MENU
FEB 03, 2021 12:30 PM EST

Optimizing High-Content Imaging of 3D Models for Drug Discovery

Sponsored by: SBI2, InSphero, Yokogawa
Speakers

Abstract

In vitro spheroid models are fast becoming the de facto standard for drug discovery applications, largely due to their human-like physiological and morphological characteristics, tissue-like cellular complexity, and long culture lifespan, which enables longitudinal studies that better reflect patient treatment plans in the clinic. High content imaging and analysis (HCA) of 3D spheroid models can provide valuable information to help researchers untangle disease pathophysiology and assess novel therapies more effectively. Making the move from simple monolayer 2D cell models to dense 3D spheroids in HCI applications, however, requires 3D-optimized protocols, instrumentation, and resources.

In this webinar, we will discuss considerations for high content imaging and analysis of 3D spheroid disease models for drug discovery, share lessons we learned while in setting up and conducting proof-of-concept studies designed to test the full potential for high resolution image-based analysis of 3D spheroid models, and provide a working checklist for researchers and core services groups planning to exploit these technologies in their work.

Learning Objectives:

  • High Content assat development- considerations when transitioning from 2D cell models to 3D multicellular models including examples of the rich, physiologically-relevant data that can be extracted from multicellular 3D models 
  • 3D image acquisition- high content instrument hardware/software and acquisition settings for efficient acquisition of high quality image stacks
  • 3D image analysis- visualization and image analysis toolsets for 3D imaging, including the application of Deep Learning methods

Show Resources
You May Also Like
SEP 14, 2021 7:00 AM PDT
C.E. CREDITS
SEP 14, 2021 7:00 AM PDT
Date: September 14, 2021 Time: 7am PDT, 10am EDT, 4pm CEST A conventional thermal cycler has long been a commodity product in the lab and end-point PCR techniques can be completed almost wit...
SEP 17, 2021 12:00 PM CST
C.E. CREDITS
SEP 17, 2021 12:00 PM CST
Date: September 16, 2021 Time: 9:00pm (PDT), 12:00am (EDT) 3D cellular models like organoids and spheroids offer an opportunity to better understand complex biology in a physiologically rele...
JUL 15, 2021 9:00 AM PDT
JUL 15, 2021 9:00 AM PDT
Date: July 15, 2021 Time: 9:00am (PDT), 12:00pm (EDT) The Pisces workflow robust, easy-to-use, end-to-end multi-omics solution for highly multiplexed targeted Spatial RNA analysis. VeranomeB...
SEP 29, 2021 11:00 AM PDT
C.E. CREDITS
SEP 29, 2021 11:00 AM PDT
Date: Septembr 29, 2021 Time: 11:00am (PDT), 2:00pm (EDT) 3D cell models are becoming increasingly popular for studying complex biological effects, tissue functionality, and diseases. While...
NOV 09, 2021 11:00 AM PST
C.E. CREDITS
NOV 09, 2021 11:00 AM PST
Date: November 09, 2021 Time: 11:00am (PDT), 02:00pm (EDT) Clinical translation of human pluripotent stem cells (hPSCs) requires advanced strategies that ensure safe and robust long-term gro...
OCT 12, 2021 9:00 AM PDT
C.E. CREDITS
OCT 12, 2021 9:00 AM PDT
Date: October 12, 2021 Time: 9:00am (PDT), 12:00pm (EDT) SCIEX’s next-generation Biologics Explorer software is an innovative platform for the comprehensive and deep characterization o...
Loading Comments...
Show Resources