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MAY 17, 2018 8:00 AM PDT

Optimizing G Protein-Coupled Receptor Expression in Mammalian Cells

Speakers
  • Postdoctoral Researcher, Duke University
    Biography
      Dean Staus received his Bachelor of Science degree in biology from the University of Wisconsin, Whitewater and his PhD in pathology from the University of North Carolina, Chapel Hill. He is currently a postdoctoral researcher in Dr. Robert Lefkowitz's laboratory at Duke University.
    • Postdoctoral Researcher, Duke University
      Biography
        Laura Wingler received her Bachelor of Science degrees in chemistry and biochemistry from North Carolina State University and her PhD in chemistry from Columbia University. She is currently a postdoctoral researcher in Dr. Robert Lefkowitz's laboratory at Duke University.

      Abstract

      G protein-coupled receptors (GPCRs) regulate virtually all known physiological processes and are a premier class of drug targets.  However, a detailed mechanistic understanding of their complex activation mechanisms has been hindered by the difficulty of expressing and purifying these membrane proteins.  We will describe how mammalian expression provides a versatile platform for high-level GPCR expression.  In addition, we will discuss how new expression technologies can address the challenges that have previously limited the scope of mammalian expression systems for biochemical and biophysical studies of GPCRs.


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