MAR 17, 2020 8:00 AM PDT

Multiplexing on Mouse tissue using Cell Signaling Technology antibodies and OPAL IHC method

Speakers

Abstract
DATE:  March 17, 2020
TIME:  8:00am PT, 11:00am PT
 
The determination of target specificity in immunohistochemical analysis requires multiple validation steps. Traditionally, multiplexed immunohistochemistry has been limited to 3 or 4 markers, and cross-reactivity of antibodies can be problematic. Working with murine tissue can present its own set of unique challenges.
 
This webinar will cover the variety of techniques used at Cell Signaling Technology to facilitate antibody validation for both single and multiplex applications, the importance of species-specific validation in immunohistochemistry and the advantages of using rabbit monoclonal antibodies with mouse tissues. We’ll also discuss the advances in multiplex IHC using Opal and rabbit primary antibodies developed by CST to reliably stain for 6 markers in a mouse model of breast cancer and how Akoya Bioscience’s Phenoptics platform can provide quantitative analysis of tumor biology in context.
 
Learning Objectives:
  • Learn to develop Opal IHC Multiplexing strategies on murine tissue regardless of species and without concern for cross-reactivity.
  • Why antibody validation techniques are exceptionally important in multiplex assays and why no single assay is sufficient to verify antibody specificity.
  • The importance of species-specific validation in immunohistochemistry and the advantages of using rabbit monoclonal antibodies with mouse tissues.
  • How Phenoptics with spectral unmixing can remove autofluorescence, produce quantifiable data, and analyze spatial relationships across different species types.
 
 
Webinars will be available for unlimited on-demand viewing after live event.
 
LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.

 


Show Resources
You May Also Like
SEP 14, 2021 7:00 AM PDT
C.E. CREDITS
SEP 14, 2021 7:00 AM PDT
Date: September 14, 2021 Time: 7am PDT, 10am EDT, 4pm CEST A conventional thermal cycler has long been a commodity product in the lab and end-point PCR techniques can be completed almost wit...
MAY 11, 2021 10:00 AM PDT
C.E. CREDITS
MAY 11, 2021 10:00 AM PDT
Date: May 11, 2021 Time: 10:00zm PDT Your samples are some of the most valuable assets in the laboratory. After spending countless hours on extraction and preparation, your conclusions could...
SEP 17, 2021 12:00 PM CST
C.E. CREDITS
SEP 17, 2021 12:00 PM CST
Date: September 16, 2021 Time: 9:00pm (PDT), 12:00am (EDT) 3D cellular models like organoids and spheroids offer an opportunity to better understand complex biology in a physiologically rele...
JUL 15, 2021 8:00 AM PDT
C.E. CREDITS
JUL 15, 2021 8:00 AM PDT
Date: July 15, 2021 Time: 8:00am (PDT), 11:00am (EDT) High dimensional full spectrum flow cytometry grants unprecedented access to previously unattainable parameters in cellular biology. Flu...
OCT 12, 2021 9:00 AM PDT
C.E. CREDITS
OCT 12, 2021 9:00 AM PDT
Date: October 12, 2021 Time: 9:00am (PDT), 12:00pm (EDT) SCIEX’s next-generation Biologics Explorer software is an innovative platform for the comprehensive and deep characterization o...
JUL 15, 2021 9:00 AM PDT
JUL 15, 2021 9:00 AM PDT
Date: July 15, 2021 Time: 9:00am (PDT), 12:00pm (EDT) The Pisces workflow robust, easy-to-use, end-to-end multi-omics solution for highly multiplexed targeted Spatial RNA analysis. VeranomeB...
Loading Comments...
Show Resources