OCT 30, 2018 9:00 AM PDT

Practical Considerations in the Design of Bottom-Up Mass Spectrometry Based Proteomics Methods

Speaker
  • Proteomics Platform Manager, BC Cancer Research Centre, Michael Smith Genome Sciences Centre
    Biography
      Dr. Spencer graduated cum laude with BS degrees in chemistry and biochemistry from the University of Texas at Arlington in 2010. During this time she did research with Dr. Kevin Schug investigating the toxic components secreted by the fish-killing algae P. parvum. She continued on to do her graduate work in aerosol chemistry and mass spectrometry instrumentation under the supervision of Dr. Gary Glish at the University of North Carolina at Chapel Hill, writing her thesis on real-time analysis of aerosol particle composition using novel ambient ionization and gas phase separation techniques. Sandi did her postdoctoral research in the MacCoss laboratory at the University of Washington. Here she primarily worked on method development for targeted proteomics and improvement of nanoLC robustness by implementing robotic solutions. She is currently the Proteomics Platform Manager in the Morin lab at the BC Cancer Research Centre.

    Abstract

    DATE: October 30, 2018
    TIME:  9:00AM PST

    Mass spectrometry-based proteomics is a rapidly growing area of research that provides useful information for many fields including basic molecular biology, clinical diagnostics, and pharmaceutical drug development. In all of these fields, accurate quantification is pivotal to ensuring high data quality. This presentation will focus on using mass spectrometry to develop of quantitative assays for both targeted and untargeted bottom-up proteomics experiments. We will discuss experimental design for proteomics experiments and how to select your data acquisition mode. Methods for data collection will be presented with a focus on ways to ensure accurately quantitative values and common misconceptions and pitfalls of the data will be addressed. Techniques for system suitability and quality control will be presented and the use of these methods for quality assurance and standardization will be discussed.

    Learning Objectives:

    • Key characteristics of quantitative mass spectrometry proteomic assays
    • Selection of peptides that are stable, precise, and provide linear response in the sample matrix
    • Use of system suitability and quality control guidelines for quantitative mass spectrometry methods

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