MAY 11, 2017 12:00 PM PDT

New multiplexed nCounter® PlexSet™ Reagents- an alternative to qPCR technology

Sponsored by: NanoString Technologies
Speaker

Abstract

The nCounter® Analysis System from NanoString® Technologies uses a novel molecular barcoding technology to measure multiplexed gene expression of up to 800 targets with simple, enzyme- free, direct probe hybridization and tabulates fluorescent barcodes to provide specific and precise digital data. The standard nCounter gene expression assay includes 12 samples per run. Here we present data from the newly released nCounter PlexSet™ assay which enables researchers to quickly evaluate up to 24 multiplexed targets directly from cell lysates for 96 samples per run. PlexSet Reagents are universal and can be combined with gene-specific probe oligos to digitally quantify any 24 targets directly from cell lysates and total purified RNA from FFPE samples.

 

The direct digital quantification (no enzymes or amplification), lyse-and-go capability, technical assay controls, high sensitivity, and simple data analysis make this an ideal alternative to standard qRT-PCR. We will report on our experience using this technology, and why we believe it could easily replace many of the qRT-PCR assays being conducted.


Show Resources
You May Also Like
JUN 28, 2022 7:00 AM PDT
JUN 28, 2022 7:00 AM PDT
Date: June 28, 2022 Time: 3:00pm (BST), 4:00pm (CET), 9:00am (CST), 7am (PST) Light-sheet microscopy is an extremely versatile imaging technique with a vast range of implementations that are...
MAY 17, 2022 9:00 AM PDT
MAY 17, 2022 9:00 AM PDT
Date: May 17, 2022 Time: 9:00am (PDT), 12:00pm (EDT), 8:00pm (CEST) Gene therapeutics have great potential to treat many severe diseases in an unprecedented, targeted manner. The biopharmace...
JUN 21, 2022 6:00 AM PDT
JUN 21, 2022 6:00 AM PDT
Date: June 21, 2022 Time: 6:00am (PDT), 9:00am (EDT), 3:00pm (CEST) The global understanding and practice of medicine is currently undergoing a revolutionary change. This shift to precision...
OCT 11, 2022 8:00 AM PDT
C.E. CREDITS
OCT 11, 2022 8:00 AM PDT
Date: October 11, 2022 Time: 8:00am (PDT), 11:00pm (EDT), 5:00pm (CEST) Multiomic profiling of cell populations at single-cell resolution is revolutionizing scientists’ understanding o...
SEP 13, 2022 8:00 AM PDT
SEP 13, 2022 8:00 AM PDT
Date: September 13, 2022 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) Preparedness against pandemic diseases demands rapid-response vaccine technology and ready-to-use analytical methods...
SEP 29, 2022 8:00 AM PDT
SEP 29, 2022 8:00 AM PDT
Date: September 29, 2022 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) Adeno-associated virus (AAV) is one of the most widely used delivery vehicles in gene therapy. To ensure the safety...
MAY 11, 2017 12:00 PM PDT

New multiplexed nCounter® PlexSet™ Reagents- an alternative to qPCR technology

Sponsored by: NanoString Technologies


Show Resources
Loading Comments...
Show Resources
Attendees