MAY 11, 2017 12:00 PM PDT

New multiplexed nCounter® PlexSet™ Reagents- an alternative to qPCR technology

Sponsored by: NanoString Technologies
Speaker
  • the Molecular Biology Core Facility Manager, Geisel School of Medicine at Dartmouth
    Biography
      Christian H. Lytle is the Molecular Biology Core Facility Manager at Geisel School of Medicine at Dartmouth. He has more than 30 years of research experience in various roles and is affiliated with societies such as The Association of Bimolecular Resource Facilities (ABRF). He has received many awards and recognitions for his work in the genomics field.

    Abstract

    The nCounter® Analysis System from NanoString® Technologies uses a novel molecular barcoding technology to measure multiplexed gene expression of up to 800 targets with simple, enzyme- free, direct probe hybridization and tabulates fluorescent barcodes to provide specific and precise digital data. The standard nCounter gene expression assay includes 12 samples per run. Here we present data from the newly released nCounter PlexSet™ assay which enables researchers to quickly evaluate up to 24 multiplexed targets directly from cell lysates for 96 samples per run. PlexSet Reagents are universal and can be combined with gene-specific probe oligos to digitally quantify any 24 targets directly from cell lysates and total purified RNA from FFPE samples.

     

    The direct digital quantification (no enzymes or amplification), lyse-and-go capability, technical assay controls, high sensitivity, and simple data analysis make this an ideal alternative to standard qRT-PCR. We will report on our experience using this technology, and why we believe it could easily replace many of the qRT-PCR assays being conducted.


    Show Resources
    You May Also Like
    DEC 02, 2020 8:00 AM PST
    C.E. CREDITS
    DEC 02, 2020 8:00 AM PST
    DATE: December 2nd, 2020 TIME: 08:00am PDT, 11:00pm EDT Bioreactors and shakers are used to cultivate microorganisms, plant, insect, and mammalian cells in different volumes. Upscaling of pr...
    OCT 08, 2020 7:00 AM PDT
    C.E. CREDITS
    OCT 08, 2020 7:00 AM PDT
    DATE: October 8, 2020 TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop thinking about ho...
    NOV 10, 2020 7:00 AM PST
    C.E. CREDITS
    NOV 10, 2020 7:00 AM PST
    DATE: November 10, 2020 TIME: 7:00am PDT, 10:00am EDT Automation can provide tremendous benefits such as increased pipetting precision and accuracy, productivity, and throughput. Numerous wo...
    NOV 16, 2020 8:00 AM PST
    C.E. CREDITS
    NOV 16, 2020 8:00 AM PST
    Date: November 16, 2020 Time: 8:00am (PST), 11:00am (EST) CRISPR screening has become the prime discovery tool in modern biomedical research and drug discovery. At the same time, most screen...
    OCT 29, 2020 6:00 AM PDT
    C.E. CREDITS
    OCT 29, 2020 6:00 AM PDT
    Date: October 29, 2020 Time: 6:00am (PDT), 9:00am (EDT), Chronic inflammation can occur as a result of a combination of genetic predispositions and environmental factors. Epigenetic modifica...
    DEC 16, 2020 8:00 AM PST
    C.E. CREDITS
    DEC 16, 2020 8:00 AM PST
    Date: December 16, 2020 Time: 8:00am (PST), 11:00am (EST) Molecular imaging of living specimens offers a means to draw upon the growing body of high-throughput molecular data to better under...
    MAY 11, 2017 12:00 PM PDT

    New multiplexed nCounter® PlexSet™ Reagents- an alternative to qPCR technology

    Sponsored by: NanoString Technologies

    No demographic data is available yet for this event.


    Show Resources
    Loading Comments...
    Show Resources