a wide range of applications. LightScan PLUS, a new feature on Zyla 4.2. benefits the researcher by increasing contrast and degree of confocality. Extremely high frame rates can be achieved and easy synchronization of the rolling shutter with the scanned illumination beam results in confocal line detection.