A novel strategy to investigate longitudinal changes within the gut microbiome: Insights from mouse models of sepsis and Alzheimer's disease

C.E. Credits: P.A.C.E. CE Florida CE
Speaker
  • Assistant Professor, Department of Neuroscience and Rockefeller Neuroscience Institute, West Virginia University School of Medicine
    BIOGRAPHY

Abstract

Sepsis is a life-threatening condition that is caused by the immune system’s inability to respond appropriately to an infection. How sepsis can change the gut microbiome in ways that alter the brain and behavior is not well understood. One project in my laboratory is to identify ways to prevent or mitigate the neurological impact of sepsis by understanding how gut microbial communities change in response to sepsis. To investigate this, we compared changes in the gut microbiome between wild type (WT) and Alzheimer’s disease transgenic (CVN-AD) mice who received a sepsis injury. WT and CVN-AD mice were subjected to the cecal ligation and puncture model of sepsis or a sham surgery. Fecal samples were collected from each mouse at baseline (day 0) and 6 days post-sepsis. Each fecal sample was processed in triplicate using Shoreline Biome’s StrainID kit, which features a 16S-23S amplicon product that includes the highly variable internal transcribed spacer (ITS) region. Pooled amplicons (~2500 bp) were sequenced on the PacBio Sequel platform, and reads were demultiplexed using SBAnalyzer software and mapped to the novel Athena database. Dramatic differences in gut microbiome profiles between day 0 and day 6 post-sepsis were observed in both WT and CVN-AD mice. First, a dramatic decrease of the commensal Muribaculum was observed in all septic mice. Second, a unique cluster of septic samples displayed a large increase in Clostridium with a proportional decrease in Muribaculum on day 6. Third, samples with a higher percentage of Lactobacillus at day 0 had a lower decrease in Muribaculum and increase in Clostridium on day 6. WT and CVN-AD mice showed the greatest differences in their abundance of the Lactobacillus strains: L. murinus L. johnsonii, and L. reuteri. These results show that the combination of the StrainID kit, PacBio Sequel, SBAnalyzer software, and Athena database provide an optimal strategy to uncover species- and strain-differences in microbial communities.

Learning Objectives:

1. Explain how sepsis can change the gut microbiome in ways that affect human health and behavior

2. Identify ways that Alzheimer’s disease-like pathology can modify gut microbiome responses during sepsis

3. Describe advantages of 16S-ITS-23S rRNA amplicon sequencing over standard short-read 16S rRNA sequencing


Show Resources
You May Also Like
SEP 14, 2021 7:00 AM PDT
C.E. CREDITS
SEP 14, 2021 7:00 AM PDT
Date: September 14, 2021 Time: 7am PDT, 10am EDT, 4pm CEST A conventional thermal cycler has long been a commodity product in the lab and end-point PCR techniques can be completed almost wit...
JUN 09, 2021 7:00 AM PDT
C.E. CREDITS
JUN 09, 2021 7:00 AM PDT
Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...
NOV 30, 2021 10:00 AM PST
C.E. CREDITS
NOV 30, 2021 10:00 AM PST
Date: November 30, 2021 Time: 10:00am (PDT), 1:00pm (EDT) The prevalence of thyroid disease worldwide has served as a catalyst for healthcare providers to study various tools and methods to...
DEC 09, 2021 11:00 AM PST
C.E. CREDITS
DEC 09, 2021 11:00 AM PST
Date: December 09, 2021 Time: 11:00am (PDT), 2:00pm (EDT) The burden of antimicrobial resistance (AMR) has been acknowledged worldwide by leading health institutes. Besides the need for new...
JUN 22, 2021 10:00 AM PDT
C.E. CREDITS
JUN 22, 2021 10:00 AM PDT
Date: June 22, 2021 Time: 10:00am (PDT), 1:00pm (EDT) Antimicrobial resistance (AMR) has emerged as one of the principal public health problems of the 21st century. It threatens the effectiv...
OCT 20, 2021 10:00 AM PDT
C.E. CREDITS
OCT 20, 2021 10:00 AM PDT
Date: October 20, 2021 Time:10:00am (PDT), 1:00pm (EDT) As the prevalence of Diabetes continues to rise in many areas across the globe, healthcare providers continue to look for methods that...
Loading Comments...
Show Resources