DEC 05, 2018 9:00 AM PST

Making the most of your mouse model with bead-based multiplexing

Sponsored by: Luminex
Speaker
  • Proteomics Field Application Scientist Leader, Bio-Rad Laboratories
    Biography
      Dr. Daniel Braunschweig received his PhD in Immunology from the University of California, Davis, studying the role of the immune system in autism. He subsequently published more than 15 peer-reviewed articles on the interface between neurodevelopment and immunology, with an emphasis on emerging immunoassay techniques. Dr. Braunschweig has held a variety of positions in the Biotechnology field, and currently leads a team of Field Application Scientists focused on assisting researchers with protein purification and immunoassays.

    Abstract

    DATE: December 5, 2018
    TIME:  9:00AM PST

    The mouse is a critical, increasingly used model system in biomedical research and preclinical drug discovery. While technological advances enable complex genetic manipulations that result in increased translational relevance, one distinct limitation remains - sample volume.

    In addition, preclinical research increasingly demands measurement of multiple biomarkers (cytokines, chemokines, and growth factors) to characterize signaling pathways accurately or to analyze the effects of an intervention. Unfortunately, researches are often forced to design experiments around sample limitations.

    The Bio-Plex® Multiplex System, powered by Luminex xMAP® Technology enables the quantification of more than 500 different protein or peptide targets simultaneously from a single sample volume. More specifically, the Bio-Plex Pro™ Mouse Cytokine, Chemokine, and Growth Factor Magnetic Bead-Based Assays detect and measure levels of 46 analytes in diverse matrices such as serum, plasma, and tissue culture supernatants.

    This presentation will provide an overview of multiplexing technology for research use with mouse samples, and highlight the associated benefits to the drug discovery workflow.

     

    Learning Objectives:

    • What xMAP Technology is and how it is used
    • Mouse models in research
    • Common challenges and solutions with multiplexing technology
    • Features of xMAP-based multiplexing vs. other options for quantitation:

              - Reduced sample volume usage

              - Enhanced dynamic range and quantitation in contrast to ELISA

              - Global profiling and pathway analysis

              - Increased throughput capabilities coupled with reduced cost and time inputs

              - Superior data accuracy


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