OCT 30, 2018 9:00 AM PDT

Practical Considerations in the Design of Bottom-Up Mass Spectrometry Based Proteomics Methods

Speaker

Abstract

DATE: October 30, 2018
TIME:  9:00AM PST

Mass spectrometry-based proteomics is a rapidly growing area of research that provides useful information for many fields including basic molecular biology, clinical diagnostics, and pharmaceutical drug development. In all of these fields, accurate quantification is pivotal to ensuring high data quality. This presentation will focus on using mass spectrometry to develop of quantitative assays for both targeted and untargeted bottom-up proteomics experiments. We will discuss experimental design for proteomics experiments and how to select your data acquisition mode. Methods for data collection will be presented with a focus on ways to ensure accurately quantitative values and common misconceptions and pitfalls of the data will be addressed. Techniques for system suitability and quality control will be presented and the use of these methods for quality assurance and standardization will be discussed.

Learning Objectives:

  • Key characteristics of quantitative mass spectrometry proteomic assays
  • Selection of peptides that are stable, precise, and provide linear response in the sample matrix
  • Use of system suitability and quality control guidelines for quantitative mass spectrometry methods

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OCT 30, 2018 9:00 AM PDT

Practical Considerations in the Design of Bottom-Up Mass Spectrometry Based Proteomics Methods



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