Measuring Outcomes of CRISPR Experiments & Leveraging Cas9 for Targeted Sequencing

Presented at: CRISPR 2020
C.E. Credits: P.A.C.E. CE Florida CE
Speaker
  • Chief Scientific Officer, Pacific Biosciences
    Biography
      Jonas Korlach was appointed Chief Scientific Officer of Pacific Biosciences in July 2012. He was previously a Scientific Fellow, supporting commercial development of the PacBio RS II system and performing research aimed at developing new applications for SMRT technologies. He co-invented the SMRT technology with Stephen Turner, Ph.D., Pacific Biosciences Founder and Chief Technology Officer, when the two were graduate students at Cornell University.

      Dr. Korlach joined Pacific Biosciences as the company's eighth employee in 2004. Previously, he was a Postdoctoral Researcher at Cornell University. Dr. Korlach is the recipient of multiple grants, an inventor on 70 issued U.S. patents and 61 international patents, and an author of over 100 scientific studies on the principles and applications of SMRT technology, including publications in Nature, Science, and PNAS. In 2013, Dr. Korlach was honored by the Obama White House as an Immigrant Innovator "Champion of Change." He received both his Ph.D. and his M.S. degrees in Biochemistry, Molecular and Cell Biology from Cornell, and received M.S. and B.A. degrees in Biological Sciences from Humboldt University in Berlin, Germany.

    Abstract

    CRISPR experiments are a powerful tool which are easy to carry out, however it is more difficult to determine the outcome of these experiments, and to ensure that only the desired targets were addressed and were so correctly. I will describe new methods to evaluate the outcomes of CRISPR experiments and highlight their importance for characterizing the results and lay a foundation for future optimizations. In addition, I will describe new methods to leverage the specificity of Cas9 to target genomic regions without amplification for sequencing with high accuracy and multiplex capabilities.

    Learning Objectives:

    1. Understand the importance of measuring outcomes of CRISPR experiments, limitations of previous approaches, and the benefits of new methods

    2. Learn about new methods to leverage Cas9 to target genomic regions for sequencing without amplification with high specificity, accuracy and multiplex


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