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MAY 10, 2017 1:00 PM PDT

Modification of Glycans in Bioprocessing

Speaker
  • Scientist III, Cell Biology, Bioproduction Research & Development, Thermo Fisher Scientific
    Biography
      Ryan Boniface has been an R&D Scientist with Thermo Fisher Scientific in development of Gibco™ media and supplement products for nine years. He is experienced in high-throughput medium design process development, validation, and technology transfer. Ryan's formal education includes an MS in Biotechnology from State University of New York, Buffalo.

    Abstract

    Glycosylation is an important product quality attribute for biotherapeutic proteins expressed in CHO cells. Glycoform variability can significantly affect the safety and efficacy of therapeutic proteins, and can be dependent on several factors, including cell line, media/feeds, and process. As a consequence, it has often been challenging to achieve and maintain preferred glycosylation profiles from cell culture development through bioreactor scale-up. To address these challenges, we have developed a new feed technology in conjunction with a unique fed-batch process that together has been shown not only to maximize protein titers but also to modulate glycan profiles.


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    Modification of Glycans in Bioprocessing


    Specialty

    Biotechnology

    Microbiology

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    Molecular Biology

    Antibodies

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    Cancer Therapeutics

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