DEC 04, 2018 7:00 AM CET

Pluripotent stem cells: How to raise the quality of your PSC culture

Sponsored by: Eppendorf
C.E. Credits: P.A.C.E. CE Florida CE
Speakers
  • Application Specialist Cell Handling, Eppendorf AG
    Biography
      Dr. Jessica Wagener is Application Specialist focusing on Cell Handling at Eppendorf Headquarter in Hamburg, Germany. Entering a cell culture laboratory as a graduate student for the first time in 2006, she came to Eppendorf with 5+ years of experience in cell culture. After studying Biology at the Universities of Marburg and Düsseldorf, she investigated apoptosis mechanisms in different cell lines for her diploma thesis. Following one year as Research Associate working with primary cells she started to work towards her PhD with a focus on reproductive biology at the Medical School Essen. She joined Eppendorf in April 2012 shortly after completing her PhD in Biology.
    • Application Specialist Manual Liquid Handling, Eppendorf AG
      Biography
        Dr. Nadine Mellies is Application Specialist focusing on Manual Liquid Handling at Eppendorf Headquarter in Hamburg, Germany. She studied Biology at the University of Oldenburg, Germany, investigating visual signal processing in the carp retina in her master thesis. Nadine Mellies holds a PhD in Biology with a focus on male reproductive biology working with mouse and human stem cells at the Institute of Human Genetics Goettingen, Germany. After completing her PhD thesis, she joined Eppendorf in June 2016 with 4+ years of experience in cell culture applications.

      Abstract
      DATE:    December 4, 2018
      TIME:    4:00pm CET
       
      PSCs represent an important tool in a wide range of applications, including basic research, disease modeling, drug discovery, and regenerative medicine. As stem cell cultures are very sensitive and respond to the smallest influence, stable culture conditions are crucial to maintain reliability and reproducibility of experimental data. In this educational webinar we will look at the different cultivation parameters, which can affect the viability and proliferation of PSCs during expansion. Join us to learn more about how to assess the quality of your stem cells and to preserve their pluripotency.
       
      Key learning objectives:
      • Important parameters for PSC culture: growth surface, medium, and culture environment
      • Assessing stem cell quality: PSC characteristics and genetic stability
      • Improving reproducibility: Detecting cryptic contamination (e.g. Mycoplasma, cross-contamination)

      Show Resources
      You May Also Like
      OCT 08, 2020 7:00 AM PDT
      C.E. CREDITS
      OCT 08, 2020 7:00 AM PDT
      DATE: October 8, 2020 TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop thinking about ho...
      DEC 02, 2020 8:00 AM PST
      C.E. CREDITS
      DEC 02, 2020 8:00 AM PST
      DATE: December 2nd, 2020 TIME: 08:00am PDT, 11:00pm EDT Bioreactors and shakers are used to cultivate microorganisms, plant, insect, and mammalian cells in different volumes. Upscaling of pr...
      SEP 10, 2020 9:00 AM PDT
      C.E. CREDITS
      SEP 10, 2020 9:00 AM PDT
      Date: September 10, 2020 Time: 9:00am (PDT), 12:00pm (EDT) Osmolality testing is relevant throughout the entire bioprocessing workflow. As customers look to refine mAb and gene therapy workf...
      AUG 25, 2020 8:00 AM PDT
      C.E. CREDITS
      AUG 25, 2020 8:00 AM PDT
      DATE: August 25, 2020 TIME: 8:00am PDT, 10:00am CDT, 11:00am EDT Recombinant lentivirus (LV) and adeno-associated virus (AAV) are critical components of cell and gene therapies, which show g...
      NOV 16, 2020 8:00 AM PST
      C.E. CREDITS
      NOV 16, 2020 8:00 AM PST
      Date: November 16, 2020 Time: 8:00am (PST), 11:00am (EST) CRISPR screening has become the prime discovery tool in modern biomedical research and drug discovery. At the same time, most screen...
      OCT 29, 2020 6:00 AM PDT
      C.E. CREDITS
      OCT 29, 2020 6:00 AM PDT
      Date: October 29, 2020 Time: 6:00am (PDT), 9:00am (EDT), Chronic inflammation can occur as a result of a combination of genetic predispositions and environmental factors. Epigenetic modifica...
      Loading Comments...
      Show Resources
      Attendees
      • See more