MENU
MAY 17, 2018 8:00 AM PDT

Optimizing G Protein-Coupled Receptor Expression in Mammalian Cells

Speakers
  • Postdoctoral Researcher, Duke University
    Biography
      Dean Staus received his Bachelor of Science degree in biology from the University of Wisconsin, Whitewater and his PhD in pathology from the University of North Carolina, Chapel Hill. He is currently a postdoctoral researcher in Dr. Robert Lefkowitz's laboratory at Duke University.
    • Postdoctoral Researcher, Duke University
      Biography
        Laura Wingler received her Bachelor of Science degrees in chemistry and biochemistry from North Carolina State University and her PhD in chemistry from Columbia University. She is currently a postdoctoral researcher in Dr. Robert Lefkowitz's laboratory at Duke University.

      Abstract

      G protein-coupled receptors (GPCRs) regulate virtually all known physiological processes and are a premier class of drug targets.  However, a detailed mechanistic understanding of their complex activation mechanisms has been hindered by the difficulty of expressing and purifying these membrane proteins.  We will describe how mammalian expression provides a versatile platform for high-level GPCR expression.  In addition, we will discuss how new expression technologies can address the challenges that have previously limited the scope of mammalian expression systems for biochemical and biophysical studies of GPCRs.


      Show Resources
      You May Also Like
      SEP 10, 2020 9:00 AM PDT
      C.E. CREDITS
      SEP 10, 2020 9:00 AM PDT
      Date: September 10, 2020 Time: 9:00am (PDT), 12:00pm (EDT) Osmolality testing is relevant throughout the entire bioprocessing workflow. As customers look to refine mAb and gene therapy workf...
      APR 07, 2020 8:00 AM PDT
      C.E. CREDITS
      APR 07, 2020 8:00 AM PDT
      DATE: April 7, 2020 TIME: 8:00am PT, 11:00am ET This webinar sets out to establish why quality control is key to robust, reliable, reproducible science. We will look at best practice criteri...
      OCT 08, 2020 7:00 AM PDT
      Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
      C.E. CREDITS
      OCT 08, 2020 7:00 AM PDT
      Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
      DATE: October 8, 2020 TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop thinking about ho...
      MAY 08, 2020 10:00 AM PDT
      C.E. CREDITS
      MAY 08, 2020 10:00 AM PDT
      DATE: May 8, 2020 TIME: 10:00am PT, 11:00am MT, 1:00pm ET The application of next generation sequencing to interrogate immune repertoires and methods in which these highly complex dataset...
      SEP 03, 2020 9:00 AM PDT
      C.E. CREDITS
      SEP 03, 2020 9:00 AM PDT
      DATE: September 3, 2020 TIME: 09:00am PT, 12:00pm ET xxx Learning Objectives: xxx Webinars will be available for unlimited on-demand viewing after live event. LabRoots is approved as a provi...
      JUN 23, 2020 10:00 AM PDT
      C.E. CREDITS
      JUN 23, 2020 10:00 AM PDT
      DATE: June 23, 2020 TIME: 10:00am PT Human mesenchymal stromal or stem cells (MSCs)-based immunomodulation treatment has been proposed as a suitable therapeutic approach for many diseases, s...
      Loading Comments...
      Show Resources
      Attendees
      • See more